The Optimization of Antibacterial Production Time of Actinomycetes (J4 Isolate) Against Staphylococcus Aureus and Escherichia Coli

Abstract

There are many cases of antibiotic resistance that cause death in patients infected with bacteria. The increase in cases necessitated the discovery of new antibiotics as an alternative to antibiotics that were already resistant. Bacteria that produce antibiotic compounds are located in the rhizosphere of a plant. One of them is the rhizosphere of the red ginger plant (Zingiber officinale Roscoe var. Rubrum). The bacterial isolate that produces new antibiotic compounds in the rhizosphere of red ginger is Isolate J4. Isolate J4 can produce secondary metabolites in the form of antibiotic compounds after inoculation on Starch Nitrate Broth (SNB) medium and incubated in a few days to obtain optimal antibiotic compounds based on their activity. The purpose of this study was to determine the results of the antibiotic activity of secondary metabolites of isolate J4 in producing antibiotics against Staphylococcus aureus and Escherichia coli bacteria. Antibiotic activity profile was observed by looking at the relationship between the inhibition zone and the incubation time of these isolates. The results showed that isolate J4 obtained optimal antibiotic that inhibited the growth of Staphylococcus aureus bacteria after incubation for ten days with an average inhibition zone diameter of 14.2 mm, whereas for Escherichia coli bacteria after incubation for five days which is 12.3 mm in diameter.

Optimization of Antibacterial Metabolite Production and Antioxidant Activity of Endophytic Fungi Isolate from Kelakai (Stenochlaena palustris)

“…The initial stage carried out was optimizing the production time of secondary metabolites. Optimization of production time aims to determine the right time to produce secondary metabolites optimally (Syarifuddin et al, 2021). Optimization was carried out by graphing the relationship between fermentation time and the diameter of the inhibition zone.…”