Tuberculosis is a chronic infectious disease caused by the Mycobacterium tuberculosis complex (CMT), members including M. bovis. The sanitary inspection in slaughterhouses has a great importance on public health for the removal of contaminated meat with pathological lesions. Cattle slaughtered in the abattoir of Garanhuns were subjected to macroscopic, histological, bacteriological, and molecular analyses. Lung inspection revealed gross lesions suggestive of tuberculosis. The characterization of tuberculous granulomas was performed by histopathology and hematoxylin and eosin (HE) staining. The identification of acid-fast bacilli (AFB) was performed on smears with fresh material and histological staining using Ziehl-Neelsen stain. Bacteriological diagnosis was carried out using Stonebrink and Lowenstein-Jensen media, and polymerase chain reaction (PCR) was performed from the DNA extracted from colonies for the identification of Mycobacterium spp. We found that 1% (32/3,.180) of the collected lungs showed lesions suggestive of tuberculosis. Histopathological analysis revealed that 65.62% (21/32) samples had granulomatous pneumonia. AFB was detected in 46.88% (15/32) smears and 4.16% (1/24) histological sections, as analyzed by Ziehl-Neelsen staining. Moreover, 68.75% (22/32) samples showed bacterial growth and molecular identification 90% (18/20) of the samples were positive for M. bovis and 10% (2/20) for Mycobacterium sp. Thus, microbiological culture and PCR analyses revealed the high rate of bovine tuberculosis in this region, indicative of the need to disclose these results in the interest of public health. Consequently, we emphasize the need for an accurate macroscopic evaluation of lesions suggestive of bovine tuberculosis. Key words: Granuloma. Pneumonia. Public health. ResumoA tuberculose é uma doença infecciosa crônica causada por membros do complexo Mycobacterium tuberculosis (CMT), incluindo M. bovis. A inspeção sanitária em matadouros frigoríficos possui grande importância para a saúde pública pela remoção de carne contaminada com lesões patológicas. Os bovinos abatidos no matadouro de Garanhuns-PE foram submetidos a análises macroscópicas, histológicas, bacteriológicas e moleculares. A inspeção pulmonar revelou lesões sugestivas de tuberculose.A caracterização de granulomas tuberculoides foi realizada através da histopatologia e coloração de hematoxilina e eosina (HE). A identificação de bacilo álcool -ácido resistente (BAAR) foi realizado em esfregaços com material fresco e em cortes histológicos sob a coloração de Zihel-Neelsen. O diagnóstico bacteriológico foi realizado em meio de cultivo Stonebrink e Lowenstein-Jensen e a reação em cadeia da polimerase (PCR) sucedeu a partir de DNA extraído de colônias para identificação do Mycobacterium sp... Verificou-se que1% (32/3.180) dos pulmões coletados apresentaram lesões sugestivas de tuberculose. Na análise histopatológica 65.62% (21/32) amostras tinham pneumonia granulomatosa. BAAR foram detectados em 46,88% (15/32) esfregaços e 4,16% (1/24) ...

