Although the number of known HIV-infected students in Madagascar increased significantly between 1989 and 1995, very little is known about student behaviour with regard to AIDS. The study objectives were: to describe Malagasy students' sexual behaviour and condom use; to document students' perceptions about condoms; and to study the relationships between students' socio-demographic characteristics, their perceptions about condoms, and their condom use. The survey used a cross-sectional design and was conducted at the Antananarivo's university campus sites. Anonymous questionnaires were self-administered to 320 randomly selected students.Descriptive statistics and 95% confidence intervals were calculated. Logistic regressions were performed to identify the predictors of condom use. Participants' average age was 24 years. Approximately 80% of the participants reported sexual experiences, and the average age at sexual debut was 19 years. Only 5.7% reported consistent condom use. Common reasons for non-use were steady relationships (75.6%), the perception that condoms were useful only during ovulation periods (8.7%), and the decrease of pleasure (6.4%). The predictors of condom use were male gender, and the perception that condoms were useful during ovulation periods.Risky sexual behaviours with regard to AIDS were prevalent in this community. An HIV prevention programme is recommended. Keywords: Sexual behaviour, condoms, HIV, students, Madagascar. RésuméBien que le nombre connu des étudiants malgaches infectés par le VIH ait largement augmenté entre 1989 et 1995, l'information sur le comportement des étudiants par rapport au SIDA est très limitée. Les objectifs de cette étude furent: 1) décrire le comportement sexuel des étudiants malgaches et l'utilisation du préservatif, 2) documenter les perceptions des étudiants envers les préservatifs, 3) examiner dans quelle mesure l'utilisation du préservatif par les étudiants varie en fonction de leurs caractéristiques sociodémographiques et leurs perceptions envers les préservatifs. Cette étude a été menée dans les sites du campus universitaire d' Antananarivo en utilisant un devis transversal. Un questionnaire anonyme a été auto-administré à 320 étudiants sélectionnés au hasard. Des statistiques descriptives et intervalles de confiance à 95% ont été calculés. La régression logistique a été utilisée pour identifier les déterminants de l'utilisation du préservatif. La moyenne d'âge des participants était de 24 ans. Environ 80% des répondants ont signalé avoir eu des rapports sexuels et l'âge moyen de premiers rapports était de 19 ans. Seulement 5,7% ont rapporté l'utilisation systématique du préservatif. Les raisons de non utilisation furent les suivantes : être dans une relation stable (75,6%), la perception que le préservatif est uniquement utile durant la période d' ovulation (8,7%) et la réduction du plaisir (6,4%). Le genre masculin et l'argument d' ovulation ont été identifiés comme prédicteurs d'utilisation de préservatifs. Les comportements sexuels à risque...

We followed 1000 sex workers in Madagascar for 18 months to assess whether adding female condoms to male condom distribution led to increased protection levels and decreased sexually transmitted infections (STIs). For months 1-6, participants had access to male condoms only; in the final 12 months, they had access to male and female condoms. We interviewed participants about condom use every two months and tested for chlamydia, gonorrhoea and trichomoniasis every six months. Following six months of male condom distribution, participants used protection in 78% of sex acts with clients. Following female condom introduction, protection at months 12 and 18 rose to 83% and 88%, respectively. Aggregate STI prevalence declined from 52% at baseline to 50% at month 6. With the female condom added, STI prevalence dropped to 41% and 40% at months 12 and 18, respectively. We conclude female condom introduction is associated with increased use of protection to levels that reduce STI risk.

This study assessed the phenotypic and allelic profiles of ABO and Rhesus D blood group system among first time blood donors at the National Centre of Blood Supply of Antananarivo. We collected through this retrospective study all data registered during 7 years of practice (from 2003 to 2009). Age and sex were analysed with the result of ABO and RhD screening. They were tested both with Beth Vincent and Simonin tests which were performed in a plate, by using commercial monoclonal antibody (Diaclone(®) et Eryclone(®)), and home-made red cells tests. The Rh D was performed with the same commercial kits. The frequencies of alleles were calculated by using Bernstein method. Data about 45,857 donors were obtained. A male predominance (80.46%) was found and most of our donors were aged <40 (74.92%). 98.90% of the donors were Rh D positive. Phenotypic distribution of each ABO antigen was, respectively, 22.61, 29.66, 6.13 and 41.60% for A, B, AB and O antigen. Allelic frequencies of A, B and O were 0.1559, 0.1987 and 0.6454. These results confirmed the fact that Madagascan population had admixed ethnic origin.

