EKSTRAKSI DAN IDENTIFIKASI METABOLIT SEKUNDER DARI ISOLAT AL6 SERTA POTENSINYA SEBAGAI ANTIBAKTERI TERHADAP <em>Escherichia coli</em>

Abstract

Extraction and Identification of Secondary Metabolites from AL6 Isolates and Its Potential as Antibacterial against Escherichia coliABSTRACTSecondary metabolites in the form of antibiotics can be produced by rhizospheric bacteria. AL6 bacterial isolate, which is one of the bacterial isolates from the rhosphere of Saccarum officinarum L., is known to produce antibiotic compounds. This study aims to determine the activity of antibiotics from AL6 ethyl acetate extracts produced by AL6 bacterial isolates, to analyze the minimum inhibitory concentration (MIC) and the similarity of the active substances using GCMS. The ethyl acetate extract obtained was tested for MIC at 1.25%, 2.5%, 5.0%, 10.0%, 20%, and 40% concentrations. Detection of potential antibiotic spots was carried out using bioautographic thin layer chromatography (TLC). Compounds responsible for antibiotic activity were analyzed using GCMS. Minimum inhibitory levels obtained reached 2.5%. The active spots responsible for antibiotic activity against Escherichia coli at Rf 0.94. Components detected using GCMS and suspected to be antibiotics include chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.Keywords: AL6 bacterial isolate; antibiotic; Escherichia coli; GCMS; MICABSTRAKMetabolit sekunder berupa antibiotik dapat diproduksi oleh bakteri rizosfer. Isolat bakteri AL6, salah satu isolat bakteri dari rizosfer Saccarum officinarum L., diketahui dapat menghasilkan senyawa antibiotik. Penelitian ini bertujuan mengetahui aktivitas antibiotik dari ekstrak etil asetat antibiotik AL6 yang dihasilkan isolat bakteri AL6, menganalisis kadar hambat minimum (KHM), serta kemiripan zat aktif menggunakan GCMS. Ekstrak etil asetat yang diperoleh diuji KHM-nya pada konsentrasi 1,25%, 2,5%, 5,0%, 10,0%, 20%, dan 40%. Deteksi bercak yang berpotensi sebagai antibiotik dilakukan menggunakan kromatografi lapis tipis (KLT) bioautografi. Senyawa yang berperan dalam aktivitas antibiotik dianalisis menggunakan GCMS. Kadar hambat minimal yang diperoleh mencapai 2,5%. Hasil uji KLT bioautografi memperlihatkan bercak aktif sebagai antibiotik terhadap Escherichia coli pada Rf 0,94. Komponen senyawa yang terdeteksi menggunakan GCMS dan diduga sebagai antibiotik antara lain chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.

SINERGISTIC COMBINATION OF ALOE VERA AND KERSEN LEAF EXTRACTS FOR INHIBITION OF THE GROWTH OF Staphylococcus aureus 25923

“…One loop of bacteria suspended into a sterile BHI DS was scraped then incubated at 37 °C for 2 hours (Syarifuddin, Kamal, et al, 2019). Then, it was diluted to the same turbidity as the 0.5 standard of Mc Farland to make the total bacterial density equivalent to about 1 to 2x10 8 CFU/mL (Clinical And Laboratory Standards Institute, 2015).…”

The Optimization of Antibacterial Production Time of Actinomycetes (J4 Isolate) Against Staphylococcus Aureus and Escherichia Coli

“…Bacterial stock of 100 µL was added to 1 mL of BHI, incubated for 18-24 hours, then 100 µL was taken into 1 mL of BHI, incubated for 3-5 hours in an incubator. Taken 100 µL of bacteria diluted with NaCl 0.9% until the turbidity is equal to the Mc Farland standard of 108 CFU/ mLn [13].…”

Potensi antibiotik fraksi etil asetat ekstrak etanol bunga pepaya jantan (Carica papaya L)

“…Teradapat 3 senyawa yang berpotensi sebagai antibiotik antara lain E-Citral, Linalool, 2-Methoxy-4-vinylphenol, namun penggunaan GC-MS dengan similarity index lebih dari atau mendekati 90% dengan database antara lain Linalool dan 2-Methoxy-4-vinylphenol. Konstituen utama atau dalam kombinasi dengan konstituen minor bertanggung jawab atas aktivitas antibakteri (Syarifuddin et al, 2019).…”