Background: Gastroesophageal intussusception is characterized by the invagination of the stomach into the esophagus, with or without the involvementof adjacent organs such as the spleen, pancreas, and omentum. In dogs, this condition has no breed or sex predisposition. As it is an infrequent disease in routine veterinary medical practice, this study reports a case of gastroesophageal intussusception in a dog necropsied at the Veterinary Hospital of the Federal Rural University of Pernambuco (HOVET - UFRPE), Recife, Brazil.Case: The body of a 12-year-old black mixed breed male dog was sent to the Pathology Department (Necropsy Sector of the Federal Rural University of Pernambuco - UFRPE) for a necropsy. The animal had a previous 4-year history of recurrent emesis and limb weakness, primarily in the anterior limbs, that worsened in the previous months and progressed to death. No previous treatments were reported by the owner. On external examination, the animal had a low body score (cachectic), forelimb joints with great flexibility, congested oral and ocular mucous membranes, enophthalmos, and increased volume in the perianal region. At the opening of the thoracic cavity, the final third of the esophagus was dilated and gastroesophageal intussusception, edema, and pulmonary congestion were noted. In the abdominal cavity, there was hepatic and renal congestion and large intestine and rectal ampoule dilation, with a large amount of solid and retained feces (fecaloma), perianal hernia, and testicular neoformation. These findings were consistent with those observed in death caused by cardiorespiratory failure secondary to gastroesophageal intussusception.Discussion: The pathophysiology of gastroesophageal intussusception is still not elucidated and is probably multifactorial. This condition causes reverse gastric peristalsis associated with a sudden and sustained increase in abdominal pressure. Some probable predisposing factors for this pathological condition are esophageal motility disorders, lower esophageal sphincter dysfunction, and hiatal enlargement. In dogs, gastroesophageal intussusception is associated with increased intra-abdominal pressure owing to emesis or blunt trauma, negative intrathoracic pressure caused by respiratory, and previous esophageal diseases, especially megaesophagus. Partial or total obstruction caused by intussusception leads to circulatory disorders in the organs, especially decreased venous return. Persistence of this condition can lead to gastric necrosis and rupture followed by endotoxic (or septic) shock and release of inflammatory mediators that can cause cardiovascular and respiratory dysfunction and rapid death. Impaired circulation is macroscopically evident in several organs, characterized by mucosal, lung, liver, and kidney congestion, in addition to cardiac dilation and mitral valve endocardiosis. The occurrence of stomach invagination into the esophagus dilated in the final portion is characteristic of gastroesophageal intussusception. Moreover, death owing to cardiorespiratory failure is related to cardiac (dilatation and endocardiosis) and pulmonary (edema and congestion) involvement secondary to gastroesophageal intussusception. Since thispotentially fatal condition has a low incidence in small animals and often goes unnoticed by professionals, early and correct diagnosis along with surgical treatment are essential for a good prognosis and favorable progression.

RESUMOEste artigo objetiva apresentar o estado da arte relativo às aplicações ergonômicas em atividades florestais abrangendo os fatores humanos -o trabalho deve transcorrer sem monotonias e fadigas, com motivação e satisfação; à antropometria dos trabalhadores e o posto de trabalho -especificidade dentre o biótipo dos trabalhos (análise do perfil dos funcionários) com as atividades que precisam desempenhar; às condições do ambiente de trabalho (conforto térmico, ventilação, iluminância e ruído) -promover características ambientais adequadas para a realização das tarefas onde a energia dispendida durante a jornada de trabalho seja convertida em trabalho propriamente dito, com segurança e produtividade; e o layout do posto de trabalho -melhor acomodação de pessoas, máquinas e informação para que o trabalho discorra em um fluxo contínuo e constante, sem interrupções e gargalos. PALAVRAS-CHAVE: Antropometria; ambiente de trabalho, fatores humanos. ERGONOMICS IN FORESTRY ACTIVITIES ABSTRACTThe goal of this paper aims to present the state of the art concerning ergonomic applications in forestry activities encompassing human factors -work must elapse without monotony and fatigue, with motivation and satisfaction; anthropometry of workers and the workplace -specificity among the biotype of work (analysis of the worker profile) with the activities that need to be performed; conditions of the work environment (thermal comfort, ventilation, noise and illuminance) -to promote appropriate to carry out the tasks environmental characteristics where the energy expended during the workday to be converted into real work said, with safety and productivity, and the layout of the workplace -better conformation of people, machines and information for the job happens in a continuous and constant flow without interruptions and bottlenecks.

