Vertical and seasonal distributions of flying beetles were investigated in a suburban temperate deciduous forest in Kanazawa, Japan using water pan traps to determine the abundance and composition among vertical strata, change in the abundance and composition through seasons and determinant factors in generating the distributions. Traps were placed at three levels (0.5 m, 10 m, and 20 m above ground) on a tower. Samplings were carried out seasonally from May to November in 1999 and 2000. Variations in the abundance of flying beetles were observed from different layers. The results showed that the abundance and composition of flying beetles varied among strata and seasons. In both 1999 and 2000, Elateridae was consistently most abundant in the bottom layer, while Attelabidae and Cantharidae were most abundant in the upper layer. In 1999, Eucnemidae and overall scavengers were most abundance in the bottom layer, but results were not consistent with those in 2000. In general, the abundance of herbivores reaches a peak in the early season (May/June) and decreases in the following months. Peaks of abundance in predators vary vertically. In the bottom layer a peak was observed in the early season (May/June), while in the upper layer this was observed in July. Scavengers had two peaks, in May/June and September. These patterns indicated that vertical distributions in the abundance of different feeding guilds varied through seasons.

Meru Betiri National Park (MBNP) is one of the nature conservation area that has the potential of flora, fauna, and ecosystems that could develop as a nature-based tourism attraction. The existence of certain indicator species was related to estimation of stress level and disturbance on ecosystem stability for making strategic decisions about the restoration in this area. One of the important indicator species at forest ecosystem were soil arthropods. Aim this research were analyzed composition and diversity of soil arthropods at Rajegwesi, MBNP areas. The methods in this research used pitfall trap, measurement of distribution structure and soil arthropods composition based on the Shannon -Wiener index, Morisita similarity index and Importance Value Index (IVI). The number of families and individuals of soil arthropods found in the coastal area of Rajegwesi consists of 10 order with 21 families (702 individual). The number of individuals of the order Hymenoptera, Coleoptera, Collembola and Araneida was more widely found. Soil arthropods diversity index on each land use indicated that soil arthropod diversity in these areas were moderate. Soil arthropod community of orchards and forest had a similarity of species composition, whereas soil arthropod community of savanna had a similarity of species composition with paddy fields.

 Abstract-Cadmium (Cd) is a heavy metal that be a source of concern for industrial workers and it was proposed in the formation of advance glycation end products (AGEs) such as methylglyoxal (MG). MG have recently attracted much attention because of their possible clinical significance in chronic and agerelated diseases. Based on previous research, methylglyoxal formation can be accelerated by metals in vitro. The role of Cd in the formation of MG and hydrogen peroxide has not been much studied. Thus, our study aims to measure the formation rate of MG and hydrogen peroxide in the presence of Cd in vitro. This research was divided into 4 groups (1 control group and 3 treatment groups), than we set carbonyl compound assay, methylglyoxals assay, and hydrogen peroxide assay. For analyzing of the data, SPSS software version 17 was used and was examined by ANOVA and regression correlation test. For all outcomes, a nominal p-value of p < 0,05 was considered significant. We found that there are significant correlation between Cd exposure on the formation of hydrogen peroxide and methylglyoxal (p < 0,05) in nonenzymatic glycation of proteins by glucose. The increased Cd level accelerate the formation of methylglyoxal and hydrogen peroxide.

Abstract. Leksono AS, Yanuwiadi B, Afandhi A, Farhan M, Zairina A. 2020. The abundance and diversity of grasshopper communities in relation to elevation and land use in Malang, Indonesia. Biodiversitas 21: 5614-5620. Ecological factors include interactions of the community members with numerous biotic and abiotic factors such as temperature, humidity, precipitation, light intensity, and seasonality show an altitudinal gradient. Most grasshopper species play a role as herbivores and are a good source of protein for other animals such as amphibians, small reptiles, birds, and small mammals. This study aimed to analyze variations in the abundance, richness, and diversity of grasshopper species along an altitudinal gradient. This research was conducted in five locations in Malang District, East Java, Indonesia, namely Bantur, Sumber Pujung Lawang, Pujon, and Poncokusumo. Grasshopper sampling was carried out by the sweeping method using an insect net. Sweepings were carried out on four plots with each plot size of 2 x 10 m2. Sampling was conducted four times from June to August 2020. The data were analyzed using the Shannon Wiener index (diversity analysis) and the Bray-Curtis index. The differences between locations were tested by one-way analysis of variance. Land use was analyzed by ArcGIS, using Landsat imagery 8. The abundance of grasshoppers had a significant negative correlation with elevation. That correlation was positive to species richness and diversity of grasshoppers. That with species diversity was significant, while that with species richness was not significant. The greatest abundance of the grasshopper was found in the middle elevation in Lawang (19.39 ± 2.12). In contrast, the highest species richness and diversity were found in the highest elevation in Poncokusumo (richness = 15.75 ± 1.60 and H '= 2.58 ± 0.11). Land use variation was not significant on abundance, species, richness, and diversity of grasshoppers. Interestingly, the high similarity of the grasshopper compositions in low elevation habitats was detected, indicating that land use in the low land area was remarkable. The abundance of grasshopper had a significant positive correlation with temperature but negatively correlated with humidity. There was no significant correlation among species richness, species diversity with temperature and humidity.

