Anxiety-associated behavior and genotoxicity found in adult  Danio rerio  exposed to tebuconazole-based commercial product

Castro, Tássia Flávia Dias; Souza, José Gilmar da Silva; Carvalho, Aline Ferreira Souza de; Assis, Isadora de Lima; Palmieri, Marcel José; Andrade-Vieira, Larissa Fonseca; Marcussi, Silvana; Machado, M. R. F.; Murgas, Luís David Solis

doi:10.1016/j.etap.2018.06.011

Cited by 33 publications

(7 citation statements)

References 35 publications

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“…As a triazole fungicide, TEB has been widely used in agriculture, leading to adverse effects on humans and wildlife (Sivikova, Dianovsky, Holeckova, Galdikova, & Kolesarova, ; Renzo et al, 2011; Castro et al, ; Bernardes, Andrade‐Vieira, Aragao, Ferreira, & Ferreira, ; Mercadante et al, ). The persistence in the environment and long half‐life mean that transgenerational investigation by parental exposure becomes very significant.…”

Section: Discussionmentioning

confidence: 99%

Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans

Liu

2020

J of Applied Toxicology

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The transgenerational reproductive and developmental toxicity of tebuconazole (TEB) in Caenorhabditis elegans was investigated over five generations (P0 − F4). Only parental C.elegans (P0) were exposed to TEB (0, 0.01, 0.1, 1, and 10 μg/L) for 24 h and the subsequent offspring (F1-F4) were grown under TEB-free conditions. TEB exposure caused dose-dependent reproductive defects and developmental impairments in C.elegans. In the P0 generation reproductive defects were observed such as:reduced brood size and embryo hatchability, prolonged generation time, retarded gonadal development, and slower germline proliferation, even at 0.01 μg/L, together with developmental toxicity with significant reduced body length and narrowed body width at 10 μg/L. Additionally, the brood size significantly reduced in F2, which began to recover from F3, but was still lower than the control in F4. The proportion of abnormalities increased significantly in F2 and reduced from F3, but was still higher than the control, suggesting that TEB could have cumulative potential and be passed to offspring through parental exposure. Furthermore, exposure to TEB (10 μg/L) in P0 significantly reduced the body length in F1, which began to recover from F2, and was the same level as the control in F4. There was a concentrationdependent increase in body width in F1-F4, with a significant increase only observed in F1 at 10 μg/L. Thus, parental exposure to TEB induced transgenerational defects in both reproduction and development, emphasizing the significance of considering bio-toxicity over multiple generations to conduct accurate assessment of environmental risks of toxicants. K E Y W O R D SCaenorhabditis elegans, parental exposure, reproductive and developmental toxicity, Tebuconazole, trans-generation

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Section: Discussionmentioning

confidence: 99%

Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans

Liu

2020

J of Applied Toxicology

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“…Sobre el zebrafish también es importante destacar que, hoy en día, posee un papel de destaque en estudios que envuelven el monitoreo ambiental y la toxicidad de substancias como metales pesados, compuestos carcinogénicos y investigaciones genotóxicas (Castro et al, 2018).…”

Section: Tecnologia Bft En Países Latinoamericanosunclassified

Elaboração, Caracterização Nutricional E Microbiológica De Iogurtes Com Adição De Coco Queimado E Calda De Coco, Preparados a Partir De Polpa De Coco Verde Da Espécie (Cocus Nucifera L.)

Luz¹,

Oliveira²,

Mouchrek³

et al. 2020

As Ciências Agrárias E Seus Impactos Na Sociedade (Vol. 3)

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“…Apart from using zebrafish as an in vivo model system, in addition to the zebrafish embryo model that can be regarded as an in vitro method, the use of zebrafish cultured cell lines in genetic toxicology is also on the rise [91,[97][98][99][100][101]. Up to now there have been more than 100 papers describing the zebrafish model both in vivo and in vitro using the comet assay for the assessment of different physical and/or chemical agents such as gamma rays [102,103], X-rays [104], pesticides [105], insecticides [106], fungicides [88,107], herbicides [108], mycotoxins [109], pharmaceuticals [98,99,[110][111][112], heavy metals A c c e p t e d M a n u s c r i p t 9 [108,113,114], PAHs [89], nanoparticles [115][116][117][118], flame retardants [119], sewage effluent [120], waste material [121] as well as model toxicants such as benzo(a)pyrene (B[a]P), methyl methanesulfonate (MMS) and ethyl methanesulfonate (EMS) [122,123].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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Anxiety-associated behavior and genotoxicity found in adult Danio rerio exposed to tebuconazole-based commercial product

Cited by 33 publications

References 35 publications

Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans

Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans

Elaboração, Caracterização Nutricional E Microbiológica De Iogurtes Com Adição De Coco Queimado E Calda De Coco, Preparados a Partir De Polpa De Coco Verde Da Espécie (Cocus Nucifera L.)

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

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