Gastroprotective effect of carob (Ceratonia siliqua L.) against ethanol-induced oxidative stress in rat

Abstract

BackgroundWe aimed in the present study, at investigating the gastroprotective effect of carob pods aqueous extract (CPAE) against ethanol-induced oxidative stress in rats as well as the mechanism implicated.MethodsAdult male wistar rats were used and divided into six groups of ten each: control, EtOH (80 % v/v, 4 g/kg b.w.), EtOH 80 % + various doses of CPAE (500, 1000 and 2000 mg/kg, b.w.) and EtOH + Famotidine (10 mg/kg, p.o.) Animals were perorally (p.o.) pre-treated with CPAE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) for two hours.ResultsThe colorimetric analysis demonstrated that the CPAE exhibited an importance in vitro antioxidant activity against ABTS and DPPH radicals. We found that CPAE pretreatment in vivo, protected against EtOH-induced macroscopic and histological changes induced in stomach mucosa. Carob extract administration also protected against alcohol-induced volume gastric juice decrease. More importantly, We showed that CPAE counteracted EtOH-induced gastric lipoperoxidation, reversed the decrease of sulfhydryl groups (−SH) an hydrogen peroxide (H2O2) levels, and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx).ConclusionsThese findings suggest that CPAE exerted a potential gastro-protective effect against EtOH-induced oxidative stress in rats, due in part, to its antioxidants properties.

Gastro-protective effect of Ziziphus abyssinica root extracts in ethanol-induced acute ulcer in Wistar rats

“…This shows that the plant is safe and has no toxicity when administered orally up to 5 g/kg. The absence of acute toxicity in rats ensured a good margin of safety for the doses of this extract in the experimental protocols of gastric lesions, as no mortality recorded for each animal [10][11][12].…”

CAROB (Ceratonia siliqua): HEALTH, MEDICINE, CHEMISTRY

“…102 Carob extracts are being studied in recent years for gastroprotective activities, and they were found highly active, so far. [155][156][157][158][159] Most studies were done in vivo after induction of gastric disorder in animals. These diorders were induced by various chemicals such as ethanol, hydrochloric acid, sodium chloride or dextran sulfate sodium.…”

“…155 Aqueous extract of pods was tested for antioxidant activity (DPPH) and against ethanol-induced oxidative gastric stress (lipoperoxidation and hydrogen peroxide increase) in rats and found active. 156 Aqueous extract of pods was tested against dextran sulfate sodiuminduced sub-acute experimental ulcerative colitis in adult Wistar rats. Results were positive and authors attributes this to phenolic compounds in Carob.…”

Farklı Oranlarda Keçiboynuzu Unu İçeren Pestillerin Bazı Fiziksel, Kimyasal ve Duyusal Özelliklerinin Belirlenmesi

“…Toplam kuru maddesi yaklaşık %91-92 oranında olan keçiboynuzu meyvesinde %62-67 oranında şeker, %4-6 protein, %0,2-0,4 ham yağ, %2-3 kül, %4,6-6,2 ham selüloz ve diğer maddeler bulunmaktadır (Karkacıer ve Artık, 1995). Keçiboynuzu meyvesi tüketiminin antioksidan, anti-ishal, antibakteriyel, antiülser ve anti-inflamatuar etkileri de dahil olmak üzere özellikle sindirim sistemi üzerine farmakolojik etkilerinin bulunduğu kanıtlanmıştır (Rtibi et al, 2015a;Rtibi et al, 2016a;Rtibi et al, 2016b;Kivçak ve ark., 2002;Rtibi et al, 2015b;Rtibi et al, 2016c;Rtibi et al, 2017). Besleyici değeri ve sağlık etkileri bu derece yüksek olan keçiboynuzundan kek, pasta, ekmek, makarna, çikolata, peynir, dondurma, meyve jölesi ve marmelat yapımında faydalanılmaktadır (Kirk ve Othmer, 1967;Yurdagel ve Teke, 1985).…”