Background: Cryptococcosis is an important zoonotic disease that occurs worldwide. The disease is caused by a soilborne opportunistic fungus of the genus Cryptococcus, which can also be found in the feces of birds, especially pigeons. In Brazil, the geographical distribution of the agent is fairly defined, with the species C. gattii predominantly found in thenorth-eastern states. Diagnosis is based on the clinical history, physical examination findings, and results of complementary testing, such as cytopathological and histopathological examinations. This report aims to describe the clinical and anatomopathological findings in a case of systemic cryptococcosis in a dog.Case: A 4-year-old female Miniature Schnauzer was referred for necropsy. For 3 months prior to death, the dog had displayed generalized alopecia, pruritus, and severe weight loss, Initially, a private veterinarian had suspected leishmaniasis. Gross examination was performed and samples for cytopathology, histopathology, and fungal culture were obtained. Themacroscopic examination revealed generalized alopecia, congested mucosa, cachexia, hyperemia of the brain, and multiple white nodules measuring 0.5 cm to 4 cm in the lungs and the kidneys. None of the other organs showed significant lesions. Cytopathological examination of the nodules revealed a highly cellular sample, with a severe chronic inflammatory response, characterized by multinucleated giant cells and round-to-elliptical, yeast-shaped structures (5-10 μm), suggestive ofCryptococcus organisms. Histopathological examination of the lungs, kidneys, and brain revealed a severe diffuse chronic inflammatory process, with lymphocytic infiltration and multinucleated giant cells; countless yeast-shaped, round-to-ovoid structures (similar in appearance to “soap bubbles”) that were negative in hematoxylin-eosin stain were also present.Grocott’s methenamine silver stain was then applied, which positively stained the organism capsules black, confirming Cryptococcus. For mycological diagnosis, samples from the lungs nodules were cultured on Sabouraud’s dextrose agar supplemented with chloramphenicol 0.4% and incubated for 7 days at 25-30ºC. Abundant small, smooth, irregular-sized,cream-colored colonies were obtained, and a mycological smear, stained by India ink, was performed.Discussion: Considered an uncommon disease, canine cryptococcosis can be misdiagnosed. Clinical findings, such as alopecia, lymphadenopathy, and cutaneous nodules that may be ulcerated, are commonly seen in these patients due to the hematogenous spread of the organism. In the case described, the dog’s owner was contacted and asked to provide epidemiological information. The owner reported that the dog had lived in an apartment, with little access to the street; however, pigeons were frequently observed on the balcony, along with pigeon feces in the dog feeder. The presence of pigeon feces is a risk factor for the development and spread of the fungus, which may be problematic to public health. Cytopathological examination is a simple, quick diagnostic tool with good sensitivity for some infectious agents. Mycological culture can provide results due to the characteristic of the Cryptococcus sp., since the encapsulated yeast produces a mucous-like colony. Cryptococcosis has a relevant role in public health, so a precise diagnosis is imperative. Its diagnosis is based upon culture, cytology and serological tools. When the animal does not survive to the ilness, the association between necropsyfindings and histopathological examinations is essencial.Keywords: canine, diagnostic, yeast, anatomopathology.

