<p>The Regenerated of Siam Tangerin through Somatic<br />Embryogenesis. Ali Husni, Agus Purwito, Ika Mariska,<br />and Sudarsono. Somatic embryogenesis occurs in most<br />plants that are cultured on a suitable medium in vitro.<br />Somatic embryo may arise from single cells and the embryogenic<br />cells are widely applicable in plant propagation,<br />genetic manipulation and transgenic technologies. The<br />present study was carried out to develop an effective<br />somatic embryogenesis technique to regenerate Siam<br />tangerine plants. Materials used in this study were nucellar<br />tissues of young fruits (30-90 days post anthesis). Induction<br />of embryogenic calli was done by culturing the tissues on<br />three different basal media (MS, MW and MT). Embryo<br />maturation was done on the MW medium + ABA (0; 0.1; 0.3;<br />dan 0.5 mg/l), while germination to plantlet development<br />was done on WS medium + GA3 (0, 0.1, 0.3, and 0.5 mg/l).<br />The results showed that among the three media, MW was<br />the best medium for callus induction from nucellar tissues<br />with a success rates 98% for Simadu and 100% for<br />Pontianak. The best maturation of embryo somatic was<br />done on MW medium + ABA 0.5 mg/l with success rates<br />99%, while the best medium for germination and development<br />into planlets was MW medium + 0.5 mg/l GA3 with a<br />success rate 58%.</p>
AbstrakPenelitian ini bertujuan untuk mengetahui ukuran-ukuran tubuh sapi Krui jantan dan betina di Kabupaten Pesisir Barat provinsi Lampung. Penelitian ini dilakukan pada November-Desember 2016. Sampel yang diamati sebanyak 55 ekor sapi jantan dan 71 ekor sapi betina. Metode survey digunakan dalam penelitian ini, dan sampel tersebut ditentukan dengan menggunakan purposive sampling. Data dianalisis dengan uji t. Peubah yang diamati meliputi bobot badan, lingkar dada, panjang badan, dan tinggi pundak. Hasil penelitian menunjukan bahwa rata-rata bobot badan sapi Krui jantan dan betina poel 0 (125,4±117 kg), poel 1 (148,6±136,2 kg), poel 2 (189,6±176,1 kg), poel 3 (234,5±208,4 kg), lingkar dada poel 0 (118±110 cm), poel 1 (132,9±128,3 cm), poel 2 (134,5±129,5 cm), poel 3 (137±130,3 cm), panjang badan poel 0 (103±97,2 cm), poel 1 (110,3±104,1 cm), poel 2 (124,6±109,8 cm) poel 3 (133,6±127,4 cm), sedangkan rata-rata tinggi pundak poel 0 (91,3±86,7 cm), poel 1 (102,5±97,8 cm), poel 2 (105,1±101,1 cm), poel 3 (113±104,7 cm). Hasil penelitian ini menunjukkan bahwa bobot badan, lingkar dada, panjang badan, dan tinggi pundak sapi Krui jantan dan betina pada kelompok umur yang sama menunjukkan perbedaan yang nyata (P<0,05). AbstractThis research was aimed to determine the measurement of male and famale Krui cattles in west Pesisir district Lampung Province. This research was conducted in November up to December 2016. The samples that observed were about 55 male cattles and 71 female cattles. The survey method was In this research, and those samples were determined by using purposive sampling. Data Were analyzed by t test. The observed variables include body weight, chest circumference, body length, and shoulder height. The results showed that the average body weight of male and female Krui permanent teeth of 0 the average shoulder level is permanent teeth of 0 pair (91.3 ± 86.7 cm), permanent teeth of 1 pairs (102,5 ± 97.8 cm), permanent teeth of 2 pairs (105.1 ± 101.1 cm), permanent teeth of 3 pairs (113 ± 104.7 cm). The results of this research show that body weight, chest circumference, body length, high shoulders of male and famale Krui cattles in the same age group showed significant difference (P<0,05).
To increase the genetic diversity of sugarcane can be done through induced mutation using gamma ray irradiation. This research was carried out to determine the response and radiosensitivity of calli sugarcane variety (Kidang Kencana) to gamma ray irradiation, and knowing the diversities of phenotypic mutant of sugarcane. The research was conducted at BATAN and ICECRD, from August 2012 until March 2013. This research was arranged in Completely Randomized Design with 6 doses of gamma ray irradiation (0, 10, 20, 30, 40 and 50 Gy). Each treatment consisted of 10 replications. Each replication consists of 5 clumps of calli. The observed variables were calli fresh weight, percentage of regenerated calli, number of shoots, shoot height, leaves number, roots number and plantlets number, calli and mutant phenotype. The results showed that the ability of calli to regenerate and shoot growth decreased with increasing doses of gamma ray irradiation. Radiosensitivity (LD20-LD50) of sugarcane calli Kidang Kencana variety to gamma irradiation were in the range of 10 and 30 Gy doses. Gamma irradiation 10 and 20 Gy doses caused the variability mutant phenotype were very high. It means that gamma irradiation can be used to increase the genetic variability of sugarcane.
Polyploidy induction could increase shallot bulb-size to raise consumer preference and local shallot productivity. The research aimed to obtain an effective method of polyploidy induction on callus of onion (Allium cepa) var. Bima Brebes. The experiment was consisted of two experimental steps, which were callus induction of onion and polyploid induction of the callus. A 1×1 cm callus was treated by two drops of oryzalin with concentrations 0, 25, 50, 75, 100, and 120 μM. The ploidy level was identified based on morphological trait, stomatal analysis and DNA content using a flow cytometry. The results showed callus diameter, number of green spots, and number of shoots were decreased with increasing oryzalin concentration. The planlet leaves regenerated from oryzalin treated callus were darker than that of control. The flow cytometry analysis showed that planlets with 75 μM oryzalin was tetraploid, had longer and wider stomata than that of the control.
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