Formation and location of glucose oxidase in citric acid producing mycelia of Aspergillus niger

Mischak, Harald; Kubicek, Christian P.; Röhr, M.

doi:10.1007/bf00252357

Cited by 52 publications

(41 citation statements)

References 17 publications

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“…Manganese deficiency affects the anabolism of A. niger, causing a high intracellular ammonium concentration. A decrease in the accumulation of citric acid with iron has been observed, as well as changes in mycelial growth (Mischak et al 1985). Grewal and Kalra (1995) deduced that at a high zinc concentration, the fungi maintained growth without accumulation of citric acid (Drysdale & McKay 1995).…”

Section: Trace Elementsmentioning

confidence: 97%

“…The enzyme is most potent under a high concentration of glucose, with strong aeration and low concentrations of other nutrients (typical conditions for citric acid fermentation) (Mischak et al 1985;Dronawat et al 1995). The effect of glucose oxidase is limited, however, as it is deactivated at pH lower than 3.5.…”

Section: Biochemistry Of Citric Acid Production By Aspergillus Niger mentioning

confidence: 99%

“…Citrate synthase is a catalyst for the reversible condensation reaction between acetyl coenzyme A (acetyl CoA) and oxaloacetate. This reaction favours citrate production and, as a result, thioester hydrolysis occurs during the reaction (Mischak et al 1985). The equation is shown below:…”

Section: Biochemistry Of Citric Acid Production By Aspergillus Niger mentioning

confidence: 99%

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Overview of citric acid production from Aspergillus niger

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Oladele

Siew

et al. 2015

Frontiers in Life Science

215

126

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Citric acid has high economic potential owing to its numerous applications. It is mostly produced by microbial fermentation using Aspergillus niger. In view of surges in demand and growing markets, there is always a need for the discovery and development of better production techniques and solutions to improve production yields and the efficiency of product recovery. To support the enormous scale of production, it is necessary and important for the production process to be environmentally friendly by utilizing readily available and inexpensive agro-industrial waste products, while maintaining high production yields. This article reviews the biochemistry of citric acid formation, choices of citric-acid producing microorganisms and raw materials, fermentation strategies, the effects of various fermentation conditions, citric acid recovery options and the numerous applications of citric acid, based on information drawn from the literature over the past 10 years.

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Section: Trace Elementsmentioning

confidence: 97%

Section: Biochemistry Of Citric Acid Production By Aspergillus Niger mentioning

confidence: 99%

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Overview of citric acid production from Aspergillus niger

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Oladele

Siew

et al. 2015

Frontiers in Life Science

215

126

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“…Mischak (1985) and Petruccioli and Federici (1993); they reported that 8% glucose concentration enhanced the reaction, while Ray and Banik (1999) reported that 15% glucose concentration was affective (Tables 4 and 5). …”

Section: Calculationsmentioning

confidence: 99%

Application of glucose oxidase for the production of metal gluconates by fermentation

Khurshid¹,

Kafiat²,

Hanif³

et al. 2013

Afr. J. Biotechnol.

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The present study deals with the application of glucose oxidase (GOX) for the production of metal gluconates by fermentation method. It provides a method for the conversion of glucose into gluconic acid and its derivatives using the enzyme glucose oxidase (GOX). Due to the presence of calcium carbonate in fermentation medium the gluconic acid is converted into calcium gluconate. Conditions like concentration of substrate, temperature, pH, fermentation period and different phosphate sources were optimized during fermentation. The maximum GOX activity was observed at 35°C (pH 5.5) after 44 h of incubation at 100 rpm. At the maximum enzyme activity, the percentage yield of gluconates are also maximum; both go side by side. Sulphuric and oxalic acids method were employed for the production of gluconic acid. Derivatives of gluconic acid that is, calcium lactate gluconate, sodium gluconate, potassium gluconate, zinc gluconate and copper gluconate were formed by using double displacement and direct methods. The direct method gave the better yield. The percentage yields were 73, 89.63, 81.93, 92.86 and 81.53%, respectively.

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“…The mycelia were grown as described [5]. Escherichia coli strains DH5a [6] and XL1-Blue (Stratagene) were the host strains for the plasraids pUC9 [7] and pBluescript (Stratagene), respectively.…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL‐3

et al. 1989

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A cDNA library from Aspergillus niger strain NRRL‐3 enriched in sequences encoding the glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12 000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5′‐ and a longer 3′‐noncoding region. The deduced protein sequence was verified by partial peptide sequencing.

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Formation and location of glucose oxidase in citric acid producing mycelia of Aspergillus niger

Cited by 52 publications

References 17 publications

Overview of citric acid production from Aspergillus niger

Overview of citric acid production from Aspergillus niger

Application of glucose oxidase for the production of metal gluconates by fermentation

Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL‐3

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