Modulation of inflammatory responses following insulin therapy in experimentally bolus intravenous Escherichia coli lipopolysaccharide serotype O55:B5 induced endotoxemia in Iranian fat-tailed sheep

Chalmeh, Aliasghar; Badiei, Khalil; Pourjafar, Mehrdad; Nazifi, Saeed

doi:10.1016/j.smallrumres.2013.03.002

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…So, the photoperiod-dependent LPS effects observed in sheep may be of high interest and point to sheep as an interesting model in the research on the use of insulin in the states related to the occurrence of acute stress reactions, considering the natural leptin resistance state in this animal during the LD season. The potency of insulin usage in inflammation in sheep was studied by Chalmeh et al [ 41 ], who observed that the i.v. injection of insulin may have a potential anti-inflammatory effect in Iranian fat-tailed ewes with LPS-induced endotoxemia.…”

Section: Discussionmentioning

confidence: 99%

“…However, the authors conducted the experiment only in one season, April, which is a rather non-breeding season in the Badjgah region of Iran, with a semiarid climate [ 42 ], and should thus correspond to the ‘long-day sheep model’. However, Chalmeh et al [ 41 ] presented no results for the SD season (breeding season), in which the results could be significantly different. Assuming that the results obtained by Chalmeh et al [ 41 ] present the results obtained for LD sheep (a natural model for leptin resistance in obesity), it could be concluded that, in acute conditions, insulin should be administered to people with leptin resistance problems or obese people but not to healthy people.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Leptin on the Blood Hormonal Profile (Cortisol, Insulin, Thyroid Hormones) of the Ewe in Acute Inflammation in Two Different Photoperiodical Conditions

Krawczyńska

Herman

Antushevich

et al. 2022

IJMS

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As a day animal with sensitivity to inflammation similar to that of humans, the sheep may highly outperform the rodent model in inflammation studies. Additionally, seasonality makes sheep an interesting model in endocrinology research. Although there are studies concerning inflammation’s influence on leptin secretion and vice versa, a ewe model, with its possible ‘long-day leptin resistance’, is still not examined enough. The present study aimed to examine whether leptin may modulate an acute inflammation influence on plasma hormones in two photoperiodical conditions. The experiment was conducted on 48 ewes divided into four groups (control, lipopolysaccharide (LPS), leptin, LPS + leptin) during short and long days. Blood sampling started 1 hour before and continued 3 h after LPS/saline administration for further hormonal analysis. The results showed that the photoperiod is one of the main factors influencing the basal concentrations of several hormones with higher values of leptin, insulin and thyroid hormones during long days. Additionally, the acute inflammation effect on cortisol, insulin and thyroid hormones was photoperiod-dependent. The endotoxemia may also exert an influence on leptin concentration regardless of season. The effects of leptin alone on hormone blood concentrations are rather limited; however, leptin can modulate the LPS influence on insulin or thyroxine in a photoperiod-dependent way.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Effect of Leptin on the Blood Hormonal Profile (Cortisol, Insulin, Thyroid Hormones) of the Ewe in Acute Inflammation in Two Different Photoperiodical Conditions

Krawczyńska

Herman

Antushevich

et al. 2022

IJMS

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“…There were no significant differences between polymyxin B groups in terms of decreasing the concentrations of TNF-α. Increased serum concentration of TNF-α in response to endotoxaemia in sheep has been studied previously (6,24). Parviainen et al (17) used polymyxin B in treatment of experimentally-induced endotoxaemia in horses.…”

Section: Discussionmentioning

confidence: 99%

“…L2880, Sigma-Aldrich, Germany) were used at a dose of 0.5 µg/kg as a source of endotoxin, inducing endotoxaemia in sheep (36). The endotoxin was diluted in sterile phosphate buffered saline (PBS, pH 7.4, Sigma-Aldrich, Germany) and then divided into 15 equal doses each containing 0.5 µg/kg of the endotoxin, and stored at −80°C until LPS administration (5,6). Polymyxin B (6,000 and 12,000 U/kg) was intravenously administered to the sheep as a bolus according to an established experimental design (32).…”

Section: Methodsmentioning

confidence: 99%

Effects of polymyxin B on clinical signs, serum TNF-α, haptoglobin and plasma lactate concentrations in experimental endotoxaemia in sheep

Hajimohammadi

Badiei

Kheibari

et al. 2018

Journal of Veterinary Research

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IntroductionThe experiment evaluated the effects of intravenous administration of polymyxin B on experimental endotoxaemia in sheep.Material and MethodsTwenty clinically healthy fat-tailed sheep were randomly divided into: a group treated with 6,000 U/kg of polymyxin B, a group at 12,000 U/kg, and positive and negative controls. Endotoxaemia was induced by intravenous administration of lipopolysaccharide (LPS) from E. coli serotype O55:B5 at 0.5 μg/kg. polymyxin was infused intravenously along with 2.5 L of isotonic intravenous fluids at 20 mL/kg/h. The positive control group received LPS and 2.5 L of isotonic fluids, the negatives receiving just 2.5 L of isotonic fluids. Clinical signs were evaluated before and at 1.5, 3, 4.5, 6, 24, and 48 h after LPS administration. Blood was also sampled at the denoted hours and serum haptoglobin, tumour necrosis factor-α (TNF-α), and plasma lactate concentrations were assayed.ResultsThe serum concentration of TNF-α in the positive control group increased significantly up to 48 h after LPS administration. The concentration of TNF-α was significantly different from those of the polymyxin B and positive control groups from 3 to 48 h; also, the concentrations of haptoglobin at different times in the polymyxin groups were lower than those of the positive control group and were significant at hours 3 to 48 (P < 0.05). Following the LPS administration, haptoglobin and TNF-α concentrations changed without significant difference between the two polymyxin B groups.ConclusionPolymyxin B (6,000 U/kg) restrained blood lactate concentrations. Furthermore, it significantly improved the clinical signs in endotoxaemic animals, including rectal temperature and heart and respiratory rates. Polymyxin B may be an antiendotoxic in fat-tailed sheep.

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“…The degree of dehydration was determined by evaluating the change in body weight and according to the table of estimating the degree of dehydration from the physical examination findings reported by Walz and Taylor (2012). Sheep showing at least two findings of SIRS criteria (body temperature, heart rate, respiratory rate, total leukocyte count) were evaluated as SIRS positive (Badial et al, 2011;Chalmeh et al, 2013;Constable et al, 2016).…”

Section: Clinical Examinationsmentioning

confidence: 99%

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2021

J Hellenic Vet Med Soc

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Modulation of inflammatory responses following insulin therapy in experimentally bolus intravenous Escherichia coli lipopolysaccharide serotype O55:B5 induced endotoxemia in Iranian fat-tailed sheep

Cited by 13 publications

References 25 publications

The Effect of Leptin on the Blood Hormonal Profile (Cortisol, Insulin, Thyroid Hormones) of the Ewe in Acute Inflammation in Two Different Photoperiodical Conditions

The Effect of Leptin on the Blood Hormonal Profile (Cortisol, Insulin, Thyroid Hormones) of the Ewe in Acute Inflammation in Two Different Photoperiodical Conditions

Effects of polymyxin B on clinical signs, serum TNF-α, haptoglobin and plasma lactate concentrations in experimental endotoxaemia in sheep

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