Epizootiology of lumpy skin disease outbreak in cattle in middle of Egypt, 2006

Abstract

The current investigation studied an outbreak of lumpy skin disease of cattle in Beni-Suef and Al-Fayium governorates from March up to September 2006. Epidemiological data over a total of 5500 cattle from all ages, breeds and sexes were investigated. Prevalence of lumpy skin antibodies was screened by enzyme-linked immunosorbent assay (ELISA) that revealed high exposure rates; 57% and 51.42% in Beni-Suef and Al Fayium governorates respectively Virus isolation was conducted on chorioallantoic membrane (CAM) of specific pathogen free embryonated chicken egg (SPF-ECE) and MDBK cell culture. The virus identity was confirmed by passive haemagglutination and polymerase chain reaction (PCR) on the infected CAM and MDBK cell culture. Experimental infection of rabbits was successful, demonstrating their possible roles in the epidemiological process of the disease.

Molecular characterization, phylogenetic analysis and viral load quantification of Lumpy Skin Disease Virus in Cattle

“…The clinical signs of LSD in suspected cases varied according to severity [32]. Earlier reports revealed classical clinical signs of LSD as fever (102-107℉), salivation, edema of all the limbs, necrotic skin nodule, necrotic wounds in and around the edematous region and sloughing of skin elements in some cases [33,34,35]. We observed peculiar lesions such as brisket edema, edema in limb, dewlap oedema, formation of maggot wound, enlargement in prefemoral lymph node and common clinical signs like fever, salivation, necrotic skin nodule, formation of generalized skin nodules in the whole body and animal become weak, develops downer and chronic disease conditions.…”