Namnam (Cynometra cauliflora L) and trigona honey are two potential natural ingredients to b e developed in Indonesia. However, scientific evidence of their active compounds and b ioactivity is still rarely found, particularly the combination of these two materials. Therefore , this research aimed to explore the active ingredients of the combination of the two materials, i.e. total phenolic, flavonoids, vitamin C and βcarotene and its b ioactive capabilities such as antioxidant activity and antibacterial activity. The analysis showed the methanol extract of Nam nam leaves (EMDN) and trigona honey either in their sole form or combined form has antioxidant and antib acterial activity. The EMDN was more active to Staphylococcus aureus (23.7±3.3 mm) than to Escherichia coli, while the Trigona honey (MT) more active to Escherichia coli (32.6±4.4 mm) than Staphylococcus aureus (16.6±4.1 mm). Similarly, the combination of EMDN and MT showed higher activity to Escherichia coli (23±1.9 mm) than Staphylococcus aureus (17.6±2.6 mm). Analysis of the antioxidant activity also showed that EMDN provided the highest activity with an IC 50 of 0.0048±0.000 mg/mL), while a comb ined EMDN and MT had an IC 50 of 0.0085±0.000 mg/mL) and MT with an IC50 of 3.736±0.112 mg/mL. Moreover, this analysis also showed that sole samples of MT and EMDN have total phenolic content, flavonoids, and vitamin C that were higher than the comb ination of MT and EMDN. However, the content of β-carotene in the combined form of MT and EMDN was higher. Thus trigona honey, methanol extract of leaves Nam nam (Cynometra cauliflora L) in a single form or in a combination are potential to b e utilized and developed as a source of antioxidants and antibacterial in the form of functional food.

AbstrakSerat pangan telah banyak digunakan sebagai pangan fungsional dan direkomendasikan untuk menurunkan kadar lipid darah untuk mencegah hiperkolesterolemia. Bekatul merupakan bahan pangan yang mengandung serat cukup tinggi. Tujuan penelitian untuk mempelajari pengaruh penambahan bekatul pada parameter lipid darah mencit jantan hiperkolesterolemia. Penelitian dilakukan di kandang hewan Jurusan Pendidikan Biologi Universitas Pendidikan Indonesia dan Laboratorium Fisiologi Fakultas Kedokteran Hewan Institut Pertanian Bogor periode Agustus 2011-Maret 2012. Desain penelitian menggunakan rancangan acak lengkap dengan lima kelompok perlakuan, yaitu kontrol negatif mencit normokolesterolemia yang diberi pakan standar tanpa suplementasi bekatul dan kontrol positif mencit hiperkolesterolemia tanpa suplementasi bekatul, selanjutnya kelompok mencit hiperkolesterolemia diberi suplementasi bekatul 16%, 38%, dan 57%. Parameter yang diukur ialah bobot badan, konsentrasi kolesterol total serum, hati, dan feses, kadar trigliserida, high density lipoprotein (HDL-c), low density lipoprotein (LDL-c), dan glukosa darah. Hasil penelitian menunjukkan bahwa suplementasi bekatul dalam diet menurunkan bobot badan, konsentrasi kolesterol serum total dan hati, trigliserida dan LDL-c, serta menaikkan konsentrasi HDL-c dan kolesterol feses, tanpa mengubah konsentrasi glukosa darah. Suplementasi bekatul sebesar 57% menurunkan bobot badan sebesar 10,31%, kadar total kolesterol 17,28%, trigliserida 28,63%, dan LDL-c 79,35%, serta meningkatkan HDL-c sebesar 24,41%. Suplementasi bekatul menurunkan kolesterol hati 57,76% dan meningkatkan pembuangan kolesterol melalui feses sebesar 39,86%. Simpulan, bekatul sebagai suplemen makanan memperbaiki parameter lipida darah mencit jantan hiperkolesterolemia dengan meningkatkan pembuangan kolesterol melalui feses dan menurunkan bobot badan tanpa mengubah kadar glukosa darah. [MKB. 2013;45(1):1-9] Kata kunci: Bekatul, hiperkolesterolemia, lipid darah, mencit, serat pangan Improvement of Blood Lipid Parameters of Hypercholesterolemic Mice by Supplementation of Rice Bran AbstractDietary fiber is widely used as a functional food and recommended to reduce blood lipid level to prevent hypercholesterolemia. An experiment was conducted on the effects of rice bran that has high dietary fiber content on blood lipid parameters of hypercholesterolemic male mice, which was conducted at the Biology Education Department animal cages, Indonesia University of Education and the Veterinary Medicine Faculty Laboratory, Bogor Agricultural Institute from August 2011 to March 2012. The mice were randomly assigned into groups with 5 different treatments: negative control group i.e. normocholesterol mice with a standard diet without rice bran; positive control group i.e. hypercholesterolemic mice with a standard diet without rice bran; and groups with hypercholesterolemic mice with 16%, 38%, and 75% rice bran supplements. The parameters were body weight as well as blood serum, liver and feces cholesterol, triglyceride, high den...

