IDENTIFICAÇÃO DE FORMIGAS ASSOCIADAS À Staphylococcus spp. EM DIFERENTES AMBIENTES _______________________________________________________ RESUMO Este estudo teve como objetivo avaliar formigas associadas à Staphylococcus spp. em hospital, área comercial e ambiente domiciliar. Dez formigas foram coletadas em cada ambiente em Três Corações-MG. A condução da pesquisa compreendeu o período de abril a junho de 2018. As formigas foram coletadas assepticamente com pinças estéreis e transferidas individualmente para tubos de ensaio contendo o meio Brain Heart Infusion Broth (BHI). Em seguida foram transportadas para laboratório e colocados na Estufa Incubadora à 37ºC e observados após 18 horas de incubação, sendo retirados após 24 horas e transferidos para a geladeira, onde ficaram por dois dias. Após, foi preparado o meio Manitol Salt Agar e foi distribuído nas placas de Petri. Logo, com o auxílio de pipetas individuais retirou-se 500 µL de BHI de cada tubo e espalhou em cada placa. As placas foram levadas para a Estufa por 48 horas. As formigas foram transferidas para tubos contendo álcool 70% e levadas ao Laboratório de Entomologia da UninCor para identificação. A identificação foi realizada com auxílio de microscópio estereoscópio e chaves de identificação específicas para formiga. Foram identificadas seis subfamílias e treze gêneros de formigas associadas à Staphylococcus spp. nos ambientes estudados. O gênero mais encontrado nos três ambientes foi Tapinoma spp. da subfamília Dolichoderinae. As espécies identificadas são comuns no ambiente hospitalar e urbano e normalmente estão relacionadas a problemas estruturais e/ ou de higiene. Conclui-se que as formigas apresentam presença de microbactérias nos ambientes pesquisados. Palavras-chave: Bactérias. Formicida. Tapinoma spp. ABSTRACT This study aimed to evaluate ants associated with Staphylococcus spp. in hospital, commercial area and home environment. Ten ants were collected in each environment in Três Corações-MG. The conduction of the research comprised the period from April to June 2018. The ants were collected aseptically with sterile tweezers and transferred individually to test tubes containing the Brain Heart Infusion Broth (BHI) medium, then transported to the laboratory and placed in the Greenhouse Incubator at 37ºC and observed after 18 hours of incubation, being removed after 24 hours and transferred to the refrigerator, where they stayed for two days. After, the Mannitol Salt Agar medium was prepared and distributed in Petri dishes. Then, with the aid of individual pipettes 500 μL of BHI was withdrawn from each tube and spread on each plate. The plates were taken to the greenhouse for 48 Revista da Universidade Vale do Rio Verde | v. 16 | nº. 3 | Edição Especial, 2018 | p. 2hours. Through the research it was identified that six subfamilies and thirteen genera of ants associated with Staphylococcus spp. in the studied environments. The species identified are common in the hospital and urban environment and are usually related to structural and / or hygie...
The study was conducted with the objective of comparing the toxicity and the effect of the combination of intra and extracellular cryoprotectants in curimba Prochilodus lineatus sperm cells subjected to cryopreservation. Semen from 19 males were analyzed and diluted in four solutions comprise of intra and extracellular cryoprotectants in the following combinations: methanol+lactose, methanol+egg yolk, DMSO+lactose and DMSO+egg yolk. A portion of the diluted semen was frozen while the remaining fraction was kept in repose and evaluated after 10 min. For freezing, the diluted samples were packaged in 0.50 mL straws and placed into liquid nitrogen vapor for 24 h and, after this time, submerged into liquid nitrogen for 10 days. The combination of DMSO+lactose was less toxic to the diluted semen, resulting in higher motility rates and durations when compared to the other treatments. After thawing, the highest motility rate and duration were obtained using lactose as extracellular cryoprotectant, regardless of its combination. There was no significant difference between treatments when analyzing sperm morphology after thawing. Considering the effects of the tested treatments, the use of lactose as an extracellular cryoprotectant added with DMSO or methanol is the most suitable, since these combinations presented the highest motility rates and durations and low morphological change rate after thawing.Keywords: genetic preservation; cryobiology; sperm analysis; freezing protocol; toxicity; curimba CRIOPRESERVAÇÃO DO SÊMEN DO Prochilodus lineatus: EFEITO DA COMBINAÇÃO DE CRIOPROTETORES RESUMOO estudo foi realizado com o objetivo de comparar a toxicidade e o efeito da combinação de crioprotetoresintra e extracelulares em espermatozóides de curimba Prochilodus lineatus submetidos à criopreservação. O sêmen de 19 machos foi analisado e diluído em quatro soluções com crioprotetores intra e extracelulares nas seguintes combinações: metanol+lactose, metanol+gema de ovo, DMSO+lactose e DMSO+gema de ovo. Uma porção do sêmen diluído foi congelada enquanto que a fração remanescente foi mantida em repouso e avaliada após 10 min. Para o congelamento, as amostras diluídas foram envasadas em palhetas de 0,50 mL e colocadas em vapor de nitrogênio líquido durante 24 h e, após este tempo, submersas em nitrogênio líquido durante 10 dias. A combinação de DMSO+lactose se mostrou menos tóxica para o sêmen diluído, resultando em taxas mais elevadas de motilidade e duração espermática quando comparadas com os outros tratamentos. Após descongelamento, as maiores taxas de motilidade e duração foram obtidas usando lactose como crioprotector extracelular, independentemente da sua combinação. Não houve diferença significativa entre os tratamentos ao analisar a morfologia espermática após a descongemento. Considerando-se os efeitos dos tratamentos utilizados, o uso de lactose como crioprotector extracelular, adicionada ao DMSO ou metanol, é o mais adequado, uma vez que estas combinações apresentaram maiores taxas de motilidade e duração espe...
The Brazilian freshwater fish diversity is the richest in the world. The majority this fish species migrate during the spawning season a phenomenon known as piracema . Urbanization, pollution, hydroelectric dams and deforestation are some of the causes of stock depletion or even local extinction of some of these species [ ].The disappearance of native fish species and the decrease of genetic stocks undertake the aquatic ecosystem and commercial food production. Cryopreservation of semen is a biotechnology that has the ability to minimize these problems, increasing flexibility and operationalization of the reproductive period and improving assisted reproduction programs in native fish [ , ]. This also enables the biotechnology application of new techniques such as transplantation of gametes to form chimeras [ ].May be cited as advantages of semen cryopreservation: i sincronyzation of the gametes release from both sexes, ii semen economy, iii easeness of the management with breeders, iv transport of gametes from different locations and v genebanks for genetic selection programs and species conservation [ ] and decreased need for males breeders from nature.However, the heterogeneity of semen responses after freezing has hampered the standardization of a protocol for the different species of fish [ ]. Protocols have been tested for Salminus
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