RESUMO: O Carcinoma de Células Escamosas é um tumor maligno dos queratinócitos.No equino representa de 6-10% de todas as neoplasias que afetam a genitália externa. Os sinais geralmente incluem placas despigmentadas, irregularidades da superfície do pênis ou prepúcio, erosões incuráveis com ou sem tecido de granulação acompanhado e casos de metástase são observados em aproximadamente 18% das ocorrências. Microscopicamente observa-se formação de pérolas de queratina, mitoses e atipia celular. O diagnóstico é feito a partir dos sinais clínicos, achados histopatológicos, ultrassonografia e punção aspirativa. Relata-se um caso de um equino, macho, castrado, 13 anos, SRD, pelagem alazão, que foi atendido no Hospital Veterinário do Campus Palotina da Universidade Federal do Paraná. Devido ao mau estado corporal o animal foi submetido à eutanásia, sendo realizada a necropsia e exame histopatológico dos órgãos que foram corados pela coloração de Hematoxilina e Eosina. Na necropsia foi observada uma massa irregular ulcerada, firme, branco-amarelada e com superfície de aspecto granular no pênis e prepúcio. Os pulmões indicaram presença de metástase. No histopatológico confirmou-se que as lesões eram metástases do carcinoma de células escamosas. O objetivo deste trabalho é relatar um caso de carcinoma de células escamosas em pênis e prepúcio de equino com metástase pulmonar. Palavras-chave: histopatológico, neoplasia, pênisABSTRACT: Squamous Cell Carcinoma is a malignant tumor of keratinocytes. In the horse represents 6-10% of all cancers affecting the external genitalia. The signals typically include unpigmented plaques, surface irregularities of the penis or the prepuce, incurable with or without erosions accompanied by granulation tissue and cases of metastasis are observed in approximately 18% of cases. Microscopically observed formation of keratin, mitoses and cellular atypia. Diagnosis is made from clinical signs, histopathological findings, ultrasound and needle aspiration. We report a case of a horse, male, neutered, 13 years, SRD, sorrel coat, which was the Veterinary Hospital of Palotina Campus of Federal University of Paraná. Due to poor body condition the animal was euthanized, necropsy and histopathological examination of organs which were stained with hematoxylin and eosin staining being held. At necropsy an ulcerated, firm, white-yellowish and granular appearance on the surface of the penis and foreskin irregular mass was observed. The lungs showed metastasis. In the histopathological it was confirmed that the lesions were metastases, squamous cell carcinoma. The objective of this study is to report a case of squamous cell carcinoma of the penis and foreskin in equine pulmonary metastasis.

The aim of this study was to evaluate the main nonconformities that result in cytopathological samples inappropriate for diagnosis in veterinary medicine. All cytopathological samples, obtained from different canine and feline tissues/lesions, included in the study were received and classified as inconclusive by a single public laboratory of veterinary pathology, located in Pernambuco State, Brazil, between 2012 and 2016. Nonconformities regarding the smear quality, cellularity, presence or absence of hemorrhage, cellular overlapping, desiccation, and presence or absence of necrotic debris and/or artifacts were evaluated. Data were tabulated using Microsoft Excel 2007; absolute and relative frequencies were calculated using EPIINFO 3.5.2. From the 3268 cases received between 2012 and 2016, 190 cases were selected and comprised 514 inconclusive slides. The most frequent nonconformities detected were insufficient/absence of cellularity in 100% (514/514), inadequate submacroscopic presentation in 87% (446/514), and hemorrhage in 69% (356/514) of samples. Other features identified were cellular overlapping in 34% (175/514), inadequate staining in 31% (175/514), artifacts in 30% (154/514), desiccation in 28% (145/514), and necrotic debris in 26% (133/514) of samples. The implementation of laboratory standard operational procedures aimed at maintaining quality is essential. It is necessary to initially identify the main errors occurring in the processing stages as a way to guide and design strategies to avoid them.

Os ependimomas são neoplasias benignas ou malignas de células ependimárias que revestem o plexo coroide, o assoalho dos ventrículos cerebrais e o canal medular. São raros em animais domésticos, mas há relatos em cães e gatos. Neste relato descreve-se um carcinoma metastático de células ependimárias diagnosticado em equino Mangalarga Marchador, com morte decorrente de choque circulatório. Nódulos de superfície levemente rugosa, firme e coloração parda presentes no plexo coroide do IV ventrículo, rim e pulmão foram coletados, fixados em solução de formalina tamponada a 10%. Os fragmentos foram processados com inclusão em parafina e coloração em Hematoxilina/Eosina (HE) e imunomarcados para detecção de GFAP, vimentina e AE1/AE3. Os nódulos do IV ventrículo continham células ependimárias com anisocitose, anisocariose e figuras de mitose. Este padrão celular foi observado no rim e pulmão. Na imunohistoquímica, o nódulo do IV ventrículo foi positivo para GFAP, negativo para vimentina e fortemente positivo para AE1/AE3. No rim e pulmão os fragmentos foram positivos apenas para AE1/AE3. A descrição histopatológica e imunohistoquímica confirmaram diagnóstico do tumor de células ependimárias no IV ventrículo encefálico com metástase renal e pulmonar. Este tumor é considerado raro na espécie equina.