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Purpose:Populations that are frequently in contact with animals such as veterinary students have been demonstrated to be at risk of MRSA carriage.Thus, it is relevant to generate baseline data in MRSA nasal carriage and multidrug resistance among Malagasy veterinary students (Madagascar).Method:A cross-sectional study was carried out among veterinary students coming for laboratory training. After their wise consent, nasal swabs of the anterior nares were carried out; and S. aureuswas isolated by selective chromogenic culture. They were then assessed for antimicrobial susceptibility. Results: Nasal swabs of 155 Malagasy veterinary students (Sex-ratio M/F: 0.91), enabled to isolate 30 (19, 35%) S. aureusstrains, among which 14 (46, 66 %) were méthicillin-resistant (MRSA). Risk factors analysis revealed that history of hospitalization, recent antibiotic intake and frequent contact with animals and livestock workers/veterinarians increase the risk of MRSA nasal carriage. Among MRSA nasal isolates, a high rate of multidrug resistance and particularly an intriguing resistance to gentamycin (20%) and vancomycin (7.14%) were observed. Conclusion: These results suggest that MRSA is spreading in Malagasy community requiringa strategic policy againstmultidrug resistant strains. Key

Reçu le 16 juin 2015 ; accepté le 3 août 2015 © Lavoisier SAS 2015Résumé Les données sur les leucémies aiguës (LA) sont peu nombreuses à Madagascar. Nos objectifs étaient de décrire l'aspect épidémiologique et cytologique des leucémies aiguës au laboratoire d'hématologie du centre hospitalier universitaire Joseph Ravoahangy Andrianavalona d'Antananarivo.Nous avons fait une étude rétrospective descriptive et analytique des dossiers de patients atteints de LA entre Janvier 2013 et Décembre 2014 classés selon les critères du groupe Franco-Américano-Britannique (FAB). Au cours de cette période 357 myélogrammes ont été effectués dont 91 cas (25,5%) de LA. L'âge des patients allait de 2 mois à 79 ans avec une prédominance masculine (ratio=1,38). Les LA touchaient tous les âges avec une fréquence plus élevée chez l'enfant. La cytologie et la cytochimie avaient retrouvé 56% (n=5) de leucémie aiguë myéloïde (LAM), 42.9% (n=39) de leucé-mie aigue lymphoblastique (LAL) et un cas de leucémie aigue biphénotypique. Selon la classification FAB, 36,5% (n=18) des LAM étaient de type M2 et 25% (n=13) étaient de type M1. Il y avait une prédominance du type L2 en ce qui concernait les LAL. La cytologie tient une place importante dans le diagnostic des LA mais l'étude d'autres marqueurs tels la cytogénétique, l'immunophénotypage et la biologie moléculaire est devenue indispensable pour une meilleure prise en charge. Mots clés Leucémies aiguës · Cytologie · Epidémiologie · Classification · BiphénotypiqueAbstract Data on acute leukemia (AL) are few in Madagascar. Our aim was to describe the epidemiologic and cytological appearance of acute leukemia in the hematology laboratory at the University Hospital Joseph Ravoahangy Andrianavalona Antananarivo.We made a descriptive and analytical retrospective study of patient records with LA between January 2013 and December 2014 classified by the criteria of the French-American-British group. During this period, 357 cytological study of bone marrow were performed including 91 cases (25.5%) of AL. The age of patients ranged from 2 months to 79 years with predominance of male patients (ratio= 1,38). The AL affect all ages with a higher frequency in children. Cytology and cytochemistry had recovered 56% (n=5) acute myeloid leukemia (AML), 42,9% (n=39) acute lymphoblastic leukemia (ALL) and one case of biphenotypic acute leukemia. According to criteria's of French-American-British (FAB) group, 36,5% (n=18) of AML were AML 2 and 25% (n=13) were AML 1. There was a predominance of ALL 2 for the ALL. Cytology have an important place in the diagnosis of AL, however, the study of other markers such as cytogenetics, immunophenotyping and molecular biology has become necessary.

Introduction: On contact of antibiotics, S. aureus has gradually acquired multiple antibiotic resistances, including the methicillin (MRSA) and without lose its virulence. The aim of the present study was to report the evolution of resistance of S. aureus to different common antibiotics and to determine the antibiotics active against MRSA. Materials and methods: This is a retrospective and descriptive study for 10 years from January 2005 to December 2014 at the Laboratory of Microbiology of the HU-JRA Antananarivo, the biggest academic hospital located in the capital of Madagascar. All demands for standard bacteriological examination were registered in the laboratory for various bacteriological exams or from samples taken from hospitalized patients and we included all positive cultures for S. aureus. The variables selected and used for the study were community or nosocomial sources of patients and results of susceptibility testing. Results: A total of 906 results from 282 (31.12%) community-acquired and 624 (68.88%) nosocomial infections were studied an average of 100±25strains by year of study. Overall, the prevalence of MRSA was 13.83% (39 of 282 isolates)for community-acquired strains, and 15.70% (98 of 624) for nosocomial infections (p> 0.05)with a total of 29.53%. Resistance rate to trimethoprim-sulfamethoxazole was significantly higher in nosocomial infection than in community-acquired. No significant difference was observed in other antibiotics. Of the 137 MRSA, except vancomycin, fusidic acid is the antibiotic that worked the most in 114 cases (83.21%) followed by gentamicin in 96 cases (70.07%). Apart from ciprofloxacin and tetracycline that we have noticed an increase in resistance rates in 2012 and 2013, almost all antibiotics tested have a stable rate of resistance. Conclusion: The antibiotics tested showed extremely high rates of resistance and that the problem of antibiotic resistance in S. aureus is effective in our center.