Background: The uterus is a major female hormone-responsive reproductive pear-shaped organ located within the pelvic region. The gross morphology of uterus quite dramatically changed throughout life by changing levels of ovarian hormones. A hysterectomy is done for many reasons including the ridding of tumours both benign and malignant. It is the most commonly performed gynecological surgical procedure throughout the world. Materials and Methods: This study was undertaken to identify the different types of pathologies in hysterectomy specimens and to correlate the findings with the different age group and clinical indications. In the present study, 303 cases were studied over a period of two years from January 2016 to December 2017. Surgical specimens were formalin fixed and the tissue was adequately processed for histopathological examination. The sections were stained routinely with hematoxylin and eosin stain. Results: Of the 303 cases, half of the cases (50.8%) were encountered in the age group of 40-49 years which was the most common age group. Fibroid uterus (38.6%) and uterine prolapse (32.6%) were the most common clinical indications for hysterectomy. The most common pathology identified was leiomyoma (42.2%) in myometrium. In cervix most common finding was chronic cervicitis (28.1%). Histopathological examination confirmed the clinical and gross diagnosis in majority of the cases. Conclusions: The present study provides a fair insight into the histological patterns of lesions in hysterectomy specimens in our institution. A wide range of lesions are encountered when hysterectomy specimens are subjected to histopathological examination. Few lesions were encountered as incidental findings. Hence, it is important that every hysterectomy specimen should be subjected to detailed gross and histopathological examination for better postoperative management.

Introduction: Lymph node enlargement is frequent presentation in all age groups with a wide spectrum of diseases, ranging from infections to malignancy. Therefore, management of cases depends on lymph node pathology, which can be studied by collecting material through fine needle aspiration method that can be used as an outpatient procedure. Objectives: The study was undertaken to assess the cytomorphological features and incidence of various lymph node diseases on fine-needle aspiration cytology (FNAC) and to analyze the utility and diagnostic importance of FNAC in lymph node diseases. Materials and Methods: In the study, total of 250 patients were selected who had presented with lymph node enlargement at Department of Pathology in our Tertiary Care Centre. Results: In this series of FNAC, cervical lymph nodes were involved in majority of 171 (68.4%) cases. The age of patients ranged from 6 months to 92 years with slight male preponderance. Tubercular lymphadenitis comprised the majority (50.8%) followed by reactive lymphoid lesions (20.8%), metastatic malignancies and acute suppurative lymphadenitis (8.8%) each, granulomatous lymphadenitis (8.4%), chronic nonspecific lymphadenitis (1.2%), necrotizing lymphadenitis (0.8%) and lymphoma (0.4%). Conclusion: FNAC is a simple, safe, reliable, inexpensive and the most diagnostic tool in early detection of lymph node lesions.

#1008 Background: Touch imprint cytology (TIC) as a tool for intra-operative assessment of sentinel lymph nodes (SLN) in breast cancer has been shown to be rapid, reliable and cost-effective. Its use in lobular cancer is however more challenging due to the pattern of lymph node infiltration, smaller cells, similarity to lymphocytes and low grade cytomorphology. The aim of this study was to evaluate the sensitivity and specificity of intra-operative TIC in assessing SLN metastases in patients with lobular breast cancer.
 Material and Methods: All patients with lobular carcinoma who underwent intra-operative TIC assessment of the SLN were identified within our prospectively collected database. The SLN was bi-valved along its longitudinal axis and imprinted onto a slide at least six times before air-drying. The slide was treated with the rapid Giemsa stain before examination by one of three cytopathologists. Where TIC revealed SLN metastases, the patient underwent immediate axillary clearance. The SLN were then processed for histopathology by sectioning at three levels before haematoxylin and eosin staining. Those lymph nodes found to be negative then underwent immunocytohistochemistry staining before being declared truly negative. All patients with SLN metastases not identified during surgery received a delayed axillary clearance. The TIC results were compared to the final histopathology result of the SLN and the sensitivity and specificity calculated.
 Results: 51 patients with lobular cancer who underwent TIC were identified. The table below summarises the results:
 
 The sensitivity thus calculated was 76.0%; the specificity 100.0% and the negative predictive value 81.25%. Of the 6 false negative cases, 4 were grade 1 and 2 were grade 2. The mean primary tumour size in this group was 17mm (range 11 to 27mm). In all 6 cases, axillary metastases was found in 3 or fewer lymph nodes post axillary clearance.
 Discussion: This study demonstrates the reliability of using intra-operative TIC to evaluate SLN during surgery for lobular cancer. It compares very favourably to data published in the literature from other institutions that have examined the use of TIC in lobular carcinoma and may relate to the presence of experienced, highly motivated cytopathologists at our unit. In addition, it also compares well with other studies describing the use of TIC in ductal carcinoma, including our own unit's previously published data. Thus, in experienced hands, TIC is feasible with an acceptable accuracy allowing intra-operative SLN assessment in patients with lobular breast cancer. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 1008.

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