The aim of this study was to evaluate the antidiabetic activity of LH II purified from ethanolic seed extract of Eugenia jambolana in alloxan-induced mild diabetic (MD) and severely diabetic (SD) rabbits. Ethanolic extract upon chromatographic purification yielded partially purified hypoglycemic principle (SIII) which on further purification by sephadex LH 20 yielded pharmacological active compound LH II. Homogeneity of LH II was tested by HPLC. Phytochemical investigation of LH II by various structural spectra showed the presence of saturated fatty acid, Δ5 lipid and presence of sterol. LH II was administered orally at a dose of 10 mg kg−1 body weight to MD and SD. LH II resulted, significant fall in FBG at 90 min (21.2% MD: 28.6% SD), 7th day (35.6% MD) and 15th day (59.6% SD). Glycosylated hemoglobin was significantly decreased (50.5%) in SD after 15 days treatment (Tt). Plasma insulin levels were significantly increased (P < .001). In vitro studies with pancreatic islets showed 3-fold increase in insulin levels as compared to untreated animals. LH II also showed extrapancreatic effect by significantly increasing (P < .001) the activity of key enzymes of glycolysis and significantly decreasing (P < .001) the activity of key enzymes of gluconeogenesis. Liver and muscle glycogen content were increased by 36.6 and 30% for MD, and 52 and 47% for SD, respectively. Thus, the present study demonstrates that LH II possesses potent antidiabetic activity and it is effective in both MD and SD rabbits.
To report the management of an iatrogenic perforation of pulpal floor in the furcation of mandibular first molar, using Mineral Trioxide Aggregate (MTA) and platelet rich fibrin (PRF). Unpredictable endodontic root/pulp chamber floor perforations resulting in unacceptable high rate of clinical failure has now been a lesser threat with the advent of new technologies and biocompatible materials that utilize the applications of basic research along with tissue engineering concept in clinical practice. Present case report illustrates the use of MTA and platelet rich fibrin (PRF) for the repair of the perforation defect and regeneration of the lost periodontium in furcation area. Although, histologic events and reaction of MTA with PRF is not studied so far, however, the autologous and biocompatible nature of the components used for present treatment modalities seems to be beneficial for the long term clinical results obtained in our case.
Hepatoprotective effect of the aqueous leaf extract of Andrographis paniculata was investigated against CCl4 -induced hepatic injury in rats. Significant (P<0.05) increase of serum levels of alanine aminotransferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), total bilirubin (TBL), direct bilirubin (DBL), total cholesterol (CHL), triglycerides (TG), low density lipoprotein (LDL), very low density lipoprotein (VLDL) and malondialdehyde (MDA) in CCl4 intoxicated rats were restored to normal levels when treated with the extract and CCl4. Significant (P<0.05) decrease of serum levels of total protein (TP), albumin (ALB), high density lipoprotein (HDL) and reduced glutathione (GSH) in CCl4 intoxicated rats were restored to normal levels when treated with the extract and CCl4. The LD50 of the leaf extract was greater than 3000 mg/kg. The study demonstrated that A. paniculata possesses significant hepatoprotective effects and may be the source of lead compound in the management of liver diseases.
Diabetes is accompanied by lipid abnormalities, which contribute significantly to cardiovascular morbidity and mortality in diabetic patients. We previously demonstrated the potent antihyperglycemic activity of the active principle (fraction II from Sephadex LH 20 chromatography [LH II]) isolated from ethanolic seed extract of Eugenia jambolana in diabetic rabbits. In the present study, the efficacy of LH II was evaluated for its hypolipidemic activity in alloxan-induced mildly diabetic (MD) and severely diabetic (SD) rabbits. Phytochemical investigation of LH II by various structural spectra showed the presence of saturated fatty acid, Δ(5) lipid, and sterol. Oral administration of LH II (10 mg/kg of body weight) for 21 days resulted in improved glycemic control in both MD and SD rabbits. After treatment with LH II, serum total cholesterol, triglycerides, high-density lipoprotein cholesterol, and the total cholesterol/high-density lipoprotein cholesterol ratio were significantly improved. LH II also resulted in significant (P < .001) improvement in 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and levels of total lipids and glycogen in both MD and SD rabbits. Thus, the present study demonstrates that LH II possesses potent hypolipidemic activity and efficacy in both MD and SD rabbits.