Background: Extramedullary plasmacytoma is a neoplasm originating from plasmacytes with benign behavior, although it can present malignant characteristics such as local invasion and metastases in some cases. Primary extramedullary plasmacytoma in the third eyelid is rare in humans and animals and has not yet been described in cats. Thus, the goal of the present study is to describe the cytological and histopathological findings of an extramedullary plasmacytoma in a cat's third eyelid.Case: A 5-year-old female feline patient with a history of ocular alteration for approximately 30 days was referred to the ophthalmology service. Upon ophthalmic examination of the left eye, an increase in volume with tissue proliferation was identified in the third eyelid's conjunctiva. A collection of material for cytopathological examination was performed. Numerous plasmocytes and atypical lymphocytes with anisocytosis and anisokaryosis were observed, with a possible neoplastic injury being suggested and referred to the patient for surgical removal of the third eyelid. In the histopathological analysis, it was possible to observe several plasma cells and vascular neoformation along the entire conjunctive margin below the epithelium. The glands, serous and mucous, present in the fragment, revealed areas of intervening plasmacytic infiltrate and a large area close to the hyaline cartilage containing intense plasmacytic inflammatory infiltrate with rare neutrophils and macrophages. There was also a structural breakdown of glandular components and lymphocyte cells with mitosis figures and discrete cellular pleomorphism.Discussion: Plasmacytoma is a rare neoplasm in both dogs and cats. The anatomical regions most frequently affected are the digits, lips, and pinna. Concerning the 3rd eyelid tumor involvement, numerous types of neoplasms have been reported in dogs in the veterinary literature, including transmissible venereal tumor, squamous cell carcinoma, and melanocytoma melanoma, lymphoma, plasmacytoma, hemangioma, hemangiosarcoma, mastocytoma, and myoepithelioma. In cats, the third eyelid is the third site most affected by primary tumors. Adult and elderly animals are the most affected; however, an extramedullary plasmacytoma (PEM) has been reported in an eight-month-old male cat's skeletal muscle. Plasmacytoma aspirates, like other round cells, tend to be highly cellular, the cell morphology has a plasmacytoid aspect, resembling mature cells, the cytoplasm is intensely basophilic, and the round nucleus with an eccentric disposition has thin chromatin and indistinct nucleolus. Binucleate and multinucleate cells are common, and the absence of lymphoglandular corpuscles helps differentiate extramedullary plasmacytoma from lymphomas. Histologically, PEM is similar in dogs, humans, and felines, and variations in mature and immature plasma cells can be seen. The diagnosis of extramedullary plasmacytoma is fundamentally histological, and immunohistochemical analysis can also be performed. Surgical excision with safety margins is the primary type of treatment for neoplasms in the 3rd eyelid, being considered an effective method, as seen in this report, during the patient's follow-up for 13 months, no recurrence or metastasis of the neoplasm was seen. When signs of malignant neoplasms are found, it is recommended to evaluate the indication for chemotherapy before surgery and monitor the case after treatment due to recurrence risk.

