Pemeluk agama Islam di Indonesia mencapai 87% dari total jumlah penduduk. Kebutuhan produk halal menjadi hal wajib bagi produsen maupun konsumen muslim, tetapi, kepedulian terhadap kehalalan suatu produk masih sangat rendah. Sertifikasi halal berfungsi tidak hanya sebagai perlindungan konsumen khususnya masyarakat muslim tetapi juga sebagai strategi perdagangan internasional. Pondok Modern Sumber Daya At-Taqwa memiliki Usaha Mikro Kecil dan Menengah (UMKM) yang menghasilkan produk salah satunya adalah Socolat. Namun, produk tersebut belum memiliki sertifikasi halal. Produk Socolat merupakan produk makanan berbahan dasar coklat putih dan daun kelor. Produk ini belum memiliki sertifikat halal. Kegiatan ini bertujuan meningkatkan kemampuan UMKM dari Pondok modern Sumber Daya At-Taqwa (POMOSDA), hingga mampu membawa produk Socolat menuju proses sertifikasi halal. Strategi yang dilakukan antara lain:Melakukan pelatihan kader halal, pemetaan produk, menetapkan titik kritis halal berdasarkan Sistem Jaminan Halal (SJH) dan melakukan pendampingan sampai produk Socolat disubmit seluruh dokumen untuk mendapat sertifikasi halal. Pada akhir kegiatan ini, produk Socolat resmi menjadi binaan Pusat Kajian Halal ITS dan seluruh dokumen ajuan sertifikasi halal beserta kelengkapannya berhasil dibuat dan diajukan kepada Majelis Ulama Indonesia melalui Pusat Kajian Halal ITS.
Cellulase and xylanase were covalently immobilized on chitosan magnetic particles. They were employed as catalysts to produce reducing sugar from coconut husk individually and simultaneously. Fourier Transform Infrared Spectroscopy (FT-IR) and Bradford's protein analysis confirmed that the enzymes were covalently immobilized on the support. Cellulase from Aspergillus niger and Trichoderma reesei immobilized on chitosan magnetic microparticles yielded 0.352 g/L and 0.316 g/L of reducing sugar respectively, while immobilized xylanase from Trichoderma longibachterium yielded 0.432 g/L. The simultaneous use of cellulase and xylanase produced 0.8034 g/L of sugar, and the addition of glutaraldehyde as a cross-linking agent increased the amount of reducing sugar. Enzyme could maintain its activity at 91% for up to five cycles. Using nanosized particles resulted in a sugar yield of 0.49 g/l and 54.7 % of its activity maintained after five cycles.
The effectivity of employing two types of cellulases from Aspergillus niger and Trichoderma resei covalently immobilized on chitosan microparticle was investigated. Reducing sugar from CMC yielded by immobilized cellulase from T. resei alone and A. niger alone was 0.316 g/L and 0.244 g/L, respectively. Simultaneous use of both cellulases shows a significant increase of reducing sugar produced to 1.020 g/L. The effective combination of this two types of cellulases also occurred when coconut husk was used as substrate. A very high enzyme coupling of 92.06% compared to free enzyme was obtained in the immobilization. Addition of GDA not only increased enzyme coupling to 100% but also improved sugar produced. Immobilized cellulase was successfully maintained its activity until 5 cycles
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