Postharvest losses are a significant concern for tomato breeding associated with their short fruit shelf life. Sletr1-2 is a new ethylene receptor mutant that has a prominent character in a prolonged fruit shelf life. This research aimed to estimate the combining ability of Sletr1-2 mutant and determine the selection method for future breeding associated with the fruit shelf-life and yield. Four lines of tropical tomato, i.e., 'Intan,' 'Mirah,' 'Ratna' and 'Mutiara,' were crossed with the wild type Micro-Tom (WT-MT) and Sletr1-2 mutant tomato using a line x tester mating design. A randomized complete block design with four replications was used to evaluate twelve F1 and their parents. The study revealed significant differences in the GCA of the line and tester but not for SCA. The lines and testers contributed more to total variance than their interaction. 'Intan' and Sletr1-2 mutants had the greatest fruit shelf-life combiners, with additive gene action being the most prevalent. Simple phenotypic selection or pure line selection from selected crosses in advanced generations would be preferable. 'Mutiara' was the best combiner for yield and plant growth, with non-additive gene action was the most common. The breeding strategy that considered dominance, overdominance, and epistasis was preferred.
Pemilihan desa terbaik setiap tahun dilakukan oleh pemerintah Kecamatan, lomba ini dapat memotivasi warga dan perangkat desa untuk membuat desanya menjadi lebih baik. Kendala yang dialami selama ini adalah apabila terjadi hasil penilaian sama persis maka akan sulit menentukan desa mana yang mendapat predikat sebagai desa terbaik, sehingga menyulitkan tim penilai untuk menentukan juaranya apabila desa yang memiliki nilai sama adalah desa yang paling tinggi nilainya. Tujuan dari penelitian ini adalah untuk membuat sistem pendukung keputusan pemilihan desa terbaik dengan metode ARAS. bahasa pemrograman yang digunakan adalah PHP dan MYSQL sebagai databasenya.
Abstract. Prayoga MK, Syahrian H, Rahadi VP, Atmaja MIP, Maulana H, Anas. 2022. Quality diversity of 35 tea clones (Camellia sinensis var. sinensis) processed for green tea. Biodiversitas 23: 810-816. The development of sinensis clones in Indonesia is still focused on increasing productivity and until now there has been no study on the level of genetic similarity and diversity based on quality characters. Therefore, the purpose of this study was to examine the level of kinship and genetic diversity of 35 sinensis clones from the collection of the Indonesia Research Institute for Tea and Cinchona. The study consisted of two stages, testing the content of chemical compounds (polyphenols and caffeine) and organoleptic testing. The material used in both tests was fresh shoots consisting of peko + 3 young leaves (P+3). In organoleptic testing, shoots of 35 sinensis variety clones were first processed into green tea. Furthermore, the processed green tea was tested organoleptically by three credible panelists. The data from the test results of chemical and organoleptic compounds were then tested statistically to get the coefficient of variation (CV), which indicates data diversity. The level of genetic closeness between clones based on quality characters was analyzed using the JMP 16.0 program (trial version), then arranged into clusters. To see the contribution of each parameter to the quality diversity of 35 sinensis clones, a principal component analysis (PCA) was carried out. The results of this study indicate that there are four main clusters grouping the 35 sinensis clones tested. The first cluster excels in the character of taste and aroma, the second cluster excels in appearance, seductive colour, and infusion, the third cluster excels in the character of polyphenols, while the fourth cluster excels in the character of caffeine. The characters that contributed to the genetic diversity of the 35 clones tested were caffeine, appearance, and aroma.
Karakter daya hasil tinggi adalah salah satu target utama dalam pemuliaan padi. Pengujian daya hasil merupakan salah satu tahapan uji dalam proses seleksi varietas baru. Seleksi pada karakter daya hasil merupakan proses yang kompleks karena berkaitan dengan karakter kuantitaif. Pemilihan metode yang tepat akan meningkatkan tingkat keberhasilan proses seleksi, sehingga tujuan penelitian ini adalah untuk menyeleksi genotip padi yang memiliki potensi hasil tertinggi tercapai. Percobaan dilaksanakan pada bulan Juli 2018 sampai Maret 2019 di Kebun Percobaan Ciparanje Fakultas Pertanian, Universitas Padjadjaran, Jatinangor, Sumedang Jawa Barat. Material genetik yang digunakan adalah lima famili padi generasi F4, lima famili generasi F6, dan empat varietas padi sebagai cek. Keseluruhan karakter agronomis dan komponen hasil dievaluasi menggunakan uji Least Significant Increase (LSI), dan indeks seleksi (indeks Mulamba dan Mock, dan indeks jarak genotipe-ideotipe). Berdasarkan hasil uji LSI, famili PP48-5 dan famili SP101-3-1-5 menunjukkan penampilan terbaik pada hampir keseluruhan karakter dibandingkan dengan rata-rata terendah varietas pembandingnya. Berdasarkan nilai indeks seleksi hanya famili SP101-3-1-5 yang memiliki nilai indeks seleksi daya hasil tertinggi. SP101-3-1-5 terpilih sebagai famili yang berpotensi sebagai padi berdaya hasil tinggi.
Genetic diversity measurement of Paraserianthes falcataria half-sib progeny is required to study the genetic quality to support tree improvement programme, plantation and seed source development in tree breeding. To investigate the genetic diversity of half-sib progeny, Random Amplified Polymorphic DNA (RAPD) molecular marker can be utilized. This study aimed to estimate the genetic diversity of P. falcataria half-sib progeny population from four different provenances, i.e., Banjarnegara provenance, Subang provenance, Kediri provenance, and Kuningan provenance, following selection to stem borer and growth increment. Leaf samples of 160 seedlings were collected from 16 parents, four parents from each origin. Genetic diversity was analyzed using four RAPD primers with 30 polymorphic loci. The result showed that the genetic diversity of the half-sib progeny population for each parameter was PP/L=1.82, PPL=77.50%, and He=0.240. Genetic distance among the population was ranged between 0.025 and 0.140 by Gregorius (1974), on the other hand, the genetic distance among the population was varied between 0.094 and 0.266 by Nei (1972). Some half-sib progeny from the same parents were grouped in the same cluster. Therefore, the clustering of the provenance had no relationship with the source of geographic distribution. Genetic relationship among half-sib progeny population was divided into three groups by Nei (1972). The first group consisted of Banjarnegara and Subang half-sib progeny, the second group was Kediri half-sib progeny, and the third group was Kuningan half-sib progeny.
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