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Introducción. La anisakidosis es una zoonosis causada por la ingestión accidental de larvas (L3) de anisákidos. Objetivo. Caracterizar el patrón proteico y perfil antigénico de la L3 de Anisakis simplex s.l. (tipo I), A. physeteris s.l. (tipo II) y Contracaecum osculatum s.l. aisladas de peces comerciales. Métodos. Se realizó una corrida electroforética en SDSPAGE de los antígenos somáticos. Se inmunizó conejos experimentalmente y se evaluó por EITB. Resultados. El patrón proteico de Anisakis tipo I mostró 12 bandas, 18 Anisakis tipo II y C. osculatum 13, con las bandas 10 y 35 kDa específicas para Anisakis tipo II, 28 y 77 para C. osculatum no presentes en Anisakis tipo I. Conclusión. se determinó bandas inmunogénicas específicas para Anisakis tipo I con las proteínas de peso molecular 11, 14, 25 y 40 kDa, para el tipo II de 9, 10, 12, 35 y 41 kDa, y C. osculatum 13, 15, 17, 30 y 47 kDa.

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The objective of this study is to use DNA barcoding to relate cystacanths and adults belonging to the acanthocephalans genus Corynosoma found in the southeastern Pacific Ocean off the coast of Peru. For this, we sampled three species of commercial fish (Paralichthys adspersus, Paralabrax humeralis and Cheilodactylus variegatus) and two South American sea lions Otaria byronia stranded on the beaches of the city of Huacho and Barranca, department of Lima. A total of 509 larvae were found in the body cavity of 95 fish (total prevalence 54.28%, total mean intensity 8.64), moreover, a total of 127 adults were found in the large intestine of two South American sea lion (P = 100%, MI = 63.5). We isolated 203 larvae of P. humeralis (P = 65.71%; MI = 8.83; MA = 5.8), 235 (P = 54.29%; MI = 12.37; MA = 6.71) of C. variegatus and 71 (P = 42.86%; MI = 4.73; MA = 2.03) of P. adspersus. All adult and larval specimens were morphologically identified as Corynosoma sp. We performed phylogenetic analysis and generated cytochrome c oxidase subunit 1 (cox1) gene sequences that were compared with sequences available from GenBank. Likewise, using maximum likelihood (ML) and Bayesian inference (BI) revealed that the 16 new sequences (four adults and twelve larvae) were grouped into a clade formed by Corynosoma australe. Therefore, our results contribute to broaden the range of paratenic hosts as well as to record the presence of C. australe in the definitive host. In addition, it is the first analysis that confirms the presence of C. australe using DNA barcoding, allowing its geographical distribution to be extended to the Southeast Pacific Ocean off the coast of Peru.

The Pacific tapeworm Adenocephalus pacifcus (syn. Diphyllobothrium pacificum) is a causative agent of diphyllobothriosis occurred in Pacific coast of South America, mainly in Peru. Source of infections are traditional meal from raw or undercooked marine fish such as “cebiche”. We confirmed 3 new cases, one including scolex and the other two headless. A strobila 46 cm long without scolex was discharged from an 8-year-old boy before treatment. Specimens were confirmed morphologically by presence of tegumental protuberances on proglottids and small sized eggs. Partial sequence of cytochrome c oxidase subunit 1 gene was congruent with A. pacificus sequences.

El 4 de junio de 2021, partió a la eternidad nuestro amigo, colega y maestro, el Dr. Manuel Edmundo Tantaleán Vidaurre, docente universitario, un distinguido parasitólogo, en particular en la helmintología. Este trabajo rinde homenaje al maestro e investigador que con su trayectoria, entre aulas y laboratorios, fue sembrando la curiosidad y el conocimiento científico que inspiró a muchos de sus alumnos y estudiantes.