Food contamination has become critical issue and is being worse due to the insensitive detection devices. One of the dangerous food contaminations is by Escherichia coli (E.coli) O157:H7, one of the harmful bacterial pathogens which is distributed in soil, marine and estuarine waters, the intestinal tract of animals, or water contaminated with fecal matter. A small amount of E.coli with the dose fewer than 100 organisms in food products or water is enough to cause serious gastrointestinal illness to human. Hence, the ultra-high sensitive, label free biosensors have been designed in this research for the low concentration E.coli detection. Surface acoustic waves (SAW) devices have been initially developed and used for the high-volume low-cost TV component. Due to the ultra-sensitivity to the surface perturbation, SAW based devices have been modified to be sensors. Initially, SAW sensors were developed for gas detections and have been moving towards biological detections recently. Shear horizontal surface acoustic wave (SHSAW), one of the SAW based types is most suitable for the liquid based application as it has the advantage of acoustic energy is not being radiated into liquid. However, the main SHSAW design components are the operating frequency and wave length. These are strictly depended on the inter digital transducers (IDTs) design. Therefore, this paper is presenting the IDTs design concept and pattern development by using computer aid design (CAD) software.
Surface acoustic waves based devices were initially developed for the telecommunication purpose such as signal filters and resonators. The acoustic energy is strongly confined on the surface of the surface acoustic waves (SAW) based devices and consequent their ultra-sensitivity to the surface perturbation. This has made SAW permits the highly sensitive detection of utterly diminutive charges on the surface. Hence, SAW based devices have been modified to be sensors. Food contamination has become critical issue and sensitive detection devices are needed urgently as small amount of harmful bacterial pathogens such as Escherichia coli (E.coli) O157:H7with the dose fewer than 100 organisms in food products or water is enough to cause serious gastrointestinal illness to human. Therefore, ultra-high sensitive, label free biosensors have been designed in this research for the low concentration E.coli detection. After the saturated development in telecommunication filed, SAW sensors were developed for gas detections and have been moving towards biological detections recently. Shear horizontal surface acoustic wave (SHSAW), one of the SAW based types is most suitable for the liquid based application as it has the advantage of acoustic energy is not being radiated into liquid. Therefore, SHSAW device has the potential to provide high-performance sensing platform in this research. There have been a lot of complicated theoretical models for the SAW devices development since 1960 as signal filters and resonators such as from delta function model, equivalent circuit model, to the current SAW models such as coupling-of-modes (COM) model, P-matrix model and finite element analysis (FEA) model. However, SHSAW device in this research is not meant for signal filter or resonators but used for surface sensing purpose, therefore the simplicity method of the modeling is presented in the paper for the E.coli detection sensor development.
Surface acoustic wave can be generated at the free surface of an elastic solid. Interdigital transducers (IDTs) are fabricated on the piezoelectric substrate surface that will act as electrical input and output port. When appropriate AC voltage stimulus is applied to the input transducer, surface acoustic wave will be produced. The output or receiving port will detect the incident surface acoustic wave and convert it back to a suitably filtered electrical once. For this property, surface acoustic based devices were initially developed for the telecommunication purpose such as signal filters and resonators. SAW based devices have been modified to be sensors later on from for gas detections and have been moving towards biological detections recently for its ultra-sensitivity to surface perturbation. The main component of this device is the IDTs. Recently, there are several methods to produce IDTs; Ultra-Violet (UV), deep UV lithography, Electron beam (e-beam) lithography and X-ray lithography. Although, these methods can produce very fine and accurate electrodes in term of submicron size but the costs are extremely expensive. Thus, this paper will discuss the conventional CMOS method which is much more economical to produce the applicable IDTs for the bacterial pathogens sensing purpose. Shear horizontal surface acoustic wave (SHSAW), one of the SAW based types is used in this paper as it is most suitable for the liquid based application as it has the advantage of acoustic energy is not being radiated into liquid.
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