BackgroundThe present study described the phytochemical profile of Lavandula stoechas essential oils, collected in the area of Ain-Draham (North-West of Tunisia), as well as their protective effects against alloxan-induced diabetes and oxidative stress in rat.MethodsEssential oils samples were obtained from the aerial parts of the plant by hydrodistillation and analyzed by GC–MS. Rats were divided into four groups: Healthy Control (HC); Diabetic Control (DC); Healthy + Essential Oils (H + EO) and Diabetic + Essential Oils (D + EO).Antidiabetic and antioxidant activities were evaluated after subacute intraperitoneally injection of Lavandula stoechas essential oils (50 mg/kg b.w., i.p.) to rats during 15 days.ResultsThe principal compounds detected are: D-Fenchone (29.28%), α-pinene (23.18%), Camphor (15.97%), Camphene (7.83%), Eucapur (3.29%), Limonene, (2.71%) Linalool, (2.01%) Endobornyl Acetate (1.03%). The essential oils also contained smaller percentages of Tricyclene, Cymene, Delta-Cadinene, Selina-3,7(11)-diene. Furthermore, we found that Lavandula stoechas essential oils significantly protected against the increase of blood glucose as well as the decrease of antioxidant enzyme activities induced by aloxan treatment. Subacute essential oils treatment induced a decrease of lipoperoxidation as well as an increase of antioxidant enzyme activities.ConclusionsThese findings suggested that lavandula stoechas essential oils protected against diabetes and oxidative stress induced by alloxan treatment. These effects are in partly due to its potent antioxidant properties.
BackgroundWe aimed in the present study, at investigating the gastroprotective effect of carob pods aqueous extract (CPAE) against ethanol-induced oxidative stress in rats as well as the mechanism implicated.MethodsAdult male wistar rats were used and divided into six groups of ten each: control, EtOH (80 % v/v, 4 g/kg b.w.), EtOH 80 % + various doses of CPAE (500, 1000 and 2000 mg/kg, b.w.) and EtOH + Famotidine (10 mg/kg, p.o.) Animals were perorally (p.o.) pre-treated with CPAE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) for two hours.ResultsThe colorimetric analysis demonstrated that the CPAE exhibited an importance in vitro antioxidant activity against ABTS and DPPH radicals. We found that CPAE pretreatment in vivo, protected against EtOH-induced macroscopic and histological changes induced in stomach mucosa. Carob extract administration also protected against alcohol-induced volume gastric juice decrease. More importantly, We showed that CPAE counteracted EtOH-induced gastric lipoperoxidation, reversed the decrease of sulfhydryl groups (−SH) an hydrogen peroxide (H2O2) levels, and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx).ConclusionsThese findings suggest that CPAE exerted a potential gastro-protective effect against EtOH-induced oxidative stress in rats, due in part, to its antioxidants properties.
International audienceWe aimed in the present study to investigate the protective effect of a myrtle (Myrtus communis L.) berry seed aqueous extract (MBSAE) on acetic acid (AA)-induced colitis in rats as well as the mechanism implicated in this coli-protection. The use of the LC/MS technique allowed us to identify 18 phenolic compounds in the MBSAE. Secondly, we found that the MBSAE inhibited the luminol-amplified chemiluminescence of resting neutrophils and N-formyl-methionylleucyl-phenylalanine (fMLF) or phorbolmyristate acetate (PMA) stimulated neutrophils in a dose-dependent manner. The MBSAE had no effect on superoxide anions, but it inhibited H2O2 production in the cell free system stimulated with horseradish peroxidase (HRPO) and MPO release from the neutrophils. In vivo, the pre-treatment of rats with sulfasalazine (100 mg kg(-1)) and the MBSAE (25, 50, and 100 mg kg(-1)) significantly reduced AA-induced colonic mucosa lesions as well as histopathological changes. The MBSAE counteracted AA-induced lipid peroxidation and the depletion of the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). We also found that the myrtle extract inhibited the increase of the plasma scavenging activity (PSA) and preserved the content of non-enzymatic antioxidants such as sulfhydryl groups (-SH) and reduced glutathione (GSH). More importantly, acetic acid administration increased colonic hydrogen peroxide (H2O2), free iron and calcium levels, while the MBSAE pre-treatment reversed all intracellular mediator perturbations. In conclusion, our data suggests that the MBSAE exerted a potential protective effect against AA-induced injury and oxidative stress in the rat colon. This coli-protection might be related in part to its antioxidant and ROS scavenging activities or by negatively regulating Fenton reaction components such as H2O2 and free iron, which are known to lead to cytotoxicity mediated by intracellular calcium deregulation
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