Asteraceae family has various benefit as herbal medicine and phytochemical affect (biopesticides). It can grow in different habitats but the morphological and physiological characters of Asteraceae depend on the environmental factor. The aims of the study is to describe the variation of height of stems and width of leaves from three species of Asteraceae family (Pluchea indica, Ageratum conyzoides and Elephantopus scaber), on three types of habitat which differed by its altitude and learn the correlation between altitude and morphological characteristics of Asteraceae. Samples of Asteraceae were obtained from Bangkalan-Madura The habitat altitude and the environmental factors were determined by measuring altitude, light intensity, oxygen levels (climate), organic compound, C level, N level, pH (soil chemical character), soil temperature, water levels, porosity, dust, sand and soil humidity (soil physical character). Asteraceae which grows at intermediate-altitude has the highest stems. While, Asteraceae at low-altitude has the widest leaf.

Watulimo District, Trenggalek Regency is one of the area in East Java Province that has an abundant marine potential. However, this huge potential has not been optimally developed in a form of marine tourism. This study aims to determine internal and external factors that influence the development of marine tourism in Watulimo Subdistrict, Trenggalek, and develop a strategy of marine tourism development in a sustainable manner. This study was conducted on 4 beaches in Watulimo Subdistrict, Trenggalek Regency: Prigi, Karanggongso, Cengkrong, and Damas. Interviews were conducted by using semi-structured technique. Secondary data is obtained from government policy documents relating to tourism; tourism infrastructure facilities in the research location; data of tourist visits; geography and demographics; socio-cultural and economic data. Most respondents believe that promotion (0.083) and tourist information center (0.075) indicator are considered very important. Advances in information technology have the highest weight (0.097), where respondents can know about the state of the marine tourism area based on information and reviews. The results show the number of scores obtained from the weighting and external factor rating of 2.656 (good value range), which illustrates that the marine tourism area in Watulimo has an opportunity in its development. Cooperation with travel agents, hotels, restaurants, and tourist information centers for Trenggalek District can be incorporated into their brochures to increase traffic.

Abstract. Leksono AS, Yanuwiadi B, Khotimah A, Zairina A. 2021. Grasshopper diversity in several agricultural areas and savannas in Dompu, Sumbawa Island, Indonesia. Biodiversitas 23: 75-80. In Sumbawa Island, the conversion of forests and savannahs into agricultural land has increased rapidly since 2010. This research aims to compare grasshopper species' abundance, richness, and diversity between several farmland and savannas in Dompu, Sumbawa Island. It was conducted at ten locations in Dompu, Sumbawa Island, and included an ecotone, two post-harvested rice farms, two post-harvested corn farms, a mixed farm, a vegetable farm, and three savannas. Furthermore, samples were taken four times from four plots at each location in the post-harvest period from August to September 2021. Grasshopper sampling was carried out using the sweeping method with an insect net with each plot size of 2 x 10 m2. A total of 2264 individual grasshoppers belonging to 30 species and four families were collected from all research sites. The dominant species were Alloteratura sp., Trilophidia annulata, Atractomorpha crenulata, Phlaeoba fumosa, Oxya japonica and, Phlaeoba infumata. The greatest grasshopper species richness and diversity were found in post-harvest rice farms, while the lowest was in the vegetable farm, and most of these species are considered pests. This research shows that the composition of grasshoppers on agricultural land is very similar to that of the adjacent savannah. Hence, monitoring and controlling their presence is necessary by paying attention to savannas as refuge land.

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