Background: The chemodectoma is a rare neoplasm that originates from chemoreceptors located mainly in the aortic body, and carotid body and sinus, and responsible for detecting variations in blood pH, oxygen pressure and carbon dioxide. Dogs of brachiocephalic breeds and aged between 7 and 15 years have greater propensity. A neoplasm involves infiltrative growth in the vessels at the heart base, which leads to Congestive Heart Failure (CHF). The definitive diagnosis is performed by histopathological and/or immunohistochemical examination. The aim was to report a case of chemiodectoma in a dog, showed the disease clinical characteristics.Case: A 13-year-old male undefined breed dog was examinated in the medical clinic of small animal of Veterinary Hospital, Federal Rural University of Pernambuco (UFRPE) with respiratory effort, hyporexia, and edema in face, cervical, ventral thorax and thoracic limbs, with thirty days evoluted. About physical examination, we observed cachexia, orthopneic position, cyanosis and edema with a positive Godet sign, as well as 8% dehydration degree. Thoracic auscultation presented mixed dyspnea and muffled heart sounds. Chest radiography detected an radiopacity increase in pulmonary section and metastatic neoplastic process associated with pleural and pericardial effusion. Fluid therapy with lactated ringer and posterior thoracentesis in the right hemithorax region was performed for greater respiratory comfort for the patient. Hematological count and biochemical profiles stated normochromic normocytic anemia, relative and absolute lymphopenia, thrombocytopenia, as well as increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The patient died after 12 hours when was submitted to necroscopic examination and histopathological evaluation. An infiltrative tumor of cardiac base was observed invading the light of the right atrium, obstructing the venous return, as well as a large thrombus located in the left ventricle and diffuse nodules in the lungs. Hydrothorax and hydroperitoneum were observed with serous fluid, with hepatic, renal and brain congestion, and duodenum hemorrhage. Histologically, the tumor was characterized as a chemodectoma with pulmonary metastasis.Discussion: Cardiac tumors are uncommon for canine population. A study sampled 729,265 dogs with observed incidence reaching 0.19%. The most common type is hemangiosarcoma with 69%, followed by chemiodectoma and lymphoma. Authors describe predisposed brachycephalic breeds such as Boxer, Boston Terrier and French Bulldog. In our case, the patient had elongated snout and undefined breed. Deregulation of chemoreceptors, which detect changes in pH and partial oxygen and carbon dioxide pressures, can result in hyperpnea and dyspnoea. Edema represents a deficit in venous return from the cranial and cervical regions, with consequent increase in hydrostatic pressure and liquid leakage into the thoracic cavity and subcutaneous tissue, thus inferring the Caudal Vena Cava Syndrome (CVCS). Tumors from the cardiac base, integrated with the large arteries insertion and adjacent to the atria, can cause pericardial effusion observed in this case. Chemodectomas are described as essentially benign tumors with low metastatic potential. In this case, pulmonary metastasis was detected. Surgical treatment is recommended when feasible. In this case, the patient had late veterinary care, in addition to the contraindication for surgery by the lung metastasis presence.

Este estudo analisou laudos de necropsia e registros fotográficos de primatas não humanos da Divisão de Patologia do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco (UFRPE), Brasil. Os dados patológicos de 60 primatas não humanos (PNH) necropsiados entre janeiro de 2017 e dezembro de 2018 foram analisados com base nos seguintes critérios: cidade e zona (urbana, periurbana e rural), origem (livre e cativeiro), história e suspeita de diagnóstico clínico, sexo, espécie, idade, estado de conservação do cadáver e achados anatomopatológicos macroscópicos. Os achados anatomopatológicos dos animais viáveis necropsiados (53,33%) foram analisados estatisticamente para valores absolutos e relativos. As principais causas de morte foram traumatismo (42%), parasitismo (5%), pneumonia (3%) e distocia fetal (3%). O trauma, possivelmente causado pela antropização, foi a causa mais prevalente da morte dos primatas. Esta conclusão apresenta um importante diagnóstico diferencial na evolução da morte de primatas durante o período epizoótico.

A partir do ano de 2011, o médico veterinário foi integrado às equipes multiprofissionais do Núcleo de Apoio à Saúde da Família (NASF), denominação esta da Portaria MS 2.488/2011, alterada para Núcleo Ampliado de Saúde da Família e Atenção Básica (Nasf-AB) pela Portaria Ms 2.436/2017, apesar de ser considerado como profissional da saúde pelo Conselho Nacional de Saúde (CNS) desde 1988 pela resolução CNS 287/1998. O médico veterinário atua principalmente em epidemiologia e formas de prevenção e controle das enfermidades causadas por alimentos de origem animal em humanos, além do controle e prevenção das zoonoses para promoção da saúde humana. Objetivou-se relatar a vivência dos médicos veterinários residentes, da Universidade Federal Rural de Pernambuco, durante sua atuação no (Nasf-AB), do município de Jaboatão dos Guararapes, Pernambuco, Brasil, no período de Outubro a Dezembro de 2017. Durante o período da vivência foram realizadas várias atividades, como visitas domiciliares, matriciamento, atividades de educação em saúde em sala de espera, e participação em reuniões sobre discussão de casos. Este relato demonstra a importância da execução das atividades práticas realizadas no Sistema Único de Saúde (SUS), durante o programa de residência na área profissional da saúde em Medicina Veterinária.

