Emergence of Enterocytozoon hepatopenaei (EHP) in farmed Penaeus (Litopenaeus) vannamei in India

Rajendran, K. V.; Shivam, Saloni; Praveena, P. Ezhil; Rajan, J.J.S.; Kumar, T. Sathish; Avunje, Satheesha; Jagadeesan, V.; Babu, Suresh; Pande, Ashish; Krishnan, A. Navaneeth; Alavandi, S.V.; Vijayan, Koyadan Kizhakedath

doi:10.1016/j.aquaculture.2015.12.034

Cited by 146 publications

(125 citation statements)

References 14 publications

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“…The changes observed by histopathological examinations in the hepatopancreatic tissue were in agreement with the previous reports (Tourtip et al, 2009;Tangprasittipap et al, 2013;Rajendran et al, 2016;Tang et al, 2016). The damage caused to the hepatopancreas affects the metabolic activities and growth of the shrimp, accounting for the retarded growth observed in shrimp ponds with high EHP incidence.…”

Section: Ehp-510r Gcgtactatccccagagcccgasupporting

confidence: 80%

Incidence of Hepatopancreatic Microsporidiasis, by Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei Culture in Nellore District, Andhra Pradesh, India and the Role of Management in its Prevention and Transmission

Kummari¹,

Haridas²,

Handique³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Shrimp culture is the most lucrative aquaculture sector in Asia-Pacific region. Recently, shrimp farms in India have been affected by the microsporidian parasite, Enterocytozoon hepatopenaei (EHP) associated with retarded growth without other clinical signs, causing significant economic losses to the shrimp industry. The present study was carried out to investigate the incidence of EHP in Penaeus vannamei farms of Nellore district of Andhra Pradesh, and to identify the carriers of EHP and the role of management in reducing the incidence of EHP in ponds. PCR analysis by two sets of EHP specific primers indicated the incidence of EHP to be 92.50%. Histopathological studies of the hepatopancreas of affected shrimp showed sloughing of the epithelial cells of hepatopancreatic tubules, lifting and detachment of epithelial layers of hepatopancreatic tubules and heavy damage of hepatopancreas. PCR screening of aquatic macro fauna in the farms showed that samples of crabs, polychaetesand non-penaeids were positive for EHP, implying that they could act as carriers of EHP. On the other hand, fishes were found negative. Strict implementation of Best Management Practices (BMPs) in one selected farm which was confirmed to be infected by EHP clearly showed that practice of BMPs and avoidance of carriers can definitely reduce the incidence of EHP during the subsequent culture in shrimp farms. After implementation of BMPs, significant improvement in growth rate, and Feed Conversion Ratio (FCR) were also observed. In the control pond where BMPs were not implemented, EHP continued to be present, affecting growth and FCR adversely. This is the first report comparing the role of management in EHP infection and identifying carriers of EHP in P. vannamei culture in India. K e y w o r d sEnterocytozoon hepatopenaei,

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Section: Ehp-510r Gcgtactatccccagagcccgasupporting

confidence: 80%

Incidence of Hepatopancreatic Microsporidiasis, by Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei Culture in Nellore District, Andhra Pradesh, India and the Role of Management in its Prevention and Transmission

Kummari¹,

Haridas²,

Handique³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

show abstract

“…Without WFS (39.7%) and With WFS (96.4%) of prevalence was identified in EHP infected ponds (Rajendran et al, 2016). Nevertheless the prevalence of EHP depends on the conditions of culture pond and the prevalence of EHP in Greenhouse ponds (54.4%) was lower than that of in earthen ponds (79.5%) (Shen et al, 2017).…”

Section: Prevalencementioning

confidence: 95%

“…Spore morphology is useful in distinguishing between different species. Spores of EHP appeared oval in shape measuring 1.7-1.0 μm (Rajendran et al, 2016) Approximately 43 microsporidian genera from crustaceans have been described and 11 of these have been reported from shrimps including Agmasoma, Ameson, Enterocytozoon, Inodosporus, Myospora, Perezia, Pleistophora, Thelohania, Tuzetia, Vairimorpha and Vavraia. Microsporidia have been reported from about 20 species of marine or estuarine shrimps and eight species of fresh water crayfish.…”

Section: Microsporidiamentioning

confidence: 99%

“…This parasite is also been reported from China, Indonesia, Malaysia, and Vietnam. In India EHP was first reported by Rajendran et al, (2016). The economic loss in shrimp production due to EHP appears to be significant.…”

Section: Introductionmentioning

confidence: 99%

“…1), White Faeces Syndrome (WFS)and size variation are the major clinical signs of EHP (Rajendran et al, 2016: Salachan et al, 2017. It infects only the tubules of the hepatopancreas in shrimp, and damages the ability of this critical organ to get nutrition from feed.…”

Section: Clinical Signsmentioning

confidence: 99%

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