This study was carried out to evaluate the efficiency of the Flinders Technology Associates (FTA(®)) card (Whatman(®)) as a sampling device and storage platform for RNA from betanodavirus-infected biological samples (viz., larvae, broodstock, cell culture supernatants and rearing seawater spiked with infected materials). The study showed that FTA cards can be used to detect betanodaviruses by reverse transcription-polymerase chain reaction (RT-PCR). The diagnostic efficiency of RT-PCR from all sample types on FTA cards decreased after 21 days of storage at 4 °C, although the virus could be detected up to 28 days by nested RT-PCR. The FTA card protocol thus provides a supplementary method for quick and easy collection of samples, preservation of RNA on a dry storage basis, and detection of betanodavirus-infected fish.
Shrimp culture being a highly productive aquaculture venture contributes substantially to the export revenue of many Asian countries. At present, the global shrimp industry is dominated by Pacific white shrimp, Penaeus vannamei with a total production of 4.8 million metric tonnes (FAO, 2018). Expansion of P. vannamei culture across the world could be attributed to the availability of fast-growing and specific pathogen-free (SPF) strains mainly from selective breeding programmes. In India, the focus was mainly on the black tiger shrimp, P. monodon till 2009 after which the Pacific white shrimp made its foray into India in a regulated manner, effectively replacing it primarily due to the easy availability of SPF broodstock for shrimp farmers and hatchery operators (Remany
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