Histiocytic sarcoma (HS) refers to a rare neoplasm of round cells frequently presented in a disseminated form, affecting bones, joints, skin, and subcutaneous tissue. The objective of this study was to report a case of disseminated HS in a canine attended at HOVET-UFRPE, Recife, Brazil. Physical examination and complementary tests were performed; complete blood count and serum biochemistry were normal, but at thoracic radiography exam a neoformation was detected in the right lung. Cytopathological evaluation was suggestive of malignant epithelial neoplasia. At necropsy, there were disseminated nodules in the lungs, liver, spleen, kidneys, meninges and eye. The histopathological examination revealed proliferation of pleomorphic round cells, suggesting HS, which was confirmed by immunohistochemistry. From the obtained results, it becomes evident the importance of complementary exams to properly diagnose histiocytic diseases. This is one of the few reports of canine disseminated HS in Brazil.

Background: Lymphoma is a neoplasm of hematopoietic origin that affects canines. The proper establishment of prognosis and rapid institution of treatment are essential for a better quality of life, and immunophenotyping is one of the tools used for this purpose. The objective of this study was to perform a clonality test for immunophenotypic characterization of canine lymphomas using the polymerase chain reaction (PCR) for antigen receptor rearrangements (PARR) technique in real-time from samples fixed in formalin and embedded in paraffin.Materials, Methods & Results: The 23 analyzed samples were fixed in formalin and embedded in paraffin canine lymphoma from the Collection Laboratory of Histopathology of the Animal Pathology Area of the Departament of Veterinary Medicine - Federal Rural University of Pernambuco UFRPE. Samples were processed, their DNA was extracted, quantified, diluted, and standardized at a concentration of 50 ng/µL. After extraction, all samples were subjected to conventional PCR for endogenous control (detection of the IgM target region), in which the extracted DNA was amplified in a final volume of 25 µL. The 128 bp amplified product was detected by 1.5% agarose gel electrophoresis. Of the 23 samples analyzed for the detection of the conserved region referring to the endogenous gene, 91.30% (21/23) amplified the conserved region Cµ by conventional PCR, and two samples 8.70% (2/23) were negative. Endogenous control positive samples were subjected to real-time PCR-PARR for detection of IgH Major (LB), IgH Minor (LB), and TCRγ (LT) target regions. All reactions were performed in duplicate to reduce the risk of false-positive or false-negative results due to technical errors. Samples previously confirmed by immunohistochemistry were used as positive controls for T cell and B cell lymphoma, and MilliQ water was used as a negative control. After amplification, the melting curve gradually increased the temperature by 1C/5 s to 95C during continuous fluorescence monitoring. Of the 21 samples analyzed, 100.00% (21/21) demonstrated clonal amplification. Of these, 57.15% (12/21) were positive for phenotype B, and 42.85% (9/21) were positive for phenotype T.Discussion: Due to the importance of researching and confirming samples from files fixed and embedded in paraffin samples in laboratories, PCR-PARR is a good tool for this purpose. In the present study, real-time PCR analysis demonstrated greater sensitivity in the characterization of the immunophenotype of lymphomas from old samples fixed in formalin and embedded in paraffin. The temperature of melting curve analysismay vary depending on the amount of DNA and its quality. In the present study, it was found that the average melting temperature in the samples varied between ± 3C when compared to that in the control sample for LB and LT, 83.5C and 80C, respectively: in the literature, there is a relative difference in this temperature, which may vary up to 4C. Real-time PCR-PARR was satisfactory in the characterization of the immunophenotype of canine lymphomas from formalin-fixed and paraffin-embedded samples; therefore, its use is recommended for both retrospective studies. The use of PCR-PARR associated with histopathological and/or cytopathological examination in cases of canine lymphomas strongly helps pathologists, provide a safe establishment of the immunophenotype, minimize errors, and optimize the diagnosis, thus directly contributing to the establishment of the prognosis.Keywords: immunophenotyping, lymphoproliferative disease, real-time PCR, TCRγ, IgH.