The liver is a primary storage site for a variety of metabolites. It plays a role in wide variety of metabolic, toxic, excretory, circulatory and defence functions inside the body. Damage to the organ may not evidently affect its activity since the liver has considerable functional reserve. Liver disease is a common term for any damage that reduces the functioning of the liver. Chronic liver disease is identified by gradual destruction of liver cells resulting in fibrosis. It is affected by different conditions including viral hepatitis, excessive alcoholism, genetic, autoimmune and NAFLD. The present study was carried out in Govt. Medical College, Jalaun at Department of Biochemistry. The diagnosis of Liver disease was done by ultrasonographic examination of liver. This study comprised a total of 50 patients, 25 of whom were healthy controls and 25 of whom were Liver Disease patients. After overnight fasting 8-12 hours (under aseptic condition) blood sample (8ml) was drawn from antecubital vein of each subject using a plain vial and was analyzed for serum Cu, Zn & liver profile parameters (serum bilirubin, serum SGOT, serum SGPT and serum ALP done by colorimetric method and calmagnite method, diazo method, IFCC method and assessed by kinetic method respectively. The present study’s findings, indicates that the level of serum copper in liver disease patient was 127.38±28.81µg/dl, which is significantly higher than that of 86.54 ± 15.88µg/dl found is healthy controls (p<0.001). Also, serum copper is strongly linked with biochemical parameters of liver enzyme (SGOT & SGPT). Likewise, the mean value of serum zinc in liver disease were 58.08 ± 13.11µg/dl, which is significantly lower than that of69.88± 6.67µg/dl found in healthy subjects. Further, serum zinc has shown a significant weakly linked with serum SGPT.Therefore, during routine assessments of individuals with liver disease, serum trace elements (Cu and Zn) can be highly effective indicators for detecting the severity of liver damage. Thus, based on the findings of our current investigation, zinc and copper supplementation, as well as a reduction in copper intake, may help to enhance patient survival and preventing the development of hepatitis B to liver cirrhosis.Considering findings of the study, it is recommended that serum trace element concentrations be corrected on a regular basis to help with various problems of liver cirrhosis and maybe to slow the progression of liver disease.
Background: Diabetes mellitus (DM) is one of the main chronic health conditions of the 21st century. The diabetes prevalence is rising worldwide due to growth in population, aging, urbanization, and the obesity increase due to physical inactivity. DM is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion and insulin action or both. Prediabetes or impaired glucose in fasting is halfway between normal glucose levels and diabetes diagnostic levels. Aims and objectives: To evaluate the hyperglycemia-induced oxidative stress status in diabetes mellitus type-2. Methods: The prospective case-control study was conducted at the Index medical college, Malwanchal University, Indore, M.P. on 400 patients of Type 2 DM attending OPD who were 30- 70 years of age. Patients with chronic diseases like Chronic Kidney Disease, COPD, cancer, Metabolic disorders like Hypothyroidism, Infectious diseases like TB, HIV, Hepatitis, Pregnant and breast feeding women, etc, were excluded from the study. Results: 64.0% were male and the rest were female studied patients. 29.3% of cases were having a family history of diabetes and 16.3% of cases were parental and statistically insignicant distribution in both groups (P>0.05). The duration of diabetes was 13.94±5.45 years and 89.7% of cases were uncontrolled diabetes, of which 59.7% cases were on the oral glycaemic drug and the rests were on insulin treatment. The blood sugar level (fasting, post- prandial, and HbA1c) were signicantly higher in the case group in comparison to the control group (P<0.001). Conclusion: In our study, the levels of oxidative stress were higher in T2DM individuals compared to controls. These ndings demonstrate a signicant relationship between acute glucose swings and activation of oxidative stress.
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