The study of starch hydrolysis conditions by immobilized α‐amylase and glucoamylase were carried out. The starch hydrolyzate was applied as a glucose source in the cultivation of Aspergillus niger. Fungus cells were immobilized on the sintered glass support. Such a biocatalizer was used for production of glucose oxidase under the stationary and repeated batch conditions. The results obtained in the cultivation with the immobilized cells were compared with those in the cultivation of the native cells.