ResumoObjetivou-se relatar um caso de linfoma cutâneo não-epiteliotrópico em canino, destacando-se os aspectos clínicos e patológicos da neoplasia. Foi atendido no Hospital Veterinário da Universidade Federal Rural de Pernambuco (UFRPE), um cão de raça Shih-Tzu, macho, não castrado, com sete anos e pesando aproximadamente 5,3kg. O paciente apresentava neoformação ulcerada, eritematosa e com secreção purulenta em formato de anel em região do cotovelo no membro anterior direito e nódulo ulcerado e com presença de crostas em região do membro posterior esquerdo. Foram realizadas análises hematológicas, bioquímica sérica, citologia aspirativa por agulha fina e biópsia para análise histopatológica. No hemograma, detectou-se anemia normocítica normocrômica com leucocitose e neutrofilia relativa e absoluta. Ao exame bioquímico, observouse a presença de hiperproteinemia e hiperglobulinemia. A citologia demonstrou alta celularidade, com presença de células multinucleadas e figura de mitose. No exame histopatológico foi observada acentuada celularidade composta por células redondas, infiltrados neoplásicos dispostos em cordões localizados na derme profunda e tecido subcutâneo, sendo então, estabelecido o diagnóstico de linfoma cutâneo não epiteliotrópico. Por se tratar de uma enfermidade sistêmica, o linfoma torna-se uma neoplasia de difícil tratamento. O diagnóstico pela citologia aspirativa juntamente com o exame histopatológico e sinais clínicos foram essenciais para o diagnóstico de Linfoma não-epiteliotrópico no presente relato.Palavras-chave: canino; doença linfoproliferativa; epiderme; neoplasia. AbstractThe objective of this study was to report a case of nonepitheliotropic cutaneous lymphoma in a dog, highlighting the clinical and pathological aspects of this neoplasia. A seven-year-old intact male Shih-Tzu dog, weighing approximately 5.3kg was seen at the Veterinary Hospital of the Federal Rural University of Pernambuco (UFRPE). The patient presented an ulcerated, erythematous neoformation with purulent secretion in a ring-shaped region of the elbow in the right anterior limb and ulcerated nodule with crusts in the left hind limb. Hematological analysis, serum biochemistry, fine needle aspiration cytology, and biopsy for histopathological analysis were performed. Normocytic normochromic anemia was detected with leukocytosis and relative and absolute neutrophilia on blood count. Hyperproteinemia and hyperglobulinemia was observed on the biochemical exam. Cytology showed a high cellularity, with the presence of multinucleated cells and mitosis. Histopathological examination showed a marked cellularity composed of round cells, neoplastic infiltrates arranged in cords located in the deep dermis and subcutaneous tissue, and the diagnosis of nonepitheliotropic cutaneous lymphoma was established. Because it is a systemic disease, lymphoma becomes a difficult neoplasm to treat. Diagnosis by aspiration cytology together with histopathological examination and clinical signs were essential for the diagnosis of non-epitheliotr...

RESUMO: O Carcinoma de Células Escamosas é um tumor maligno dos queratinócitos. No equino representa de 6-10% de todas as neoplasias que afetam a genitália externa. Os sinais geralmente incluem placas despigmentadas, irregularidades da superfície do pênis ou prepúcio, erosões incuráveis com ou sem tecido de granulação acompanhado e casos de metástase são observados em aproximadamente 18% das ocorrências. Microscopicamente observa-se formação de pérolas de queratina, mitoses e atipia celular. O diagnóstico é feito a partir dos sinais clínicos, achados histopatológicos, ultrassonografia e punção aspirativa. Relata-se um caso de um equino, macho, castrado, 13 anos, SRD, pelagem alazão, que foi atendido no Hospital Veterinário do Campus Palotina da Universidade Federal do Paraná. Devido ao mau estado corporal o animal foi submetido à eutanásia, sendo realizada a necropsia e exame histopatológico dos órgãos que foram corados pela coloração de Hematoxilina e Eosina. Na necropsia foi observada uma massa irregular ulcerada, firme, branco-amarelada e com superfície de aspecto granular no pênis e prepúcio. Os pulmões indicaram presença de metástase. No histopatológico confirmou-se que as lesões eram metástases do carcinoma de células escamosas. O objetivo deste trabalho é relatar um caso de carcinoma de células escamosas em pênis e prepúcio de equino com metástase pulmonar. Palavras-chave: histopatológico, neoplasia, pênisABSTRACT: Squamous Cell Carcinoma is a malignant tumor of keratinocytes. In the horse represents 6-10% of all cancers affecting the external genitalia. The signals typically include unpigmented plaques, surface irregularities of the penis or the prepuce, incurable with or without erosions accompanied by granulation tissue and cases of metastasis are observed in approximately 18% of cases. Microscopically observed formation of keratin, mitoses and cellular atypia. Diagnosis is made from clinical signs, histopathological findings, ultrasound and needle aspiration. We report a case of a horse, male, neutered, 13 years, SRD, sorrel coat, which was the Veterinary Hospital of Palotina Campus of Federal University of Paraná. Due to poor body condition the animal was euthanized, necropsy and histopathological examination of organs which were stained with hematoxylin and eosin staining being held. At necropsy an ulcerated, firm, white-yellowish and granular appearance on the surface of the penis and foreskin irregular mass was observed. The lungs showed metastasis. In the histopathological it was confirmed that the lesions were metastases, squamous cell carcinoma. The objective of this study is to report a case of squamous cell carcinoma of the penis and foreskin in equine pulmonary metastasis.

