Whole blood samples were collected from 117 male clinically healthy Camelus dromedarius aged between 6 months to 18 years from several farms in Yazd Province of Iran. Trypanosoma evansi-affected camels were detected by Giemsa-stained blood smears, and the positive blood samples (4 out of 117) were submitted to PCR examination and phylogenetic analysis. Basic Local Alignment Search Tool data of the obtained complete internal transcribed spacer (ITS) sequences revealed that they corresponded to those of T. evansi, Thailand cattle isolate (AY912276) with the homology of 99 %. Both phylogenetic trees generated by ITS1 and complete ITS were unable to clearly show inter- and intraspecific genetic diversity of Trypanosoma spp. isolates. The phylogenetic tree inferred from the ITS2 nucleotide sequences (569 bp) clearly showed the genetic diversity of the parasites. Phylogenetic and molecular analyses of this region showed that two distinct genotypes of T. evansi in Iranian dromedary camels are present. In contrast to the ITS1 and ITS2 regions, multiple alignment of the nucleotide sequence of the 5.8S rRNA showed a high degree of sequence conservation during evolution in various Trypanosoma spp.

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Information regarding normal electrocardiographic features of different breeds of animals can help veterinarians to detect any abnormalities in cardiac electrical activities. The current research was conducted to present the normal electrocardiographic indices of clinically healthy Chios ewes and lambs. The electrocardiograms were recorded from clinically healthy Chios ewes (n=27; 2-3 years old) and lambs (n=20; 4-6 months old) by using base apex lead system. T and QRS-duration in lambs were significantly lower than adult Chios ewes. The electrocardiographic amplitudes in lambs were lower than ewes, non-significantly. P-R, R-R, Q-T and S-T intervals in Chios lambs were significantly lower than ewes. No normal sinus rhythm was detected in Chios lambs. The proportion of sinus arrhythmia and sinus tachycardia in lambs was significantly more than ewes. Sino-atrial block was also detected in lambs. Based on the presented data it could be stated that aging can affect electrocardiographic findings of Chios sheep. Finally, our results will provide a good basis for judging the electrocardiograms in base apex lead system of Chios lambs and ewes.

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Information regarding metabolic profile in different physiological sates of dairy cows can assist veterinarians to monitor the herd health and productive performance. Furthermore, the relationships among the metabolic parameters can be used to detect the effect of each parameter on another one. The aim of the current research was to clarify the interactions among metabolic parameters in different physiological states of high producing Holstein dairy cows. The present study was carried out on 25 multiparous Holstein dairy cows divided into 5 equal groups: early, mid and late lactation; far-off and close-up dry. Blood samples were collected from all cows through jugular venipuncture and sera were separated to evaluate glucose, insulin, β-hydroxybutyric acid (BHBA), non-esterified fatty acids (NEFA), triglyceride, cholesterol, high-density lipoprotein, low-density lipoprotein and very lowdensity lipoprotein. There were negative and significant correlations between insulin and glucose in all studied groups (P<0.05). The correlation coefficients among insulin and both NEFA and BHBA in all cows were negative. In all studied groups, the negative correlations were seen among glucose and both NEFA and BHBA. In all studied groups, glucose and insulin were negatively correlated with lipid profile. The correlation between BHBA and NEFA was positive, strong and significant in all groups (P<0.05). In all studied groups, BHBA and NEFA were positively correlated with lipid profile but no significant correlations were seen among them (P>0.05). The correlations among studied metabolic parameters showed that providing the energy demands can prevent the ketogenic and lipolytic metabolisms in high producing dairy cows. Furthermore, information regarding the correlations among circulating metabolic parameters can be used to estimate the changing patterns of each metabolic parameter via evaluating another one.Dairy cows undergo metabolic changes during pregnancy and lactation (Tanritanir et al., 2009). Each state has metabolic characteristics which are different from those of others. Two to three weeks before parturition, a phase of catabolism starts to

The effective treatments of endotoxemia are necessary to prevent high mortality rates. Hence, the present study was performed to clarify the antiendotoxic effects of tyloxapol and pentoxifylline in experimentally induced endotoxemia in sheep. Thirty clinically healthy 1-year-old Iranian fat-tailed ewes were randomly divided into six equal experimental (n = 5) groups, comprising Negative and Positive control, Tyloxapol 1, Tyloxapol 2, Pentoxifylline 1 and Pentoxifylline 2. Phenol extracted lipopolysaccharide from Escherichia coli serotype O55:B5 was infused at 2 µg/kg intravenously. Tyloxapol (200 and 400 mg/kg) and pentoxifylline (30 and 60 mg/kg) were injected to Tyloxapol and Pentoxifylline groups, respectively, at 90 min after endotoxemia induction over 60 min along with intravenous fluids. Blood samples were collected from all ewes prior and 1.5, 3, 4.5, 6, 24 and 48 h after lipopolysaccharide injection and sera and plasmas were separated, subsequently. Haptoglobin, serum amyloid A, tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), superoxide dismutase and glutathione peroxidase were measured in all samples. Serum concentrations of haptoglobin, serum amyloid A, TNF-α and IFN-γ in Tyloxapol 1 and 2 and Pentoxifylline 1 and 2 groups were significantly lower than Positive control one after hour 3. There were no significant differences among Tyloxapol and Pentoxifylline groups (P > 0.05). Superoxide dismutase and glutathione peroxidase activities in Tyloxapol 1 and 2 and Pentoxifylline 1 and 2 groups were significantly lower than Positive control one after hour 3. There were no significant differences among Tyloxapol 1 and 2 and Pentoxifylline 1 and 2 groups (P > 0.05). Tyloxapol and pentoxifylline act as the anti-inflammatory mediators by decreasing pro-inflammatory cytokines and hepatic APPs and modulating oxidative enzymes activity after endotoxemia induction in sheep. Furthermore, their efficacies at different doses were significantly similar together and both drugs don't induce their effects by dose dependent manner and the anti- and pro-inflammatory effects of them were statistically similar.

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