Detection of hybridization between Chinese carp species (Hypophthalmichthys molitrix and Aristichthys nobilis) in hatchery broodstock in Bangladesh, using DNA microsatellite loci

Mia, Md. Younus; Taggart, John B.; Gilmour, A. E.; Gheyas, Almas; Das, Topan K.; Kohinoor, A.H.M.; Rahman, Mohd Muhiden Abd; Sattar, M. A.; Hussain, M. Gulam; Mazid, M. A.; Penman, David J.; McAndrew, Brendan

doi:10.1016/j.aquaculture.2005.02.018

Cited by 55 publications

(44 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, the microsatellite genotype of natural F 1 hybrid showed a weak band from large scale loach allele and a strong band from pond loach allele, a pattern resembling that of the artificial hybrids. The similar phenomenon of amplification asymmetry was also observed in the hybridization between silver carp, Hypophthalmichthys molitrix, and bighead carp, Aristichthys nobilis (Mia et al 2005), although it was noted that they should be recorded with caution to avoid incorrect scoring of unknown stutter bands. More diagnostic microsatellite loci or other molecular markers of the two species are required to identify hybridization events in natural populations in the future.…”

Section: Discussionsupporting

confidence: 55%

Detection of hybridization between two loach species (Paramisgurnus dabryanus and Misgurnus anguillicaudatus) in wild populations

You

Tong

2007

Environ Biol Fish

View full text Add to dashboard Cite

Artificial interspecific hybrids between large scale loach P. dabryanus and tetraploid pond loach M. anguillicaudatus (Cobitidae, Cypriniformes) are viable. To detect the occurrence of possible natural hybridization, genetic analyses by using microsatellite markers were performed for natural populations of large scale loach and pond loach, the reciprocal laboratory hybrids, and "supposed hybrids" with ambiguous morphology. The fertility of the artificial hybrids was also tested. At one diagnostic microsatellite (Mac50), one out of 20 "supposed hybrids" was identified to be F 1 hybrid between the two loach species because it had the same genotype as that of the laboratory hybrids. The triploid hybrids between the two species were confirmed to be female-sterile. The results show that rare hybridization has occurred between diploid large scale loach and tetraploid pond loach in nature although it may have little effect in genetic introgression. This study is helpful for fish conservation and encourages further investigation on natural hybridization and introgression of loaches.

show abstract

Section: Discussionsupporting

confidence: 55%

Detection of hybridization between two loach species (Paramisgurnus dabryanus and Misgurnus anguillicaudatus) in wild populations

You

Tong

2007

Environ Biol Fish

View full text Add to dashboard Cite

show abstract

“…CARMO et al (2015) reported positive results for B. orbignyanus by primers of Salminus brasiliensis and S. franciscanus. However, transferability between genera or families is more difficult due to the genetic distance between specimens (PENTEADO et al, 2011;LOPERA-BARRERO et al, 2016b).…”

Section: Discussionmentioning

confidence: 99%

“…The first solution is time-consuming and costly, limiting the construction of the markers (PENTEADO et al, 2011). Alternatively, primers of related species or phylogenetically close species may be transferred through the annealing of microsatellite sequences (MIA, 2005).…”

Section: Introductionmentioning

confidence: 99%

Cross-amplification of heterologous microsatellite markers in Piracanjuba

et al. 2017

View full text Add to dashboard Cite

Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.

show abstract

“…Microsatellites were widely employed in population genetic studies of numerous species, and this application is continuously expanding (Kohlmann et al, 2005;Mia et al, 2005). Those motifs are often composed of 1-6 bp repeats and there is often a high degree of polymorphism for different numbers of repeats.…”

Section: Introductionmentioning

confidence: 99%

“…Those motifs are often composed of 1-6 bp repeats and there is often a high degree of polymorphism for different numbers of repeats. As microsatellites have some unique characteristics, such as co-dominance of alleles, high allelic diversity, and relatively simple polymerase chain reaction (PCR)-based screening methods that are reproducible, they have been widely used in recent years for population genetic studies of numerous species (Sekino and Hara, 2001;Kohlmann et al, 2005;Mia et al, 2005;Selkoe and Toonen, 2006;Liu et al, 2009). At the same time, microsatellites have been successfully used in genetic mapping and genome analysis (Chen et al, 1997;Li et al, 2000), genotype identification, variety protection seed purity evaluation (Senior et al, 1998), germplasm conservation (Brown et al, 1996), and marker-assisted breeding (Weising et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of microsatellite markers for the lizardfish known as the Bombay duck, Harpadon nehereus (Synodontidae)

Sun²,

Li³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety positive clones from the (CA) 15 -enriched genomic library were sequenced; 62 sequences contained sufficient repeat motifs (di-, tri-and tetra-nucleotide). Twentyeight primer pairs were designed and 21 were successfully amplified; five loci were polymorphic, but with a low number of alleles (three or four). The observed and expected heterozygosities ranged from 0.3500 to 0.8421 and from 0.5244 to 0.6244, respectively. All of the five polymorphic loci were at Hardy-Weinberg equilibrium (adjusted), while linkage disequilibrium between Hane-97 and Hane-175 (P < 0.05, adjusted P value = 0.01) was significant. The low degree of polymorphism of microsatellite markers may be due to the large size of the repeat motifs. These markers will be useful for genetic diversity analysis of H. nehereus.

show abstract

Detection of hybridization between Chinese carp species (Hypophthalmichthys molitrix and Aristichthys nobilis) in hatchery broodstock in Bangladesh, using DNA microsatellite loci

Cited by 55 publications

References 11 publications

Detection of hybridization between two loach species (Paramisgurnus dabryanus and Misgurnus anguillicaudatus) in wild populations

Detection of hybridization between two loach species (Paramisgurnus dabryanus and Misgurnus anguillicaudatus) in wild populations

Cross-amplification of heterologous microsatellite markers in Piracanjuba

Development and characterization of microsatellite markers for the lizardfish known as the Bombay duck, Harpadon nehereus (Synodontidae)

Contact Info

Product

Resources

About