Abstract. Desrina, Prayitno S B, Haditomo A H C, Latritiani R, Sarjito S. 2020. Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks. Biodiversitas 21: 369-374. Enterocytozoon hepatopenaei (EHP) is the newly emerging pathogen of farmed shrimp in Asia. EHP was associated with white feces syndrome (WFS) in shrimp because the spores existed in the feces of shrimp with WFS. Polychaetes are benthic invertebrates and potential to contribute to EHP outbreaks in a pond by acquiring the pathogen and transfer it to shrimp upon feeding. The objective of this study was to determine (1) the occurrence of EHP in polychaetes with Giemsa staining of the coelomic fluid, 1-step PCR and histopathology and (2) to determine pathological changes of EHP infected tissues. Polychaetes were obtained from 20 shrimp ponds located in 9 districts along the North Coast of Java Island, Indonesia. There were no EHP spores detected in the stained coelomic fluid. The 1-step PCR analysis was able to detect the EHP DNA (prevalence was 10%). The Alignment of EHP DNA sequence found in polychaetes showed 100% identity with a published sequence. Histopathological examination showed hypertrophy cells and nuclei, however, no EHP mature spores were found in the epithelial cells on the mucosal layer of the stomach and intestines. Although methods used did not give the convergence results, however, a result of PCR and sequence analysis showed that EHP occurred in the polychaetes in the WFS infected ponds at low point prevalence.

Bacterial diseases is frequently occur in catfish culture. The aim of this research was to find out the diversity of causative agent associated with bacterial diseases in catfish based on 16S rDNA gene sequences. The combination between exploratory in the field and experiment, method were applied. Seventeen isolates (D01–D17) were gained from kidney and external wound of moribound catfish with NA and GSP medium that were collected from fish pond of Demak Regency, Indonesia. Based on the postulat results showed that four isolates (D07, D10, D11 and  D14) that  were  caused  10–55% of fishes get sick  and  0–30%  fishes mortal.  On the other hand, there were 13 isolates do not cause both sick and mortality of fish.  On the basis of sequence 16S rDNA analysis, the result showed that D07, D10, D11 and D14 were closely related to Aeromonas caviae (96%), Aeromonas veronii (97%.), Plesiomonas shigelloides (97%) and Pseudomonas putida (96%) respectively. The sensitivity test result indicated that these causative agents have not sensitively to some fish drugs test.

This study aimed at analyzing the relationship between principal supervision and interpersonal communication partially and simultaneously with the work ethic of teachers at Prabumulih Public High School. This research was a survey research with a quantitative approach. The research subject was the teacher while the object of the research was the supervision of the principal and the interpersonal communication. The results obtained indicate that there is a relationship between the principal’s supervision and interpersonal communication partially and simultaneously significant with the work ethic of teachers in SMA Negeri Prabumulih.

Ikan lele adalah salah satu komoditas perikanan air tawar yang menempati urutan teratas dalam jumlah produksi yang dihasilkan. Serangan penyakit pada budidaya ikan lele seperti penyakit Motile Aeromonas Septicemia (MAS) dapat merugikan petani ikan. Penyakit Motile Aeromonas Septicemia (MAS) disebabkan oleh bakteri Aeromonas hydrophila. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman kombinasi ekstrak daun binahong dan bawang putih terhadap gejala klinis dan kelulushidupan ikan lele yang diinfeksi A. hydrophila. Ikan lele yang digunakan sebanyak 180 ekor berukuran 7-9 cm kemudian disuntik bakteri A. hydrophila dengan kepadatan 106 CFU/ml secara intramuskular. Perendaman dilakukan hari ketiga pasca infeksi dengan lama waktu perendaman 10 menit. Dosis dasar yang digunakan yaitu 2500 ppm ekstrak daun binahong dan 1000 ppm ekstrak bawang putih. Rasio dosis perlakuan yang digunakan yaitu perlakuan A (kontrol), perlakuan B (100%:0%), perlakuan C (75%:25%), perlakuan D (50%:50%), perlakuan E (25%:75%), dan perlakuan F (0%:100%). Nilai tingkat kelulushidupan tertinggi hingga terendah setelah dilakukan perendaman yaitu 46,67% (perlakuan D), 20,00% (perlakuan E), perlakuan B, C dan F memiliki tingkat kelulushidupan yang sama yaitu 16,67% dan 10,00% (kontrol).  Perendaman dengan kombinasi ekstrak daun binahong dan bawang putih menunjukkan hasil berpengaruh nyata (P<0,05) terhadap gejala klinis dan kelulushidupan ikan lele. Hasil penelitian menunjukkan bahwa perendaman kombinasi ekstrak daun binahong 1250 ppm dan bawang putih 500 ppm memberikan hasil lebih baik dibandingkan dengan perlakuan lainnya. Kata Kunci: Aeromonas hydrophila, Bawang Putih, Daun Binahong, Ikan Lele Dumbo, Kelulushidupan

Infectious of ectoparasites and vibrios were  found as a problem in fattening mud crab. The aims of this study were to determine the clinical sign of infected mud crabs and to identify the ectoparasites and generic vibrio in the fattening mud crabs from Pemalang Coast, Indonesia. Ectoparasites were collected by smear method. Whereas, 20 isolates were collected from hepatopancreas, gills, hemolymph and injured carapace of the mud crabs. Thiosulphate Citrate Bile Salt Sucrose (TCBS) medium was used to isolate suspected vibrios. The clinical signs of mud crabs infected by ectoparasites were shown through the damaged gills and the attachment of other organisms on the gills, whereas the clinical signs of mud crabs infected by vibriosis were shown through red spot on carapace and wound in carapace, claw and abdomen, followed by weak condition. It also indicated that seven ectoparasites were Ichthyobodo sp., Epistylis sp., Carchesium sp., Vorticela sp., Octolasmis sp., Lepeophtherius sp, and Copepodit sp., whilst five Vibrios were V. harveyi, V. Cholerae, V. parahaemolyticus, V. alginolyticus and V. fischeri.