Application volume, adjuvants that act on the drop surface tension and droplet spectrum are the main factors affecting leaf surface coverage and the spray penetration droplets inside the canopy of the soybean crop, which may have an impact on fungicide efficiency, since those used in the handling of Asian-rust, including those that are absorbed by the plant, present a small displacement from the point of deposition. Thus, it was sought to determine the impacts on the use of the organosilicone surfactant compared to the changes in the spray volumes. For that, five spray volumes and the combination of two adjuvants were compared in randomized blocks with a factorial arrangement 5x2. Reductions in volume negatively impacted the number of drops cm-2 affecting the leaf surface cover which did not exceed 13% in the lower third, in the absence of the surfactant. Also, the reduction in the volume provided decreases in the control of Asian-rust, and the use of surfactant minimized the negative impacts of the disease, reflecting on the index of the leaf area which varied up to 6.5 times on changes in the spray volume, and up to 2.8 times against the use of the surfactant in relation to the mineral oil isolated. Grain yield was negatively affected as reductions in the spray volumes, occurred with less impact when the surfactant was used. Thus, coverage of the leaf surface and the number of drops cm-2 along the plant profile respond to the addition of the surfactant and to the increase of the spray volume, having relation with the control of soybean Asian-rust, and affecting the leaf area index and productivity.

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The purpose of this study was to check the influence of leaf area index, spray volumes and applicable quantities of fungicide during the protection period, and for control of Asian soybean rust. All tests were conducted in the field and in the laboratory between 2014 and 2016. The quantity of fungicide and the spray volume were varied in the application of fungicides on soybean plants with different leaf area index. Rainfall simulation tests were also carried out at different times after application of fungicide. The spray volume and application rate of fungicide should be adequately proportioned according to the leaf area index of the soybean cultivar. The closer to the time of application that the rain occurs, the lower the control obtained by applying the fungicide. The leaf area index, the fungicide application rates, and the spray volumes influenced the protection period of fungicides and the control of Asian soybean rust.

Asian soybean rust is one of the most destructive diseases that can be found in this crop. It can be largely controlled by fungicide application. The objective was to assess the sensitivity of P. pachyrhizi isolates to fungicides. The tests were performed in a completely randomized design, with six replicates. The sensitivity of twelve isolates to site-specific and multisite fungicides at concentrations of 0.1; 1.0; 10.0, and 100.0 mg L-1, plus a control with absence of fungicide (0.0 mg L-1) was assessed. Soybean leaflets were immersed in the appropriate fungicide solutions, disposed in wet chambers in plastic boxes, and inoculated using each uredinia suspension of P. pachyhrizi (5.0 x 104 uredospores mL-1), separately. Boxes were incubated for 20 days at a temperature of 23°C and a 12-hour photoperiod. Next, the number of uredinia per cm2 on the abaxial face of each leaflet was evaluated. The active ingredients prothioconazole, trifloxystrobin, fluxapiroxade, trifloxystrobin + prothioconazole, trifloxystrobin + bixafen + prothioconazole, azoxystrobin + benzovindiflupyr, and azoxystrobin + benzovindiflupyr + diphenoconazole were highly fungitoxic for the majority of the isolates, with EC50 lower than 1.0 mg L-1. Diphenoconazole, azoxystrobin, and fenpropimorph were considered moderately fungitoxic for nine of the twelve isolates, with EC50 between 1 and 10 mg L-1. The multisites mancozeb and copper oxychloride presented EC50 responses classified as low toxic for the twelve isolates and eight for chlorothalonil (EC50 between 10 mg L-1 and 50 mg L-1). Site-specific fungicides showed high-to-moderate fungitoxicity to P. pachyrhizi isolates, even as the multisites presented moderate-to-less toxic activity.

The objective of this study was to evaluate genetic stability by determining the meiotic index (MI) and pollen viability in F1RC2 segregating populations, obtained by the crosses between wheat commercial cultivars and synthetic wheat, aimed at introgression/genetic resistance. For MI, three ears were collected per genotype, before anthesis, and they were fixed in Carnoy’s solution. The cytological slides were prepared by crushing the anthers and staining with 1% acetocarmine, and the normal tetrads and the ones with micronuclei were observed under a light microscope. For pollen viability, the ears were collected at the stage of mature pollen grain. The methodological procedure was similar to that of the tetrads, evaluating the pollen grains as viable, with little starch, with two pores, different sizes and non-viable. The pollen grain size was measured by the Axion Vision software. All the segregating populations with MI above 90% and high pollen viability (above 85%) reflected a stable meiotic behavior. About the variation in size, the CIGM90.909/BRS 179 cross showed pollen grains with 58.43 μm and CIGM93.298/BRS Guamirim, 47.15 μm. According to the results, wheat segregating populations were considered meiotically stable and suitable for use in wheat breeding programs and for incorporation of new important genes.

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