Molecular identification and epidemiological data of Anisakis spp. (Nematoda: Anisakidae) larvae from Southeastern Pacific Ocean off Peru

Martínez-Rojas, Rosa; Mondragón-Martínez, Aarón; De-Los-Santos, Estrellita Rojas; Neyra, Lidia Luz Cruz; García-Candela, Enrique; Delgado-Escalante, Abraham; Sánchez-Venegas, Jaime

doi:10.1016/j.ijppaw.2021.09.001

Cited by 3 publications

(7 citation statements)

References 41 publications

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“…The other two larval nematode parasites found in the present study are S. physeteris and S. brevispiculata, both have been previously reported to infect sperm whales and fishes [35,48]. Skrjabinisakis physeteris is recorded in the Mediterranean, Pacific, and Atlantic Oceans [11,20,34], and S. brevispiculata is recorded in the South and Central Atlantic Ocean and in the Pacific on the coast of Japan [35]. To date, the only record of a Skrjabinisakis species in the Southeast Pacific was performed by Martínez-Rojas et al [20], who found that S. physeteris parasitizes S. japonicus [20].…”

Section: Discussionsupporting

confidence: 61%

“…The larval stages of this species parasitize various teleost fishes and adult nematodes are found infecting delphinids [47]. In the Southeast Pacific, A. pegreffi is reported, based on morphological and molecular analyses, parasitizing commercial fish species, i.e., Trachurus murphyi, Merluccius gayi, Scomber japonicus, and Seriolela violacea [19,20]. In this study, G. maculutus is a new host record to A. pegreffi in the Southeast Pacific.…”

Section: Discussionmentioning

confidence: 69%

“…The mitochondrial cytochrome c oxidase subunit II gene (mtDNA cox2) was amplified using the primers 210 (5 -CACCAACTCTTAAAATTATC-3 ) and 211 (5 -TTTTCTAGTTATATAGATTGRTTYAT-3 ) [22]. PCR reactions were performed according to Martinez-Rojas et al [20]. PCR products were visualized with Sybergreen (Invitrogen, Eugene, Oregon, EUA) staining before electrophoresis on 1.5% agarose gels.…”

Section: Dna Extraction Pcr Amplification and Dna Sequencingmentioning

confidence: 99%

“…Specific identification of these larvae was based only on morphological data. Recent molecular studies indicate the presence of Anisakis pegreffii, infecting commercial fish from the Peruvian coast [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru

Chero,

Ñacari,

Cruces

et al. 2023

Diversity

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The back cusk eel, Genypterus maculatus (Tschudi, 1846), (Ophiidiformes: Ophiididae) is one of the benthic-demersal fish usually consumed in northern Peru. Here, we identified the third stage (L3) Anisakidae sampled from 29 specimens of G. maculatus captured off the south American Pacific coast, Lambayeque Region, Peru. A total of 20 anisakid nematode larvae were collected on the visceral surface and divided morphologically into three types (Type I–III). These larvae were identified by mtDNA Cox2 sequences analysis, which indicated that corresponded to Anisakis pegreffii Campana-Rouget and Biocca, 1955, Skrjabinisakis physeteris (Baylis, 1923) and S. brevispiculata (Dollfus, 1966) Safonova, Voronova, and Vainutis, 2021, respectively. This is the first record of S. brevispiculata in Peru. The results obtained in this study provide knowledge on the diversity and distribution of Anisakis Dujardin, 1845 and Skrjabinisakis Mozgovoi, 1951, species in the south American Pacific waters and their relevance for public health. In addition, we suggest that combined use of molecular and morphological approaches is needed to characterize L3 anisakid larvae.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 69%

Section: Dna Extraction Pcr Amplification and Dna Sequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru

Chero,

Ñacari,

Cruces

et al. 2023

Diversity

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“…Anisakidosis is a zoonotic disease acquired through eating raw or insufficiently cooked fish infected with third-stage larvae of the genera Anisakis, Pseudoterranova, and Contracaecum (Mattiucci and Nascetti 2008;Bao et al 2017;Shamsi et al 2018). Infections by anisakid larvae (L3) are widespread in Europe and eastern Asia, commonly in Japan, Peru, and Chile, as a result of increasing the consumption of raw or undercooked fish and seafood (Chai et al 2005;Sohn et al 2015;Eiras et al 2018;Martínez-Rojas et al 2021). In humans, these parasites can cause severe gastroenteritis (Shamsi and Butcher 2011;Baptista-Fernandes et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from Red Sea coral trout, Plectropomus areolatus

et al. 2023

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Anisakidosis is a foodborne zoonotic infection induced by members of the family Anisakidae via the consumption of raw or undercooked fish such as sushi and sashimi. Identifying anisakid larval species is critical for the epidemiology and diagnosis of diseases caused by them. This study aimed at identifying Anisakis larvae collected from marine fish in Egyptian waters based on morphological characteristics and molecular analysis. Thirty marine fish bar-cheeked trout, Plectropomus maculatus, were collected from Hurghada, Red Sea, Egypt, to investigate larval nematodes of the genus Anisakis. The larvae were detected encapsulated in the peritoneal cavity and muscle of the fish host. This examination revealed that anisakid larvae naturally infected 19 fish specimens with a prevalence of 63.33% and a mean intensity of 4.1 ± 0.40. Most of them (68 larvae: 71.57%) were found in the musculature. Morphological and morphometric analyses using light and scanning electron microscopy revealed a head region with a prominent boring tooth, inconspicuous lips, and a characteristic protruded cylindrical mucron. All larvae in this study possessed the same morphology as Anisakis Larval type I. Molecular analysis based on ITS region using maximum likelihood and Bayesian phylogenetic methods confirmed them as Anisakis typica. This is the first study to identify A. typica larvae from the commercial fish bar-cheeked trout P. maculatus in Egyptian waters using morphological and molecular methods.

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Molecular identification and prevalence of plerocercoid larvae (Cestoda: Diphyllobothriidae) in some commercial fish species from Peru

Mondragón-Martínez,

Marroquin-Vilchez,

Martínez-Rojas

et al. 2024

Parasitol Res

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Molecular identification and epidemiological data of Anisakis spp. (Nematoda: Anisakidae) larvae from Southeastern Pacific Ocean off Peru

Cited by 3 publications

References 41 publications

Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru

Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru

Morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from Red Sea coral trout, Plectropomus areolatus

Molecular identification and prevalence of plerocercoid larvae (Cestoda: Diphyllobothriidae) in some commercial fish species from Peru

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