Cellulase and Xylanase Immobilized on Chitosan Magnetic Particles for Application in Coconut Husk Hydrolysis

Hamzah, Afan; Ainiyah, Sidratu; Ramadhani, Dwi; Parwita, Gek Ela Kumala; Rahmawati, Yuli; Soeprijanto, Soeprijanto; Ogino, Hiroyasu; Widjaja, Arief

doi:10.14716/ijtech.v10i3.2905

Cited by 12 publications

(6 citation statements)

References 25 publications

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“…First, because it is necessary to coimmobilize the three enzyme families listed above, and second, due to the mechanism of action of cellulases, and the insoluble nature of cellulose, the initial activity of the immobilized biocatalyst is generally low. Several attempts were made for co-immobilization of cellulase components using a broad range of protocols and supports such CLEAs (Bhattacharya and Pletschke, 2014;Dal Magro et al, 2016;Jafari Khorshidi et al, 2016;Li et al, 2012;Periyasamy et al, 2016;Perwez et al, 2019), chitosan (Hamzah et al, 2019;Mandal, 2019;Masoumeh Mohammadi et al, 2019), non-porous materials (as all enzyme is on the surface of the support, they should be able to attack even insoluble substrates) such as magnetic nanoparticles (Ariaeenejad et al, 2020;Asar et al, 2019;Dal Magro et al, 2018;Giannakopoulou et al, 2019;Muley et al, 2020Muley et al, , 2018Raza et al, 2019) and carbon nanotubes (Abraham and Puri, 2020;Li et al, 2019a;Papadopoulou et al, 2019;Raza et al, 2019;Sillu and Agnihotri, 2020), and others. In some cases, some exogenous β-glucosidase is added to the cellulase components mixture to avoid the inhibition caused by cellobiose in some of the cocktails components.…”

Section: Hydrolysis Of Polysaccharidesmentioning

confidence: 99%

Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?

Arana-Peña

Carballares

Morellon-Sterlling

et al. 2021

Biotechnology Advances

182

203

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Section: Hydrolysis Of Polysaccharidesmentioning

confidence: 99%

Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?

Arana-Peña

Carballares

Morellon-Sterlling

et al. 2021

Biotechnology Advances

182

203

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“…As previously studied, FT-IR spectra that indicated enzymes immobilized on CMNP had been investigated. The significant change of peak was found at 3267.54 cm -1 (N-H); 1629.15 cm -1 (C=O); 1027.66 cm -1 (C-N), and 1376.0 cm -1 (C-O) wavelength [14,15]. The illustration of the mechanism of immobilization is shown in figure 2.…”

Section: Enzyme Immobilization On Cmnp and Thermal Stabilitymentioning

confidence: 96%

The effect of surfactant on the hydrolysis of coconut husk using cellulase and xylanase enzyme immobilized on chitosan magnetic nanoparticles

Tiatira

Hamzah

Sangian

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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This study aimed to investigate the effect of surfactant Tween 80 in the enzymatic hydrolysis of coconut husks by using immobilized cellulase from Aspergillus niger, Trichoderma reesei, and immobilized xylanase. Surfactant is common to enhance the yield of sugar in enzymatic process. However, its application in immobilized enzymes has not been well investigated. Coconut husk is an abundant resource of cellulose that can be converted to reducing sugar which can be utilized to produce various products such as biofuel, lactic acid, 5-hydroxymethyl furfural, and leluvinic acid. Chitosan magnetic nanoparticles as a support in the immobilization process were prepared using the coprecipitation method to obtain particles separated by magnetic field. Cellulase and xylanase were immobilized on magnetic particles through covalent bond directly and with Glutaral dialdehyde (GDA) as cross-linker. The immobilized cellulase and xylanase along with Tween 80 surfactants were applied for the hydrolysis of NaOH pre-treated coconut husk at 55°C for 48 hours. The reducing sugar content were identified with DNS methods. In this study, the highest reducing sugar content obtained from cellulase from A niger, cellulase from T reesei and xylanase were 0.412 mg/ml, 0.532 mg/mL and 1.166 mg/mL, respectively. The concentration of Tween 80 that yielding highest sugar concentration for immobilized cellulase from A niger, cellulase from T reesei and xylanase were 2%, 1% and 2% (w/v), respectively.

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“…Previous studies have also confirmed that bioenzymes can be immobilized with magnetic chitosan. The ability to undergo covalent binding produced strong enzyme activity and immobilization was confirmed by characterization of the sample with FTIR (Hamzah et al, 2019).…”

Section: Introductionmentioning

confidence: 93%

Immobilization of Cholesterol Oxidase in Chitosan Magnetite Material for Biosensor Application

Perdani¹,

Juliansyah²,

Putri³

et al. 2020

IJTech

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Cholesterol oxidase, a bio-catalyst that can catabolize cholesterol, has proven applications in medicine. Here, a support material was used to enhance the characteristics of the enzyme. Magnetite (Fe3O4) is widely used as an enzyme support; however, the interaction between the enzyme and the support should be capped with another material, such as chitosan biopolymerbased material. In this study, chitosan-magnetite materials were synthesized by mixing both compounds and activating with glutaraldehyde. The materials were then characterized by Fourier Transform Infrared (FTIR) Spectroscopy. The enzyme kinetic parameters were studied by following the cholesterol oxidation reaction using high-performance liquid chromatography (HPLC) and comparing the results between the free and the immobilized enzyme. The substrate concentration was 2.5 mg/mL. The effect of enzyme concentration was tested using different concentrations of enzyme (0.5, 1, and 2 mg/mL) to determine the best operating conditions. The best conditions for the oxidation reaction were immobilized enzyme at a 2 mg/mL concentration. Enzyme immobilization significantly decreased the optimum substrate concentration to 0.1 mg/mL.

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Cellulase and Xylanase Immobilized on Chitosan Magnetic Particles for Application in Coconut Husk Hydrolysis

Cited by 12 publications

References 25 publications

Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?

Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?

The effect of surfactant on the hydrolysis of coconut husk using cellulase and xylanase enzyme immobilized on chitosan magnetic nanoparticles

Immobilization of Cholesterol Oxidase in Chitosan Magnetite Material for Biosensor Application

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