Characterization of microsatellite loci in silver carp (Hypophthalmichthys molitrix), and cross‐amplification in other cyprinid species

Abstract

Captive populations of silver carp (Hypophthalmichthys molitrix), a major aquaculture species in Asia, would undoubtedly benefit from genetic monitoring and improvement programs. We report the isolation and preliminary characterization of 16 microsatellite loci derived from both conventional and microsatellite-enriched libraries. Inheritance studies confirmed the allelic nature of observed polymorphisms at all loci, while identifying null alleles at two loci. These loci, having varying degrees of polymorphism, should provide useful markers for applied genetic studies. A high degree of cross-amplification among 10 other cyprinid species suggests that these loci may have more widespread utility

Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families

“…Sixty‐six microsatellite loci heterozygous in at least one of the eight female breeders were selected to conduct genotyping of progenies of the gynogenetic families (Gheyas, Cairney, Gilmour, Sattra, Das, Mcandrew, Penman & Taggart ; Li, Zhu, Wang, Bai & Yue ; Liao, Yang, Wang, Wang, Zou & Wei ; Liao, Zhang et al . ; Guo, Zou & Yang ; Zhang, Yang, Guo, Wei & Zou ) (Table A2).…”

Construction of a genetic linkage map for silver carp (Hypophthalmichthys molitrix)

“…This included 60 sequences isolated by Lu et al. (2005a,b) for silver carp and bighead carp (30 each, the PCR primers were redesigned in this study); 14 microsatellites developed by Gheyas et al. (2006) and 52 microsatellites developed by Liao et al.…”

Tools for assessing kinship, population structure, phylogeography, and interspecific hybridization in Asian carps invasive to the Mississippi River, USA: isolation and characterization of novel tetranucleotide microsatellite DNA loci in silver carp Hypophthalmichthys molitrix

“…However, no detailed genetic information exists on population structure, levels of effective movement, or relatedness among tributary populations. To address these information needs, we have developed a suite of polymorphic microsatellite DNA loci for the silver carp Hypophthalmichthys molitrix that effectively triples the number of available tetra-nucleotide markers (Tong et al 2002;Das et al 2005;Mia et al 2005;Gheyas et al 2006;Liao et al 2007;Cheng et al 2008). Here we describe the isolation of 25 microsatellite loci, ascertain levels of diversity, heterozygosity, and demographic status of H. molitrix and H. nobolis (bighead), and assess the evolutionary relationships (i.e., cross-species amplification) among four invasive Asian carp species at these loci.…”