ABSTRAKKecamatan Pangalengan berada pada ketinggian 10001400 mdpl dengan suhu per tahun berkisar antara 1228°C dengan kelembapan per tahun 6070%. Kondisi lingkungan Pangalengan ini dapat memengaruhi kondisi fisiologis dan kesehatan sapi perah, khususnya pada saat kering kandang. Informasi tentang nilai fisiologis sapi pada saat kering kandang sampai saat ini belum tersedia. Penelitian menggunakan 46 ekor sapi perah kering kandang dan pengukuran parameter fisiologis dilakukan pada pagi, siang, dan sore hari. Data dianalisis menggunakan sidik ragam dan dilanjutkan dengan uji beda nyata terkecil. Kisaran denyut jantung, respirasi, dan suhu tubuh secara berturutturut adalah sebesar 52,870,2 kali/menit, 18,936,6 kali/menit, dan 37,638,6°C. Kisaran hemoglobin, hematokrit, eritrosit, dan leukosit adalah 9,311,3 g/dl; 30,436,6%; 6,58,7 juta/µl, dan 7,412,8 ribu/µl. Kisaran diferensial leukosit meliputi limfosit, neutrofil, monosit, eosinofil, dan basophil secara berturut-turut adalah sebesar 29,6055,60%; 28,8056,20%; 0,304,30%; 5,5019,7%; dan 0,000,00%. Rasio neutrofil/limfosit didapatkan berkisar antara 0,451,91. Studi ini menyimpulkan bahwa sapi perah kering kandang yang dipelihara pada kondisi iklim Pangalengan yang sejuk menunjukkan nilai fisiologis yang berada dalam kisaran normal.Kata kunci: kering kandang, nilai fisiologis, pangalengan, sapi perah ABSTRACT Pangalengan is at an altitude of 10001400 m above sea level with annual temperatures range of 1228°C and annual humidity of 6070%. Pangalengan's environmental conditions can affect the physiological conditions and health of dairy cattle, especially during the dry period. The information about the physiological value of dairy cows when they are in a dry period is not yet available. This study used 46 dairy cows that were on a dry period, and measurements of physiological parameters were carried out in the morning, afternoon, and evening. Heart rate, respiration rate, and body temperature ranges were 52,870,2 times/min, 18.9−36.6 times/min, and 37,638,6°C, respectively. The ranges of hemoglobin, hematocrit, erythrocytes, and leukocytes were 9.311.3 g/dl; 30.436.6%; 6.58.70 million/µl; and 7.412.8 thousand/µl, respectively. The differential ranges of leukocytes including lymphocytes, neutrophils, monocytes, eosinophil, and basophils were at 29.6055.60%; 28.8056.20%; 0.304.30%; 5.5019.7%; and 0.000.00%, respectively. The neutrophil/lymphocyte ratio was obtained in the range of 0.451.91. This study concluded that dairy cows during dry period maintained in mild Pangalengan climatic conditions showed physiological values that were within the normal range.

