A polymerase chain reaction ‘PCR’ for a quick diagnosis of aspergillosis

Gabal, M. A.; El-Sherif, A. M.; Enany, M. S.; Soliman, Sohier S.

doi:10.1046/j.1439-0507.1999.00514.x

Cited by 3 publications

(4 citation statements)

References 20 publications

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“…The presence of Aspergillus fumigatus was determined using previously described PCR primers targeting the A. fumigatus alkaline protease gene. , Positive controls used A. fumigatus (ATCC 34506) genomic DNA as the template, and no-template negative controls were included.…”

Section: Methodsmentioning

confidence: 99%

Centralized Drinking Water Treatment Operations Shape Bacterial and Fungal Community Structure

Vikram

Casson

et al. 2017

Environ. Sci. Technol.

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Drinking water microbial communities impact opportunistic pathogen colonization and corrosion of water distribution systems, and centralized drinking water treatment represents a potential control for microbial community structure in finished drinking water. In this article, we examine bacterial and fungal abundance and diversity, as well as the microbial community taxonomic structure following each unit operation in a conventional surface water treatment plant. Treatment operations drove the microbial composition more strongly than sampling time. Both bacterial and fungal abundance and diversity decreased following sedimentation and filtration; however, only bacterial abundance and diversity was significantly impacted by free chlorine disinfection. Similarly, each treatment step was found to shift bacterial and fungal community beta-diversity, with the exception of disinfection on the fungal community structure. We observed the enrichment of bacterial and fungal taxa commonly found in drinking water distribution systems through the treatment process, for example, Sphingomonas following filtration and Leptospirillium and Penicillium following disinfection. Study results suggest that centralized drinking water treatment processes shape the final drinking water microbial community via selection of community members and that the bacterial community is primarily driven by disinfection while the eukaryotic community is primarily controlled by physical treatment processes.

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Section: Methodsmentioning

confidence: 99%

Centralized Drinking Water Treatment Operations Shape Bacterial and Fungal Community Structure

Vikram

Casson

et al. 2017

Environ. Sci. Technol.

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“…Aspergillus fumigatus is a species that has been intensively investigated in this respect. [11][12][13]22 The species is also commonly found in indoor air. We examined seven pairs of primers designed for the specific detection of A. fumigatus (sets 21-27, Table 1).…”

Section: Species-specific Primersmentioning

confidence: 99%

“…[8][9][10] For medically important fungi, speciesspecific primers are developed to rapidly screen clinical samples and thus promote early diagnosis. [11][12][13][14] Limited numbers of fungal species are usually included in the initial screening during the development of molecular markers currently in use. Clearly, no experiment could include all the fungal species that could be present, because of the enormous diversity of fungi.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of PCR primers and PCR conditions for specific detection of common airborne fungi

Wang

Blomquist

2002

J. Environ. Monitor.

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We examined the selectivity of 53 sets of primers for environmental monitoring of indoor air quality. Thirty-six fungal strains, representing 26 species from 14 genera of commonly occurring fungi, and 16 different bacterial strains, representing both gram-negative and gram-positive species, were included in the experiment. We verified the specificity of 28 of the 53 sets of primers, which were classified as universal fungal, universal bacterial, group or species specific. The PCR conditions required for optimal specificity were also determined. These results can serve as a guide for the step-wise PCR-based detection and identification of airborne fungi commonly found in indoor environments.

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“…The phenotypic features of pathogenic fungi are poor. To resolve this problem, reliable molecular biological methods for species identification 1–4 and strain typing have been developed 5–9 . In this study, we report a case of pulmonary aspergilloma and the molecular biological analysis of isolates from clinical and pathological specimens.…”

Section: Introductionmentioning

confidence: 99%

A case of pulmonary aspergilloma molecular biological identification and typing of the isolates from antemortem sputa and autopsy fungus ball

Sugita

Makimura

Murakami

et al. 2003

Mycoses

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Filamentous fungi were isolated from antemortem sputum and an autopsy fungus ball of the lung in a case of aspergilloma. Both of the isolates were analyzed for the sequences of species or strain-specific nuclear ribosomal DNA (partial 28S and ITS1 regions), and were identified as Aspergillus fumigatus. The molecular biological technique saved time and is thought to be a powerful tool in the accurate diagnosis of pulmonary fungal infection to assure effective treatment.

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A polymerase chain reaction ‘PCR’ for a quick diagnosis of aspergillosis

Cited by 3 publications

References 20 publications

Centralized Drinking Water Treatment Operations Shape Bacterial and Fungal Community Structure

Centralized Drinking Water Treatment Operations Shape Bacterial and Fungal Community Structure

Evaluation of PCR primers and PCR conditions for specific detection of common airborne fungi

A case of pulmonary aspergilloma molecular biological identification and typing of the isolates from antemortem sputa and autopsy fungus ball

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