Hepatopancreatic Microsporidiasis (HPM) in Shrimp Culture: A Review

Kmmari, Suresh; Rathlavath, Srinu; Pillai, Devika; Rajesh, G. K

doi:10.20546/ijcmas.2018.701.383

Cited by 10 publications

(3 citation statements)

References 13 publications

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“…Likewise, there were no clinical signs seen on gross pathology observation may be caused by the infection in the polychaetes specimen we examined was light or not occurred at the same stages as in shrimp. Kummari et al (2018) stated that there were 2 EHP diagnosis approach namely (i) DNA Based and (ii) Microscopic based. DNA based consisted of polymerase chain reaction (PCR) and Loop-Mediated Isothermal Amplification (LAMP), while microscopic based consisted of hematoxylin and eosin, in situ hybridization, scanning electron microscopy, and transmission electron microscopy.…”

Section: Detection Of Ehp In the Polychaete Fresh Smearmentioning

confidence: 99%

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Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks

et al. 2020

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Abstract. Desrina, Prayitno S B, Haditomo A H C, Latritiani R, Sarjito S. 2020. Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks. Biodiversitas 21: 369-374. Enterocytozoon hepatopenaei (EHP) is the newly emerging pathogen of farmed shrimp in Asia. EHP was associated with white feces syndrome (WFS) in shrimp because the spores existed in the feces of shrimp with WFS. Polychaetes are benthic invertebrates and potential to contribute to EHP outbreaks in a pond by acquiring the pathogen and transfer it to shrimp upon feeding. The objective of this study was to determine (1) the occurrence of EHP in polychaetes with Giemsa staining of the coelomic fluid, 1-step PCR and histopathology and (2) to determine pathological changes of EHP infected tissues. Polychaetes were obtained from 20 shrimp ponds located in 9 districts along the North Coast of Java Island, Indonesia. There were no EHP spores detected in the stained coelomic fluid. The 1-step PCR analysis was able to detect the EHP DNA (prevalence was 10%). The Alignment of EHP DNA sequence found in polychaetes showed 100% identity with a published sequence. Histopathological examination showed hypertrophy cells and nuclei, however, no EHP mature spores were found in the epithelial cells on the mucosal layer of the stomach and intestines. Although methods used did not give the convergence results, however, a result of PCR and sequence analysis showed that EHP occurred in the polychaetes in the WFS infected ponds at low point prevalence.

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Section: Detection Of Ehp In the Polychaete Fresh Smearmentioning

confidence: 99%

“…We also observed severe hypertrophy of the epithelial cells that resulted in the epithelial lining separated from the underlying tissues (Figure 2.D). Kummari et al (2018) stated that histologically, there were severe necrotic changes seen in the shrimp hepatopancreas of EHP infected shrimp.…”

Section: Histopathological Examinationmentioning

confidence: 99%

Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks

et al. 2020

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“…The shrimp is one of the most valuable aquaculture species consumed by people from many parts of the world. However, intensive shrimp culture and improper management have led to the outbreak of several diseases, such as acute hepatopancreatic necrosis disease (AHPND), hepatopancreatic microsporidiosis, infectious hypodermal and hematopoietic necrosis, and white spot disease [1][2][3]. AHPND, commonly known as early mortality syndrome, has caused huge loss in the whiteleg shrimp (Litopenaeus vannamei) industry.…”

Section: Introductionmentioning

confidence: 99%

Targeting PirAvp and PirBvp Toxins of Vibrio parahaemolyticus with Oilseed Peptides: An In Silico Approach

Ong

Wong

et al. 2021

Antibiotics

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Acute hepatopancreatic necrosis disease (AHPND), caused by PirAvp- and PirBvp-releasing Vibrio parahaemolyticus strains, has resulted in massive mortality in shrimp aquaculture. Excessive use of antibiotics for AHPND management has led to antibiotic resistance, highlighting the urgency to search for alternatives. Using an in silico approach, we aimed to discover PirAvp/PirBvp-binding peptides from oilseed meals as alternatives to antibiotics. To search for peptides that remain intact in the shrimp digestive tract, and therefore would be available for toxin binding, we focused on peptides released from tryptic hydrolysis of 37 major proteins from seeds of hemp, pumpkin, rape, sesame, and sunflower. This yielded 809 peptides. Further screening led to 24 peptides predicted as being non-toxic to shrimp, fish, and humans, with thermal stability and low water solubility. Molecular docking on the 24 peptides revealed six dual-target peptides capable of binding to key regions responsible for complex formation on both PirAvp and PirBvp. The peptides (ISYVVQGMGISGR, LTFVVHGHALMGK, QSLGVPPQLGNACNLDNLDVLQPTETIK, ISTINSQTLPILSQLR, PQFLVGASSILR, and VQVVNHMGQK) are 1139–2977 Da in mass and 10–28 residues in length. Such peptides are potential candidates for the future development of peptide-based anti-AHPND agents which potentially mitigate V. parahaemolyticus pathogenesis by intercepting PirAvp/PirBvp complex formation.

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Experimental infection of Enterocytozoon hepatopanaei parasite (EHP) of penaeid shrimp in Indian marine crabs

et al. 2022

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Hepatopancreatic Microsporidiasis (HPM) in Shrimp Culture: A Review

Cited by 10 publications

References 13 publications

Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks

Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks

Targeting PirAvp and PirBvp Toxins of Vibrio parahaemolyticus with Oilseed Peptides: An In Silico Approach

Experimental infection of Enterocytozoon hepatopanaei parasite (EHP) of penaeid shrimp in Indian marine crabs

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