Penelitian ini bertujuan menguji pengaruh tombong kelapa yang dicampurkan ke dalam pakan terhadap parameter kesehatan tikus putih betina dara melalui nilai hematologi lengkap. Parameter hematologi yang dianalisa meliputi hemoglobin, eritrosit, leukosit, hematokrit, dan diff erensial leukosit. Penelitian ini menggunakan 4 kelompok perlakuan dosis tombong kelapa 0 mg, 50 mg, 100 mg, dan 200 mg/ekor/hari, masing-masing terdiri dari 6 ulangan. Serbuk kasar tombong kelapa dicampurkan ke dalam pakan komersil dan dibentuk kembali menjadi pakan pelet. Dosis campuran tombong kelapa tersebut diberikan sebanyak satu kali/hari. Sampel darah diambil selama 4 minggu. Hasil penelitian menunjukkan, kadar hemoglobin tidak meningkat secara nyata (P>0,05). Jumlah Hb normal, yaitu 11.93-15.19 g%. Eritrosit dan leukosit, menunjukkan peningkatan dan penurunan secara nyata (P<0,05). Rata-rata jumlah eritrosit 6.03-8.96 juta/mm3, leukosit 7.18-15.77 ribu/mm3 terindikasi normal. Leukosit di minggu keempat pada dosis 50 mg, 100 mg, dan 200 mg/ekor/hari menurun dari batas normal, yaitu 6-10 ribu/mm3. Hematokrit tidak meningkat secara nyata (P>0,05). Diff erensial leukosit, yaitu limfosit minggu pertama, dan monosit minggu ketiga meningkat signifi kan (P>0,05). Netrofi l di minggu pertama terjadi penurunan secara signifi kan (P<0,05) dibandingkan kelompok kontrol. Eosinofi l menunjukkan perubahan secara signifi kan di minggu ketiga (P<0,05). Rata-rata nilai eosinofi l berada dalam jumlah normal, yaitu 1-4%. Basofi l tidak ditemukan pada pemberian diet pakan tombong kelapa. Kesimpulan bahwa, tombong kelapa tidak berpengaruh pada homoestasis darah. Dosis yang terbaik yaitu 50 mg, dan 100 mg/ekor/hari.

ABSTRAKPangalengan terletak di wilayah pegunungan dengan ketinggian 1.0001.420 dpl, yang memiliki rataan temperatur sekitar 17,80 ± 1,46 C dan kelembapan 63,99 ± 2,74%. Kondisi ini sangat mungkin memengaruhi nilai fisiologis sapi perah, terutama selama periode laktasi. Namun demikian, informasi tentang nilai fisiologis sapi laktasi di Pangalengan sampai saat ini belum tersedia. Penelitian ini menggunakan 20 ekor sapi perah, dan nilai fisiologis diukur pada pagi, siang, dan sore hari. Kisaran frekuensi denyut jantung, respirasi, dan temperatur rektal sapi perah laktasi secara berturut-turut adalah 59,8272,02 kali/min, 26,0136,69 kali/min, dan 37,3238,36 C. Pangalengan merupakan salah satu kecamatan di Kabupaten Bandung, yang merupakan sentra peternakan sapi perah di Indonesia. Secara geografis, wilayah Pangalengan berada pada ketinggian 1.0001.420 mdpl memiliki suhu udara 1228 C dan kelembapan relatif 6070% (Qodarudin 1993). Mikroklimat suatu wilayah seperti temperatur udara, kelembapan, tekanan udara, kecepatan angin, dan arah angin memengaruhi parameter fisiologis ternak, terutama pada frekuensi respirasi, denyut jantung, dan suhu rektal (Suprayogi et al. 2013a). Kondisi fisiologis sapi yang ada di wilayah peternakan dapat bergeser dari zona nyaman (termonetral) ke kondisi yang tidak nyaman (stres), sebagai akibat dari berbagai faktor diantaranya pergeseran iklim. Pergeseran iklim ini dapat meme-1 Departemen

<p>Institute for Research and Community Service Bogor Agricultural University (LPPM IPB) has excellent performance in community service activities (PPM). LPPM IPB since 2009 has conducted various community service activities in rural and urban campus area around of Campus. One of the activities is related with sheep and supporting activities. This assessment activity has two (2) purposes: first, to measure the knowledge of farmers in the application of science and technology especially cage and cultivation that has been given, and the second one, to give appreciation for the achievements of farmers in the management of sheep, especially for the category of healthy and productive cage. This activity was carried out by field visits and interviews using a special form of assessment. 17 breeders were determined based on nonprobability purposive sampling method. The highest value obtained Nurpandi farmer from the Village Sukawening got 920.9 points, while the lowest value obtained Sahedi farmer from the Village Purwasari got 308.3 points. Conclusions from the results obtained showed mostly cage sheep (52.94%) have not yet reached the category of healthy and productive cage (final value of less than 800 points).</p><p> </p>

