A polymerase chain reaction (PCR) was developed from sequencing data generated from a specific target band that is unique for Aspergillus fumigatus DNA digested with EcoR1. The target band was detected through Southern blot hybridization of a non-radioactive probe labelled with DIG-dUTP and DNAs of different aspergilli. The DNA of the target band was purified, concentrated and subjected to sequencing. The size of the sequenced band was approximately 445 bp. One pair of primers was designed and synthesized from the sequencing data of the band. The oligonucleotide primers were specific in amplifying an identical band of A. fumigatus in a population mix containing DNAs of different Aspergillus spp.; Pencillium spp.; yeasts; bacterial and viral organisms that are commonly encountered in clinical specimens of respiratory origin. The reaction proved highly sensitive and as little as 0.0001 microgram of A. fumigatus DNA was detected in the reaction.
The current investigation studied an outbreak of lumpy skin disease of cattle in Beni-Suef and Al-Fayium governorates from March up to September 2006. Epidemiological data over a total of 5500 cattle from all ages, breeds and sexes were investigated. Prevalence of lumpy skin antibodies was screened by enzyme-linked immunosorbent assay (ELISA) that revealed high exposure rates; 57% and 51.42% in Beni-Suef and Al Fayium governorates respectively Virus isolation was conducted on chorioallantoic membrane (CAM) of specific pathogen free embryonated chicken egg (SPF-ECE) and MDBK cell culture. The virus identity was confirmed by passive haemagglutination and polymerase chain reaction (PCR) on the infected CAM and MDBK cell culture. Experimental infection of rabbits was successful, demonstrating their possible roles in the epidemiological process of the disease.
In this study field application of RB51 vaccine combined with the policy of test and slaughter as well as application of hygienic measures for control of bovine brucellosis were carried out and evaluated in a dairy herd of cattle for two years. Serological examination of 1280 cattle using tube agglutination, buffered acidified plate antigen, Rose Bengal plate antigen and Rivanol tests revealed 240 (18.75%) positive animals with a previous history of abortion of 12 cows.Brucella melitensis biovar 3 could be isolated from tissue specimens of slaughtered cows. Animals that tested negative in the first examination were vaccinated with RB 51 vaccine with periodical examination every three weeks and slaughtering of positive cases. New positive cows continued to develop up to the 5 th examination then three successive sero-negative tests were obtained with release of the farm from quarantine. Examination of animals 6,12,18 and 24 months post release of quarantine revealed 2, 3, 0 and one positive cases respectively the matter which clarified that the control of the outbreak using RB51 vaccine associated with policy of test and slaughter and application of hygienic measures showed some limitations.
In this study a total of 120 diarrheic buffalo calves were examined clinically and bacteriologically was investigated. The role of E. coli in diarrheic buffalo calves. E. coli, could be isolated from 31 (25.80%) calves. K 99 antigen could be detected in (12.90%) isolates. Studying some virulence factors of E. coli isolates revealed that 28 (90.30) isolates showed congored binding, 29 (93.50%) isolates were able to survive in serum and 23 (74.19%) were able to grow in calf serum, 25 (80.64 %) isolates could be proved as enterotoxin producers and caused accumulation of fluids in the intestinal tract of the inoculated mice. In addition, 28 (90.30 %) were able to produce verotoxins. The present study demonstrated the correlation between the presence of different virulence factors in E. coli isolates and its pathogenicity to newborn calves and its role in diarrheic calves.
A total of 980 camels were employed in this study for evaluation of some diagnostic procedures used for diagnosis of camel trypanosomiasis. Clinical examination revealed that 180 (18.37%) camels showed sings of illness including, loss of body weight, anemia, abortion, decrease of animal production and edema in some parts of the body. Parasitological examination of camel's blood smears revealed the presence of Trypanosoma evansi in 57 (5.82%) camels. ELISA detected 99 (63.06%) positive cases while suratex test identified 80 (50.96%) positive cases. Results of mice inoculation test for detection of Trypanosoma evansi among camels showed that 69 (43.95%) camels were positive. The present study clarified that suratex test was 100% sensitive for diagnosis of trypanosomiasis followed by ELISA (98.55%).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.