Induction of roots on jujube softwood cuttings using<i>Agrobacterium rhizogenes</i>

Hatta, Muhammad; Beyl, Caula A.; Garton, Stephen; Diner, Alex M.

doi:10.1080/14620316.1996.11515470

Cited by 21 publications

(6 citation statements)

References 12 publications

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“…Average total root lengths for A4 and H 2 O cuttings were not statistically significant in both repetitions of this experiment (Figures 1d and 2d), suggesting no improved rooting abilities of cannabis cuttings inoculated with R. rhizogenes. Comparable results were reported by Hatta [35] on softwood cuttings of two jujube (Ziziphus jujuba) cultivars ("Contorta" and "Li"). Ten weeks after inoculation with strains A4 and TR105 of R. rhizogenes, neither strain had an effect on the length of the longest root compared to water controls, although the longest roots of "Li" cuttings were significantly longer than those of "Concorta" cuttings (p ≤ 0.05), suggesting different innate rooting abilities between cultivars [35].…”

Section: Discussionsupporting

confidence: 50%

“…Comparable results were reported by Hatta [35] on softwood cuttings of two jujube (Ziziphus jujuba) cultivars ("Contorta" and "Li"). Ten weeks after inoculation with strains A4 and TR105 of R. rhizogenes, neither strain had an effect on the length of the longest root compared to water controls, although the longest roots of "Li" cuttings were significantly longer than those of "Concorta" cuttings (p ≤ 0.05), suggesting different innate rooting abilities between cultivars [35]. A similar cultivar difference was found when measuring the number of roots per cutting, which also revealed significantly more roots (p ≤ 0.05) in cuttings inoculated with the TR105 strains, whereas the A4 strain yielded no more roots on average than water controls [35].…”

Section: Discussionsupporting

confidence: 50%

“…Ten weeks after inoculation with strains A4 and TR105 of R. rhizogenes, neither strain had an effect on the length of the longest root compared to water controls, although the longest roots of "Li" cuttings were significantly longer than those of "Concorta" cuttings (p ≤ 0.05), suggesting different innate rooting abilities between cultivars [35]. A similar cultivar difference was found when measuring the number of roots per cutting, which also revealed significantly more roots (p ≤ 0.05) in cuttings inoculated with the TR105 strains, whereas the A4 strain yielded no more roots on average than water controls [35]. Increased total root lengths (p ≤ 0.01) and root dry weights (p ≤ 0.05) have been shown in mint (Mentha piperita) cuttings upon inoculation with R. rhizogenes (strain A16), although root dry weights were increased more by inoculation with Bacillus megatorium (strain M3) than R. rhizogenes.…”

Section: Discussionmentioning

confidence: 83%

“…Bands for rolB were found in 21 of 34 cuttings (62%) (Figure 4). Plasmid remnants indicated by virD bands were seen in 32% of the cuttings (Figure 4; samples 2, 3, 7, 9, 10,11,14,17,23,34,35), all of which were of the A4 treatment group. The rolB bands were not present in the nutrient solutions (Figure 4; samples 200, 201, 202) as well as in root tissue from an IBA-and an H2O cutting; similarly, no bands for virD were observed (Figure 4; samples 46, 81).…”

Section: Detection Of Rolb Vird and Ef1α Gene Fragments In Root Tissu...mentioning

confidence: 99%

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Alternative Rooting Methods for Medicinal Cannabis Cultivation in Denmark—Preliminary Results

Favero,

Salomonsen,

Lütken

2023

Plants

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Cannabis sativa L. is typically propagated through micropropagation or vegetative cuttings, but the use of root-inducing hormones, such as indole-3-butyric acid (IBA), is not allowed for growing medicinal cannabis in Denmark. This study examined alternative rooting treatments, including Rhizobium rhizogenes inoculation, water-only as well as IBA treatments, in eight cannabis cultivars. PCR on root tissue suggested that 19% of R. rhizogenes-inoculated cuttings were transformed. These were derived from “Herijuana”, “Wild Thailand”, “Motherlode Kush”, and “Bruce Banner”, indicating a variation in cultivar susceptibility toward R. rhizogenes. A 100% rooting success was achieved regardless of cultivar and treatment, suggesting that alternative rooting agents are not required for efficient vegetative propagation. However, rooted cuttings differed in shoot morphology with improved shoot growth in cuttings treated with R. rhizogenes (195 ± 7 mm) or water (185 ± 7 mm) while inhibited shoot growth under IBA treatment (123 ± 6 mm). This could have advantageous economic implications should cuttings not treated with hormone reach maturity faster than those exposed to the hormone, thereby contributing to completing a full growing cycle more effectively. IBA exposure increased root length, root dry weight, and root/shoot dry weight ratio compared to cuttings treated with R. rhizogenes or water but simultaneously inhibited shoot growth compared to these.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 83%

Section: Detection Of Rolb Vird and Ef1α Gene Fragments In Root Tissu...mentioning

confidence: 99%

See 2 more Smart Citations

Alternative Rooting Methods for Medicinal Cannabis Cultivation in Denmark—Preliminary Results

Favero,

Salomonsen,

Lütken

2023

Plants

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“…Zydlik and Zydlik (2008) reported that the EM preparation intensified the growth of M.9 rootstock. Recent studies proved that some bacterial genera induced root formation in stem cuttings (Bassil et al, 1991;Ercisli et al, 2004;Erturk et al, 2010;Hatta et al, 1996;Jacob et al, 1991;Rinallo et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

The influence of treatments applied to common ninebark (Physocarpus opulifolius (L.) Maxim.) ‘Diabolo’ cuttings on their rooting and growth

Świerczyński¹,

Stachowiak²,

Golcz-Polaszewska³

2017

Nauka Przyr. Technol.

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Agrobacterium rhizogenes-Mediated Transformation and Its Biotechnological Applications in Crops

2013

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The history of Agrobacterium-related plant biotechnology goes back for more than three decades with the discovery of molecular mechanisms of crown gall disease in plants. After 1980s, gene technologies began developing rapidly and today, related with the improved gene transfer methods, plant biotechnology has become one of the most important branches in science. Till now, the most important genes related with agricultural affairs have been utilized for cloning of plants with the deployment of different techniques used in genetic engineering. Especially, Agrobacterium tumefaciens was used extensively for transferring desired genetic materials to plants rapidly and effectively by the researchers to create transgenic plants. Recognition of the biology of Agrobacterium species and newly developed applications of their T-DNA systems has been a great step in plant biotechnology. This chapter provides the reader with extensive information on A. rhizogenes which is responsible for the development of hairy root disease in a wide range of dicotyledonous plants and its T-DNA system. This knowledge will be useful in improving utilization of crops and the formulation of new and up-graded transgenic based food products.

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Induction of roots on jujube softwood cuttings usingAgrobacterium rhizogenes

Cited by 21 publications

References 12 publications

Alternative Rooting Methods for Medicinal Cannabis Cultivation in Denmark—Preliminary Results

Alternative Rooting Methods for Medicinal Cannabis Cultivation in Denmark—Preliminary Results

The influence of treatments applied to common ninebark (Physocarpus opulifolius (L.) Maxim.) ‘Diabolo’ cuttings on their rooting and growth

Agrobacterium rhizogenes-Mediated Transformation and Its Biotechnological Applications in Crops

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