Programmed cell death (PCD) is required for many morphological changes, but in plants it has been studied in much less detail than in animals. The unique structure and physiology of the lace plant (Aponogeton madagascariensis) is well suited for the in vivo study of developmental PCD. Live streaming video and quantitative analysis, coupled with transmission electron microscopy, were used to better understand the PCD sequence, with an emphasis on the chloroplasts. Dividing, dumbbell-shaped chloroplasts persisted until the late stages of PCD. However, the average size and number of chloroplasts, and the starch granules associated with them, declined steadily in a manner reminiscent of leaf senescence, but distinct from PCD described in the Zinnia tracheary element system. Remaining chloroplasts often formed a ring around the nucleus. Transvacuolar strands, which appeared to be associated with chloroplast transport, first increased and then decreased. Mitochondrial streaming ceased abruptly during the late stages of PCD, apparently due to tonoplast rupture. This rupture occurred shortly before the rapid degradation of the nucleus and plasma membrane collapse, in a manner also reminiscent of the Zinnia model. The presence of numerous objects in the vacuoles suggests increased macro-autophagy before cell death. These objects were rarely observed in cells not undergoing PCD.

From 2003 to 2006, the blossom level and crop load of ‘Honeycrisp’ apple (Malus × domestica Borkh.) trees on M.26 rootstocks were adjusted to improve fruit quality and return bloom. The treatments consisted of manually removing flower clusters to 50, 100, and 150 per tree, then at ≈50 d after full bloom, the crop load was adjusted to 3, 6, and 9 fruit/cm2 trunk cross-sectional area (TCSA), respectively. All flower and crop load adjustment significantly increased TCSA and canopy volume compared with the control. Classic biennial bearing was observed on the untreated control trees and those thinned to 150 blossom clusters per tree and 9 fruit/cm2 TCSA and was mitigated for trees with 50 and 100 blossom clusters followed by crop load adjustment to 3 and 6 fruit/cm2 TCSA, respectively. Fruit color the “on” year was always lower on the control trees; no difference was found in the “off” year. The treatments increased fruit weight proportional to crop load except for the 2004 “off” year. This study illustrates that for trees with ≈1 m3 canopy volume, the combined effects of blossom and crop load adjustment to 100 blossom clusters/tree followed by fruitlet adjustment to 6 fruit/cm2 TCSA and below will induce consistent annual production for ‘Honeycrisp’.

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