ResumoObjetivou-se com este trabalho relatar um caso de linfoma imunoblástico em uma cadela diagnosticado pelo exame citopatológico. Analisou-se um animal da espécie canina, fêmea, sem raça definida, cinco anos de idade, procedente do Hospital Veterinário da Universidade Federal Rural de Pernambuco. De acordo com o histórico, o animal apresentou há aproximadamente três meses edema submandibular e em membros pélvicos que persistiam, com posterior aparecimento de nodulações nestas regiões, além de emagrecimento progressivo. Ao exame clínico foi constatado o aumento generalizado dos linfonodos superficiais e severa hipertrofia dos linfonodos submandibulares. Realizou-se a coleta de amostras dos linfonodos superficiais pela técnica de Citologia Aspirativa por Agulha Fina (CAAF), para diagnóstico citopatológico. A análise microscópica da amostra obtida pela CAAF revelou alta celularidade, presença de população de células de perfil linfocitário, compatíveis com imunoblastos, apresentando diâmetro nuclear variando entre uma e meia a duas hemácias, cromatina vesicular e nucléolo proeminente e central, citoplasma de basofilia leve a moderada. Foram observados também mitoses atípicas, acentuado pleomorfismo celular, anisocitose e anisocariose. Neste relato o linfoma foi anatomicamente classificado em multicêntrico. As alterações descritas ao exame citopatológico associadas ao histórico clínico do animal são compatíveis com linfoma imunoblástico de alto grau, sendo este o primeiro registro em um cão no estado de Pernambuco. A classificação citomorfológica associada a outros parâmetros pode propiciar ao médico veterinário um adequado direcionamento terapêutico, bem como auxiliar no estabelecimento do prognóstico.Palavras-Chave: canino; citopatologia; doenças linfoproliferativas; neoplasia. AbstractThe purpose of this study was to report a case of immunoblastic lymphoma in a dog diagnosed via cytopathology. A female, mix breed, five-year-old canine was seen at the Veterinary Hospital of the Federal Rural University of Pernambuco, Brazil. According to the history, the animal presented about three months prior with submandibular and pelvic limbs edema that persisted, with later appearance of nodulations in these regions, in addition to progressive weight loss. Clinical examination revealed a generalized increase in superficial lymph nodes and severe hypertrophy of submandibular lymph nodes. Samples of the superficial lymph nodes were collected using Fine Needle Aspiration Cytology (CAAF) for cytopathological diagnosis. The microscopic analysis of the sample obtained by CAAF revealed high cellularity, presence of lymphocytic cell population compatible with immunoblasts, with nuclear diameter ranging from one and a half to two red blood cells, vesicular chromatin and prominent and central nucleolus, cytoplasm of mild to moderate basophilia. Atypical mitoses, marked cellular pleomorphism, anisocytosis and anisokaryosis were also observed. In this report, the lymphoma was anatomically classified as multicentric. The changes descri...