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Levadoux (1955) and Branas (1956a,b) and was assumed to be a form of root asphyxia. However, it was this first FD epidemy (Caudwell, 1957) (Milkus, 1974), or by in situ examination such as little leaf (Singh et al, 1975 (Bonfils and Schvester, 1960;Vidano, 1964;Baggiolini et al, 1968; Osler et al, 1975; Caudwell and Larrue, 1979 Gascony (Caudwell, 1957), in Eastern Corsica (Boubals and Caudwell, 1971) and in Pavia, Italy (Belli et al, 1973) in the 1970's and around 1983 in the Languedoc (Heritier, 1983; Caudwell and Larrue, 1986) (Pop, 1968; Rafaila and Costache, 1970; Savin, 1974; Rumbos and Avgelis, 1985).An (Bovey, 1972), and in Germany (Rhine and Mosel Valley) (Gärtel, 1965; Mendgen, 1971

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Flavescence dorée (FD), a grapevine yellows disease, is caused by a mycoplasma-like organism (MLO). A colloidal gold indirect immunolabeling technique identified MLO in salivary glands of a vector leafhopper, Euscelidius variegatus. After aldehyde fixation, tissue samples were prepared by cryoultramicrotomy or embedding in acrylic resins. Double fixation with aldehydes and osmium retroxide, followed by embedding in epon, was also performed. Thin or semi-thin serial sections were treated with polyclonal anti-FD-MLO rabbit antibodies, then with gold-conjugated anti-rabbit IgG. Labeling was revealed using the silver enhancement technique for light microscopy. MLO in frozen thin sections of glands were efficiently labeled. Optimal results were obtained with 4% paraformaldehyde-0.1% glutaraldehyde fixation and low-temperature embedding in LR White resin. Both scattered MLO and unusual dense forms of MLO were easily detected with the electron-dense gold probe. This method distinguished MLO from other membrane-limited bodies and provided a good tool for studying infection in large regions of FD-infected tissues by light microscopy.