Extraction and Identification of Secondary Metabolites from AL6 Isolates and Its Potential as Antibacterial against Escherichia coliABSTRACTSecondary metabolites in the form of antibiotics can be produced by rhizospheric bacteria. AL6 bacterial isolate, which is one of the bacterial isolates from the rhosphere of Saccarum officinarum L., is known to produce antibiotic compounds. This study aims to determine the activity of antibiotics from AL6 ethyl acetate extracts produced by AL6 bacterial isolates, to analyze the minimum inhibitory concentration (MIC) and the similarity of the active substances using GCMS. The ethyl acetate extract obtained was tested for MIC at 1.25%, 2.5%, 5.0%, 10.0%, 20%, and 40% concentrations. Detection of potential antibiotic spots was carried out using bioautographic thin layer chromatography (TLC). Compounds responsible for antibiotic activity were analyzed using GCMS. Minimum inhibitory levels obtained reached 2.5%. The active spots responsible for antibiotic activity against Escherichia coli at Rf 0.94. Components detected using GCMS and suspected to be antibiotics include chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.Keywords: AL6 bacterial isolate; antibiotic; Escherichia coli; GCMS; MICABSTRAKMetabolit sekunder berupa antibiotik dapat diproduksi oleh bakteri rizosfer. Isolat bakteri AL6, salah satu isolat bakteri dari rizosfer Saccarum officinarum L., diketahui dapat menghasilkan senyawa antibiotik. Penelitian ini bertujuan mengetahui aktivitas antibiotik dari ekstrak etil asetat antibiotik AL6 yang dihasilkan isolat bakteri AL6, menganalisis kadar hambat minimum (KHM), serta kemiripan zat aktif menggunakan GCMS. Ekstrak etil asetat yang diperoleh diuji KHM-nya pada konsentrasi 1,25%, 2,5%, 5,0%, 10,0%, 20%, dan 40%. Deteksi bercak yang berpotensi sebagai antibiotik dilakukan menggunakan kromatografi lapis tipis (KLT) bioautografi. Senyawa yang berperan dalam aktivitas antibiotik dianalisis menggunakan GCMS. Kadar hambat minimal yang diperoleh mencapai 2,5%. Hasil uji KLT bioautografi memperlihatkan bercak aktif sebagai antibiotik terhadap Escherichia coli pada Rf 0,94. Komponen senyawa yang terdeteksi menggunakan GCMS dan diduga sebagai antibiotik antara lain chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.
A bacterium is one of microorganisms that can produce secondary metabolite, such as antibiotic. KP13 isolate is a bacterium isolated from Melaleucaleuca dendron L rhizosphere. The aim of this study is to know the eff ect of the most active fraction antibacterium of KP13 isolate toward Escherichia coli bacterium and the ability to caused cell leakage. Extraction of antibiotic was conducted by ethyl acetate. Extract is fractioned with a n-heksan-etilacetate solvent gradient another uses a solvent methanol. Each fraction is done by KLT and grouped based on RF value. A fraction group is compare by inhibition eff ectiveness. Then, the Minimum Inhibitory Concentration (MIC) from the most active group is tested. Cell leakage analysis was performed using UV spectrophotometry to detect the release of nucleic acid and protein. Atomic Absorption Spectrophotometry (AAS) was used to detect ion release of K+ and Ca2+ from. cell of bacteria. The results showed that the most active fraction against Escherichia coli was the fraction number 1 with MIC value of 5% and inhibition zone of 7.33±0.58 mm.
Sampah plastik merupakan salah satu faktor dari sekian banyak faktor penyumbang kerusakan ekosistem. Sedangkan, bahan plastik banyak digunakan dalam berbagai macam kebutuhan hidup manusia sehingga tiap tahun sampah plastik selalu menjadi permasalahan yang sulit diatasi. Tujuan kegiatan ini untuk mengatasi sampah plastik dengan cara simpel dan efektif, yaitu ecobrick. Kegunaan dari ecobrick bukan untuk menghancurkan sampah plastik, tetapi untuk mendaur ulang sampah tersebut dan mengolahnya menjadi sesuatu yang memiliki nilai guna dan nilai jual. Pembuatan ecobrick juga tidak membutuhkan keterampilan khusus dan hanya membutuhkan biaya yang sedikit, karena memanfaatkan sampah plastik rumah tangga sebagai bahan utama. Diharapkan dengan adanya pelatihan dan pendampingan pembuatan ecobrick, masyarakat Dusun Baron dapat mengolah sampah plastik yang selama ini tidak terolah menjadi suatu produk bernilai guna dan nilai jual tinggi.
Traditional medicine is a mixture of various types of plant parts in an area, so that local wisdom related to traditional medicine needs to be studied, preserved, and developed. This requires supporting data, namely in the form of written documentation and documented images with the aim of knowing various types of medicinal plants, types of diseases, how to mix, and how to treat them. This type of research is a descriptive qualitative research with snowball sampling technique. The inclusion criteria in this study, among others: the residents of the Secang District who have sufficient knowledge and experience in traditional medicine, are willing to be informants in the study, people who are over 30 years old. The results showed that the community in 5 villages, Secang District, Magelang Regency used 107 species of medicinal plants from 54 families. The plant parts used are leaves, stems, roots, flowers, seeds, rhizomes, fruits, tubers, all plants, bark, wood, and fruit skins. The results of the above calculations obtained sample results for 5 villages, which amounted to 387 respondents with a 95% confidence level. ICF analysis is used to determine the level of homogeneity between the information provided by the respondents. In this study, there were a total of 80 types of disease, based on the results of the ICF calculation, the value that was close to 1 was 45 efficacy, while the ICF value that was close to 0 was 35 efficacy
Eksplorasi mikroorganisme Actinomycetes dapat menghasilkan senyawa antibiotik. Salah satu bakteri yang dapat menghasilkan senyawa antibiotik adalah isolat bakteri KP13. Tujuan penelitian ini adalah untuk mengetahui karakter fraksi terakitf senyawa antibiotik isolat KP13 menggunakan metode densitometri dan reaksi semprot/ pewarnaan hasil elusi kromatografi lapis tipis. Panjang gelombang yang digunakan untuk densitometri 210 nm serta pereaksi semprot yang digunakan meliputi ninhidrin, Dragendorff, 2,4-dinitrofenil hidrazin (2,4-DNPH), dan anisaldehid asam sulfat. Hasil penelitian menggunakan densitometri pada panjang gelombang 210 nm menunjukkan kromatogram puncak tertinggi pada Rf 0,78. Senyawa yang diduga bertanggung jawab pada nilai Rf 0,78 mempunyai karakteristik senyawa Terpenoid, alkaloid, dan karbonil.
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