Ameliorative Effect of Active Principle Isolated from Seeds of Eugenia jambolana on Carbohydrate Metabolism in Experimental Diabetes

Abstract

The aim of this study was to evaluate the antidiabetic activity of LH II purified from ethanolic seed extract of Eugenia jambolana in alloxan-induced mild diabetic (MD) and severely diabetic (SD) rabbits. Ethanolic extract upon chromatographic purification yielded partially purified hypoglycemic principle (SIII) which on further purification by sephadex LH 20 yielded pharmacological active compound LH II. Homogeneity of LH II was tested by HPLC. Phytochemical investigation of LH II by various structural spectra showed the presence of saturated fatty acid, Δ5 lipid and presence of sterol. LH II was administered orally at a dose of 10 mg kg−1 body weight to MD and SD. LH II resulted, significant fall in FBG at 90 min (21.2% MD: 28.6% SD), 7th day (35.6% MD) and 15th day (59.6% SD). Glycosylated hemoglobin was significantly decreased (50.5%) in SD after 15 days treatment (Tt). Plasma insulin levels were significantly increased (P < .001). In vitro studies with pancreatic islets showed 3-fold increase in insulin levels as compared to untreated animals. LH II also showed extrapancreatic effect by significantly increasing (P < .001) the activity of key enzymes of glycolysis and significantly decreasing (P < .001) the activity of key enzymes of gluconeogenesis. Liver and muscle glycogen content were increased by 36.6 and 30% for MD, and 52 and 47% for SD, respectively. Thus, the present study demonstrates that LH II possesses potent antidiabetic activity and it is effective in both MD and SD rabbits.

In vivoassessment of antidiabetic and antioxidative activity of natural phytochemical isolated from fruit-pulp ofEugenia jambolanain streptozotocin-induced diabetic rats

“…STZ-induced diabetic animals also showed suppression in the activity of glycolytic enzymes (hexokinase and phosphofructokinase) with a concomitant elevation in the activity of gluconeogenic enzymes (glucose-6-phosphatase and fructose-1,6-bisphosphatase). These results are consistent with earlier findings in experimental diabetes [11]. Following supplementation with FIIc, the activity of both glycolytic and gluconeogenic enzymes were significantly improved.…”

“…The antihyperglycemic activity of the seeds of E. jambolana is well documented [5][6][7][8][9][10]. Recently, we have demonstrated the antidiabetic effect of active principle isolated from the seeds of E. jambolana [11]. However, studies on the antihyperglycemic activity of fruitpulp of E. jambolana are lacking.…”

Antihyperlipidemic Effect of Active Principle Isolated from Seed ofEugenia jambolanaon Alloxan-Induced Diabetic Rabbits

“…Of the three fractions, only LH II was previously found to possess potent antihyperglycemic activity. 15 Its homogeneity was tested via high-performance liquid chromatography using a GPC column (KF-803, 8Â300 mm i.d. ; Waters, Milford, MA, USA).…”

“…[6][7][8][9][10][11][12][13][14] However, few studies have isolated and studied the active principle from ethanolic seed extract of E. jambolana. In continuation of our research with E. jambolana, 15 we assessed the efficacy of the active principle (fraction II from Sephadex LH 20 chromatography [LH II]) isolated from seeds of E. jambolana on lipidemic control in both mildly diabetic (MD) and severely diabetic (SD) rabbit models of diabetes.…”

Trans-anethole, a terpenoid ameliorates hyperglycemia by regulating key enzymes of carbohydrate metabolism in streptozotocin induced diabetic rats

“…and diagnosis of diabetes [35]. Due to insulin deficiency, the protein content is decreased in muscular tissue by proteolysis [36].…”