In vivo study of developmental programmed cell death using the lace plant (<i>Aponogeton madagascariensis</i>; Aponogetonaceae) leaf model system

Wright, A. Harrison; Doorn, Wouter G. van; Gunawardena, Arunika H. L. A. N.

doi:10.3732/ajb.0800343

Cited by 40 publications

(39 citation statements)

References 37 publications

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“…Swollen protoplast (black arrow, g), inevitably leading to complete cell lysis (black arrow, h). Scale bars 10 lm (c-f), 20 lm (a, b, g, h) Planta (2011) 233:407-421 413 developmentally regulated system (Wright et al 2009). Wright et al (2009) also made inferences regarding chloroplast fate, but due the thickness of whole lace plant leaves, and the static nature of TEM, were not able to determine if chloroplasts were entering the vacuole to be degraded.…”

Section: Organelles Involved In Pcdmentioning

confidence: 99%

“…Scale bars 10 lm (c-f), 20 lm (a, b, g, h) Planta (2011) 233:407-421 413 developmentally regulated system (Wright et al 2009). Wright et al (2009) also made inferences regarding chloroplast fate, but due the thickness of whole lace plant leaves, and the static nature of TEM, were not able to determine if chloroplasts were entering the vacuole to be degraded. To date, findings of chloroplasts entering the vacuole have been reported in Arabidopsis thaliana (Wada et al 2009), French bean (Minamikawa et al 2001) and wheat (Wittenbach et al 1982), all during developmental PCD leaf senescence.…”

Section: Organelles Involved In Pcdmentioning

confidence: 99%

“…The developmental morphology of the adult lace plant leaf has been divided into five stages as detailed in Gunawardena et al (2004). Due to the predictability of perforation formation, A. madagascariensis makes an ideal model system for the study of developmentally regulated PCD in plants ( Gunawardena et al 2004Gunawardena et al , 2007Gunawardena 2008;Wright et al 2009;Elliott and Gunawardena 2010).…”

Section: Developmentally Regulated Pcd and The Lace Plantmentioning

confidence: 99%

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Environmentally induced programmed cell death in leaf protoplasts of Aponogeton madagascariensis

Lord

Gunawardena

2010

Planta

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Within plant systems, two main forms of programmed cell death (PCD) exist: developmentally regulated and environmentally induced. The lace plant (Aponogeton madagascariensis) naturally undergoes developmentally regulated PCD to form perforations between longitudinal and transverse veins over its leaf surface. Developmental PCD in the lace plant has been well characterized; however, environmental PCD has never before been studied in this plant species. The results presented here portray heat shock (HS) treatment at 55 °C for 20 min as a promising inducer of environmental PCD within lace plant protoplasts originally isolated from non-PCD areas of the plant. HS treatment produces cells displaying many characteristics of developmental PCD, including blebbing of the plasma membrane, increased number of hydrolytic vesicles and transvacuolar strands, nuclear condensation, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling positive nuclei, as well as increased Brownian motion within the vacuole. Results presented here for the first time provide evidence of chloroplasts in the vacuole of living protoplasts undergoing environmentally induced PCD. Findings suggest that the mitochondria play a critical role in the cell death process. Changes in mitochondrial dynamics were visualized in HS-treated cells, including loss of mitochondrial mobility, reduction in ΔΨ(m), as well as the proximal association with chloroplasts. The role of the mitochondrial permeability transition pore (PTP) was examined by pre-treatment with the PTP agonist cyclosporine A. Overall, HS is depicted as a reliable method to induce PCD within lace plant protoplasts, and proves to be a reliable technique to enable comparisons between environmentally induced and developmentally regulated PCD within one species of plant.

