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We investigated the impact of haplotype of major histocompatibility complex (MHC)-B on the outcome of infection of Synthetic Dam Line (SDL) broiler strain with Rous Sarcoma Virus (RSV). Genomic analysis of MHC-B haplotypes, revealed a total of 12 known standard haplotypes that constituted to twenty-five different genotypes and one new haplotype of 217 bp size, designated B. The inoculation of RSV-A in SDL chicks resulted in the development of tumors of progressive or regressive phenotypes with varying tumor profile index (TPI). Haplotypes B, B and Bhad low TPI scores (1 or 2) with less mortality and were resistant to RSV-A tumor. The haplotypes B, B, B, Band Bhad significantly higher TPI scores (5 or 6), indicating a susceptibility to RSV-A. The genotype, B /B, had a mean TPI score of 3.67 ± 1.33, which was closer to the resistant haplotype. Sequence analysis of the new haplotype (B) revealed 99.5% similarity with B2 haplotype. Metastases was observed in 44% of chicks and comprised of mixed fibrosarcoma and myxosarcoma.

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The present experiment was carried out to study the association of milk quality traits (fat, protein, lactose, solid not fat (SNF), total solids (TS) and somatic cell count (SCC)) with teat traits ((teat length (TL), teat diameter (TD), distance between teats (DbT) and teat tip height from ground (THFG)) and udder traits ((udder circumference= UC, udder depth (UD), udder width (UW) and udder height from ground (UHFG)), in Tharparkar cows. The experiment was carried out on 32 tharparkar cows of different parities and lactation stages. Morning and evening measurement of teat and udder traits was carried out before milking and approximately 30 ml of representative milk samples were taken from each of the functional quarters and one composite milk sample of each experimental animal fortnightly from the date of calving of the animal in clean sampling bottles. Fat% and SNF% showed a negative, significant (P>0.05) to highly significant (P>0.01) correlation with TD, UL and FUD. Protein% expressed a positive, significant (P>0.05) to highly significant (P>0.01) correlation with UC, FUD and DbT. Further, Fat%, SNF% and TS% expressed a positive, significant (P>0.05) to highly significant (P>0.01) correlation with THFG. SCC (×105/ml) showed a positive, significant (P>0.05) to highly significant (P>0.01) correlation with TL, TD, DbT, UC and UL, whereas it had a negative correlation with THFG, rear-udder depth (RUD) and UW. It was further observed that Fat% expressed a positive highly significant (P>0.01) phenotypic correlation with SNF% and TS%. SNF%, Lactose%, Protein% and TS% expressed a negative phenotypic correlation with SCC.

Paswan, C.. 2005. Relationship between the bovine major histocompatibility complex (BOLA) with somatic cell count in crossbred cattle. J. Appl. Anim. Res., 28: 129-132.Characterization ofBoLA-DRB 3.2 gene was carried out in 25 crossbred cows suffering from clinical mastitis and 50 apparently healthy cows by PCR-RFLP. Digestion of 284 bp product of DRB3.2 gene with HaeIII restriction enzyme revealed five patterns out of these HaeIII-a (0.53) and -ah (0.38) being more frequent. RsaIn (0.120) pattern with highest frequency was seen in the cows of both groups. However, the patterns HaeIIIe, -ale, RsaI-b, -k, -1, -aD, -d/s, -g/n, -l/n, -110 and -m/n were observed only in apparently healthy cows and RsaIf, -0, -ch, glo and nlspatterns only in the cows suffering from clinical mastitis. PstI restriction enzyme did not reveal any restriction site in crossbred cows. The somatic cell count (SCC) (5519401 2787818) and somatic cell score (SCS) (6.6620.05) were found significantly higher in milk of the cows suffering from clinical mastitis as compared to those of apparently healthy group. The results showed non-significant effect ofHaeIII and RsaI patterns lgenotypes of BOLA DRB3.2 alleles on SCC and SCS in crossbred cows.