Paper-based archiving of biological samples from fish for detecting betanodavirus

Krishnan, A. Navaneeth; Bhuvaneswari, T. V.; Praveena, P. Ezhil; Jithendran, K. P.

doi:10.1007/s00705-016-2875-y

Cited by 10 publications

(9 citation statements)

References 20 publications

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“…Plant samples were assessed in 31.9% of the included articles, and samples obtained from insects corresponded to 14.9%. These works aimed to amplify viral RNA of arboviruses that can cause disease in humans, and also viruses capable of causing disease in fishes and plants (Hall-Mendelin et al, 2010;Price et al, 2014;Flies et al, 2015;Yang et al, 2015;Navaneeth Krishnan et al, 2016;Hall-Mendelin et al, 2017;Melanson et al, 2017;Chikh-Ali et al, 2016). Viral specimens were derived from cell culture or from a commercial inactivated vaccine in 10.6% of the reports (Muthukrishnan et al, 2008;Li et al, 2012;Bankamp et al, 2013;Sakai et al, 2015;Montmayeur et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Cardona-Ospina

Villalba-Miranda

Palechor-Ocampo

et al. 2019

Preventive Veterinary Medicine

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A B S T R A C TBackgroundDetection and characterization of viral RNA pathogens from fieldwork are challenging due to the instability of the RNA molecule. FTA cards® have proved useful for sample storage and latter identification of pathogens with importance for agricultural, animal and human health: however, for optimal handling, processing, and biosafety measures are not well-established.ObjectiveThis systematic review aims to summarize the reported effectiveness of FTA cards® for storage and transport of viral RNA, as well as the conditions for their handling and use in downstream processes. Finally, the biosafety measures required to protect researchers and clinical lab workers are considered.MethodsWe performed a systematic review following the PRISMA statement. We searched MEDLINE (PubMed), Scopus and Web of Science using the keywords "FTA cards" AND "RNA". Articles were screened by title and abstract, and after examination of inclusion and exclusion criteria, relevant information was extracted. The quality of the studies was assessed, and the evidence was qualitatively summarized.ResultsA total of 175 records were retrieved, and 11 additional documents were found by checking references of the eligible articles. A total of 47 articles were included. Samples from animals accounted for 38.3% of the publications, which identified viruses that cause disease in poultry, wild birds, suids, or bovids. Three different methods for RNA extraction were reported. Other factors that vary across reports include the size of RNA amplicon, storage temperature, and duration of storage. Only fourteen articles tested the inactivation of the virus on the FTA card®, and in one case, the virus remained infective.ConclusionFTA cards® could be a suitable option for RNA virus storage and transport for fieldwork in areas where proper conditions for RNA preservation are difficult to achieve. Three different protocols have been used for RNA detection from this matrix. Biospecimens in the form of dried blood spots should be considered potentially infectious unless specifically treated to inactivate viral pathogens. technology consists of filter paper impregnated with a patented chemical mixture that contains chemical denaturants and a free radical scavenger. The chemical mixture lyses cells and organelles, prevents overgrowth of bacteria, and denatures proteins. The DNA is captured in the matrix and remains tightly bound while proteins and inhibitors are washed. This keeps DNA stable during long-term storage at room temperature (GE Healthcare, 2019).

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Section: Resultsmentioning

confidence: 99%

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Cardona-Ospina

Villalba-Miranda

Palechor-Ocampo

et al. 2019

Preventive Veterinary Medicine

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“…The cards can be kept at room temperature, eliminating the need for freezers, excessive storage space and transport of flammable liquids. FTA cards have been successfully used for the preservation of fish buccal cells and mucus, as a cheap alternative to freezing or commercial extraction kits 120,121 . Brown trout ( Salmo trutta ) and northern pike ( Esox Lucius ) DNA was successfully extracted noninvasively with no cross‐contamination from FTA cards 120 .…”

Section: Molecular Techniquesmentioning

confidence: 99%

“…Storage of parasite DNA on FTA cards has been successful, such as with samples containing parasites and parasite eggs 122,123 . DNA can be maintained on cards for years at room temperature and amplified following standard protocols, 124 but experimentally detectable viral RNA (Genus Betanodavirus ) decreased after 4 weeks even when cards were stored at 4°C 121 . A review of 47 studies indicated the maximum storage time for viral RNA on FTA cards ranged from 1 to 8 months at temperatures from −20°C to 37°C 125 .…”

Section: Molecular Techniquesmentioning

confidence: 99%

Moving towards improved surveillance and earlier diagnosis of aquatic pathogens: From traditional methods to emerging technologies

MacAulay

Ellison

Kille

et al. 2022

Reviews in Aquaculture

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Early and accurate diagnosis is key to mitigating the impact of infectious diseases, along with efficient surveillance. This however is particularly challenging in aquatic environments due to hidden biodiversity and physical constraints. Traditional diagnostics, such as visual diagnosis and histopathology, are still widely used, but increasingly technological advances such as portable next generation sequencing (NGS) and artificial intelligence (AI) are being tested for early diagnosis. The most straightforward methodologies, based on visual diagnosis, rely on specialist knowledge and experience but provide a foundation for surveillance. Future computational remote sensing methods, such as AI image diagnosis and drone surveillance, will ultimately reduce labour costs whilst not compromising on sensitivity, but they require capital and infrastructural investment. Molecular techniques have advanced rapidly in the last 30 years, from standard PCR through loop-mediated isothermal amplification (LAMP) to NGS approaches, providing a range of technologies that support the currently popular eDNA diagnosis. There is now vast potential for transformative change driven by developments in human diagnostics. Here we compare current surveillance and diagnostic technologies with those that could be used or developed for use in the aquatic environment, against three gold standard ideals of high sensitivity, specificity, rapid diagnosis, and cost-effectiveness.

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“…Both horizontal and vertical transmission has been demonstrated in various fish species [17]. Persistent or carrier infection may develop to an acute phase with biological and environmental stress factors and facilitate the vertical or horizontal transmission of the nodavirus [18].…”

Section: Nervous Necrosis Virusmentioning

confidence: 99%

Transcriptomic Analysis of Fish Hosts Responses to Nervous Necrosis Virus

2022

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Nervous necrosis virus (NNV) has been responsible for mass mortalities in the aquaculture industry worldwide, with great economic and environmental impact. The present review aims to summarize the current knowledge of gene expression responses to nervous necrosis virus infection in different fish species based on transcriptomic analysis data. Four electronic databases, including PubMed, Web of Science, and SCOPUS were searched, and more than 500 publications on the subject were identified. Following the application of the appropriate testing, a total of 24 articles proved eligible for this review. NNV infection of different host species, in different developmental stages and tissues, presented in the eligible publications, are described in detail, revealing and highlighting genes and pathways that are most affected by the viral infection. Those transcriptome studies of NNV infected fish are oriented in elucidating the roles of genes/biomarkers for functions of special interest, depending on each study’s specific emphasis. This review presents a first attempt to provide an overview of universal host reaction mechanisms to viral infections, which will provide us with new perspectives to overcome NNV infection to build healthier and sustainable aquaculture systems.

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Paper-based archiving of biological samples from fish for detecting betanodavirus

Cited by 10 publications

References 20 publications

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Moving towards improved surveillance and earlier diagnosis of aquatic pathogens: From traditional methods to emerging technologies

Transcriptomic Analysis of Fish Hosts Responses to Nervous Necrosis Virus

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