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Section: Organelles Involved In Pcdmentioning

confidence: 99%

Section: Organelles Involved In Pcdmentioning

confidence: 99%

Section: Developmentally Regulated Pcd and The Lace Plantmentioning

confidence: 99%

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Environmentally induced programmed cell death in leaf protoplasts of Aponogeton madagascariensis

Lord

Gunawardena

2010

Planta

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“…The structural simplifi cation of vacuoles has been commonly observed in various PCD processes including tracheary element differentiation (Obara et al 2001 ), gibberellin-mediated PCD in central aleurone cells (Guo and Ho 2008 ), embryogenesis in a gymnosperm (Smertenko et al 2003 ), and leaf formation in a lace plant (Gunawardena 2008 ). Decreases in the number of TVS and VM reorganization were confi rmed in the process of developmental PCD in the lace plant Aponogeton madagascariensis (Wright et al 2009 ), which suggests a general role of VM reorganization in vacuolar rupture-mediated PCD in plants.…”

Section: Vacuole-mediated Pcd In Plant Vegetative Developmentmentioning

confidence: 88%

Regulation of Vacuole-Mediated Programmed Cell Death During Innate Immunity and Reproductive Development in Plants

Koyano

Kurusu

Hanamata

et al. 2014

Sexual Reproduction in Animals and Plants

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Programmed cell death (PCD), organized destruction of cells, is essential in development, maintenance of cellular homeostasis, and innate immunity in multicellular organisms. In most angiosperms, development of male and female organs involves spatial and temporal regulation of PCD. The tapetum, the innermost layer of the anther, provides both nutrient and lipid components to developing microspores, and has been proposed to be degraded by PCD during the later stages of pollen maturation. Plants lack homologues of most apoptosis-related genes in animals and have evolved specifi c mechanisms for PCD. PCD is also a crucial event in plant immune responses against microbial infection that prevents the spread of pathogens. Recent live cell imaging techniques have revealed the dynamic features and signifi cant roles of the vacuole during defense responses and PCD. Disintegration or collapse of the vacuolar membrane has been suggested to trigger the fi nal step of

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“…VPEs are thought to mediate the maturation of hydrolytic enzymes within the vacuole, which degrade the tonoplast and initiate a proteolytic executing-PCD cascade (Guicciardi et al 2004;reviewed in Hatsugai et al 2015). Tonoplast rupture is one of the hallmarks of PCD in the lace plant, and it marks the beginning of rapid cell deterioration (Wright et al 2009). …”

Section: Lace Plant Vpe Transcript Levels During Leaf Developmentmentioning

confidence: 99%

Vacuolar processing enzymes, AmVPE1 and AmVPE2, as potential executors of ethylene regulated programmed cell death in the lace plant (Aponogeton madagascariensis)

Rantong

Gunawardena

2018

Botany

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Perforation formation in Aponogeton madagascariensis (Mirb.) H.Bruggen (lace plant) is an excellent model for studying developmentally regulated programmed cell death (PCD). In this study, we isolated and identified two lace plant vacuolar processing enzymes (VPEs) and investigated their involvement in PCD and throughout leaf development. Lace plant VPE transcript levels were determined during seven different stages of leaf development. PCD and non-PCD cells from “window” stage leaves (in which perforations are forming) were separated through laser-capture microscopy and their transcript levels were also determined. VPE activity was also studied between the cell types, through a VPE activity-based probe JOPD1. Additionally, VPE transcript levels were studied in plants treated with an ethylene biosynthesis inhibitor, aminoethoxyvinylglycine (AVG). The two isolated VPEs, AmVPE1 and AmVPE2, are vegetative type VPEs. AmVPE1 had higher transcript levels during a pre-perforation developmental stage, immediately prior to visible signs of PCD. AmVPE2 transcript levels were higher later during window and late window stages. Both VPEs had higher transcript and activity levels in PCD compared with the non-PCD cells. AVG treatment inhibited PCD and associated increases in VPE transcript levels. Our results suggested that VPEs are involved in the execution of the ethylene-related PCD in the lace plant.

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In vivo study of developmental programmed cell death using the lace plant (Aponogeton madagascariensis; Aponogetonaceae) leaf model system

Cited by 40 publications

References 37 publications

Environmentally induced programmed cell death in leaf protoplasts of Aponogeton madagascariensis

Environmentally induced programmed cell death in leaf protoplasts of Aponogeton madagascariensis

Regulation of Vacuole-Mediated Programmed Cell Death During Innate Immunity and Reproductive Development in Plants

Vacuolar processing enzymes, AmVPE1 and AmVPE2, as potential executors of ethylene regulated programmed cell death in the lace plant (Aponogeton madagascariensis)

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