Isolated zygotic embryos and female gametophytes containing zygotic embryos were cultured on MSG and DCR basal media, supplemented with three different carbon sources added individually to the medium at four levels each. The media also contained various levels of 2,4-dichlorophenoxy acetic acid (2,4-D) and N6-benzyladenine (BA). Embryogenic tissue extruded from female gametophytes during 4 weeks in culture on media containing either glucose or maltose or sucrose. Embryogenic tissue initiation was most frequently from explants collected on July 14, 1992, when the zygotic embryos within the female gametophytes were precotyledonary. A total of 33 embryogenic cultures were initiated from 944 explants cultured. One of 192 explants cultured on basal media with no growth regulators produced embryogenic tissue. The embryogenic tissue showed numerous somatic embryos at stages 1 and 2 of development, corresponding to their zygotic embryo counterparts.
Application. Mature Lark can be serially, clonally micropropagated in vitro. Potentially, the most promising mature phenotypes may be selected and rapidly cloned for outplanting.Abstract.Adventitious buds were developed in vitro from stems of elongating short shoots of mature European larch (Lark decidua Mill.). Tissue culture propagule populations thus generated, were similarly remultiplied in vitro. Elongation and rooting were routine. No vitreous or otherwise abnormal shoots were noted.
Hypersensitive resistance to axenically cultured Cronartium ribicola was displayed by subcultured callus of Pinus lambertiana. Cellular resistance to a destructive rust disease can now be studied at the macromolecular level through use of cloned cells of both host and pathogen in a system amenable to emerging recombinant DNA technology.
Young seedlings and calli of European iarch {Larix decidua Mill.) but not jack pine (Pinus banksiana Lamb.) were rapidly infected with Agrobacterium rhizogenes (Riker et al.) Conn. Calli were extensively colonized and necrotic within 2 weeks. Seedlings swelled at the wound site, and developed multiple buds, blue-colored needles with dense aggregations of stomates, or adventitious roots. Inoculation of juvenile conifer tissues with selected strains of plasmid donors may offer opportunity to clone engineered superior tree genotypes through in vitro micropropagation.
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