Captive populations of silver carp (Hypophthalmichthys molitrix), a major aquaculture species in Asia, would undoubtedly benefit from genetic monitoring and improvement programs. We report the isolation and preliminary characterization of 16 microsatellite loci derived from both conventional and microsatellite-enriched libraries. Inheritance studies confirmed the allelic nature of observed polymorphisms at all loci, while identifying null alleles at two loci. These loci, having varying degrees of polymorphism, should provide useful markers for applied genetic studies. A high degree of cross-amplification among 10 other cyprinid species suggests that these loci may have more widespread utility
This paper reports a series of trials on the cryopreservation of Oreochromis niloticus (L.) spermatozoa, using methanol as the cryoprotectant.Immotile milt samples pooled from four males were diluted with two diluents, each being subjected to equilibration periods of 15, 30, 45, 60 and 90 min. In addition, samples of fresh pooled milt were kept forO, 2,4,6 and 8 days at 4°C prior to cryopreservation. Diluted samples were stored in 250-jj.l plastic straws and cooled to -50°C at 5°C/min and held under liquid nitrogen for between 1 and 3 weeks. Viability of post-thawed spermatozoa was estimated from video recordings of samples activated under a microscope and from fertilization rates of eggs.The type of diluent and its interaction with equilibration time significantly (P < 0-05) influenced the post-thaw motility of spermatozoa. Spermatozoa stored for up to 6 days prior to cryopreservation were as fertile as freshly frozen spermatozoa and yielded 60-6 (±SE, 8-4) developing embryos.
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