Dugong are endangered herbivorous marine mammals, which one of the causes of extinction is degradation of seagrass as main feed of the dugong to survive. Aim of this study was to measure the quality of seagrass of wild dugong in Lingayan island, Central Sulawesi. The results showed proximat analysis value of Halophyla minor, Halodule uninervis and Cymodocea rotundata as seagrass species respectively showed values crude protein of 6.86%, 7.69% and 8.79%, crude fiber of 10.77%, 18.36% and 24.26 %, crude fat of  0.99%, 1.81 and 1.5%, calcium of 0.79%, 2.12% and 1.89%, phosphorus of  0.34%, 0.34% and 0.26 and gross energy of 163.4 cal / kg, 300.5 cal / kg and 319.5 cal / kg. Cymodocea rotundata is the best feed for dugong because it contains high energy of 319.5 cal/kg and high protein 8.79%,  if compared with Halophyla minor of 1622 cal/kg) and Halodule uninervis of 3014 cal/kg) furthermore Cymodocea rotundata has a fairly high crude fat content of 1.44% which will increase fat levels under the skin (subcutaneous ) dugong as insulators of changing environmental conditions. High concentrations of fiber in Cymodocea rotundata 25.26% can help retain water as long as food passes in the intestine. The levels of nutrients in Cymodocea rotundata which found in Lingayan island deserve highest nutritious feed of dugong.

This research was designed to determine the efficacy of various preparations of katuk (Sauropus androgynus L.) leaves on productivity and carcass quality of broiler chiken. This research used experimental methods of block randomized design. Experimental animals used in this study were 100 male broiler chickens (Ross) with body weight ±40 g. The chickens were divided into five experimental groups, namely: control group (control), katuk leaves powder (TDK), extract of dried katuk (EKK), extract of brewed katuk (EKS) and katuk juice (KP). Each treatment consisted of twenty chickens. The administration of feed treatment every day from the seventh days to 37th days-old chicken (age 1th to 5th) ad libitum. The results showed that the administration of preparation extract and juice (EKK, EKS and KP) leaves katuk able to improve carcass quality and prevent the decline in productivity growth in body weight compare with leaf meal preparation katuk (TDK) real body weight can reduce the growth of broiler chickens is 1645,80 g/individual (EKK), 1450,30 g/individual (EKS), 1472,50 g/individual (KP) and 1408,90 g/individual (TDK). The administration of various preparations of katuk had a positive response, especially in improving carcass quality characterized by the decrease in abdominal fat deposits, level of fat and cholesterol broiler meat. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh berbagai sediaaan katuk (Sauropus androgynus L.) terhadap produktivitas dan kualitas daging ayam pedaging/broiler. Penelitian ini menggunakan rancangan acak kelompok (RAK). Hewan coba yang digunakan dalam penelitian ini sebanyak 100 ekor ayam broiler jantan (strain Ross) bobot badan sekitar 40 g yang dikelompokan ke dalam lima kelompok perlakuan pakan, yaitu: kontrol, tepung daun katuk (TDK), ekstrak katuk kering (EKK), ekstrak katuk seduh (EKS), dan katuk perasan (KP). Setiap perlakuan terdiri atas 20 ekor ayam. Pemberian pakan perlakuan dilakukan setiap hari mulai usia hari ke tujuh sampai ayam berusia 37 hari(minggu ke-1 sampai mingu ke-5) ad libitum. Hasil penelitian menunjukkan bahwa pemberian sediaan ekstrak dan perasan (EKK, EKS, dan KP) daun katuk mampu meningkatkan kualitas karkas dan mencegah penurunan produktivitas pertumbuhan bobot badan dibandingkan dengan sediaan tepung daun katuk (TDK) yang nyata dapat menurunkan pertumbuhan bobot badan ayam broiler yaitu 1645,80 g/ekor (EKK), 1450,30 g/ekor (EKS), 1472,50 g/ekor (KP), dan 1408,90 g/ekor (TDK). Pemberian berbagai sediaan katuk memiliki respons positif terutama dalam peningkatan kualitas karkas ditandai dengan penurunan deposit lemak abdominal, kadar lemak, dan kolesterol daging ayam broiler.