Pemanfaatan Marka Rapd Untuk Mengestimasi Diversitas Genetik Progeni Half-Sib Paraserianthes falcataria (L.) Nielsen Hasil Seleksi Ketahanan Boktor Dan Riap Pertumbuhan

Abstract

Genetic diversity measurement of Paraserianthes falcataria half-sib progeny is required to study the genetic quality to support tree improvement programme, plantation and seed source development in tree breeding. To investigate the genetic diversity of half-sib progeny, Random Amplified Polymorphic DNA (RAPD) molecular marker can be utilized. This study aimed to estimate the genetic diversity of P. falcataria half-sib progeny population from four different provenances, i.e., Banjarnegara provenance, Subang provenance, Kediri provenance, and Kuningan provenance, following selection to stem borer and growth increment. Leaf samples of 160 seedlings were collected from 16 parents, four parents from each origin. Genetic diversity was analyzed using four RAPD primers with 30 polymorphic loci. The result showed that the genetic diversity of the half-sib progeny population for each parameter was PP/L=1.82, PPL=77.50%, and He=0.240. Genetic distance among the population was ranged between 0.025 and 0.140 by Gregorius (1974), on the other hand, the genetic distance among the population was varied between 0.094 and 0.266 by Nei (1972). Some half-sib progeny from the same parents were grouped in the same cluster. Therefore, the clustering of the provenance had no relationship with the source of geographic distribution. Genetic relationship among half-sib progeny population was divided into three groups by Nei (1972). The first group consisted of Banjarnegara and Subang half-sib progeny, the second group was Kediri half-sib progeny, and the third group was Kuningan half-sib progeny.

Phenotypic and Genetic Diversity Evaluation of Sengon (Falcataria moluccana (Miq.) Barneby & JW Grimes) from Solomon Provenance on Progeny Trial in Cirangsad Experimental Forest, West Java

“…(a) The amplified DNA of sengon using primer OPA-05, (b) The amplified DNA of sengon using primer OPA-14, and (c) The amplified DNA of sengon using primer OPY-16. higher mean of genetic diversity than the sengon half-sib progeny population (He = 0.244) analyzed with the RAPD marker by Rostini et al (2018) and genetic diversity of healthy-growing sengon populations (He = 0.262) in various locations on the Java Island which was analyzed by Lelana et al (2018) using the RAPD marker. This pattern indicated that sufficient of the diversity source in sengon from Solomon provenance that grows in Pompok Landak Permanent Nursery-Haurwangi Village at this time.…”