Zur Kenntnis der sogenannten Glucoseoxydase

Franke, Wilhelm; Mchel, Lieselotte; Heye, K.

doi:10.1007/bf00408916

Cited by 4 publications

(4 citation statements)

References 62 publications

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“…The amino terminal sequence had no significant similarities with any other sequences in computer data banks and did not share sequence similarity with the amino acid sequence of yeast allantoinase (Buckholz and Cooper, 1991). Allantoinase activity in soybean seeds was remarkably stable to heat (Table I; Franke et al, 1965), as was found for shoot (Thomas et al, 1983) and nodule allantoinase (Webb, 1988). In this study, 78% of the total activity was retained after seed extracts were heated to 68OC for 7 min (Table I).…”

Section: Characteristics Of Seed Allantoinasesupporting

confidence: 61%

“…In this study, 78% of the total activity was retained after seed extracts were heated to 68OC for 7 min (Table I). Franke et al (1965) found that soybean seed allantoinase was deactivated at 81.5OC. Heat stability has also been reported for allantoinase in seeds of other plants; allantoinase from peanut seeds was active up to 9OoC (Singh et al, 1970) and from Lathyrus sativa it was active up to at least 6OoC (Nirmala and Sastry, 1975).…”

Section: Characteristics Of Seed Allantoinasementioning

confidence: 98%

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Purification of Allantoinase from Soybean Seeds and Production and Characterization of Anti-Allantoinase Antibodies

Webb

Lindell

1993

Plant Physiol.

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Allantoinase catalyzes the hydrolysis of allantoin to allantoic acid, a reaction important in both biogenesis and degradation of ureides. Ureide production in cotyledons of germinating soybean (Glycine max 1.) seeds has not been studied extensively but may be important in mobilizing nitrogen reserves. Allantoinase was purified approximately 2500-fold from a crude extrad of soybean seeds by differential centrifugation, heat treatment, ammonium sulfate fractionation, ethanol fractionation, and fast protein liquid chromatography (Pharmacia) with Mono-Q and Superose columns. The purified enzyme had a subunit size of 30 kD. Polyclonal antibodies produced against the purified protein titrated allantoinase activity in a crude extract of seed proteins. Antibodies recognized the 30-kD band in western blot analysis of crude seed extracts, indicating that they were specific for allantoinase.

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Section: Characteristics Of Seed Allantoinasesupporting

confidence: 61%

Section: Characteristics Of Seed Allantoinasementioning

confidence: 98%

Purification of Allantoinase from Soybean Seeds and Production and Characterization of Anti-Allantoinase Antibodies

Webb

Lindell

1993

Plant Physiol.

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“…In 1965 Franke et al reported first the forma-3 0 tion of (+ )-allantoin in the urate degradation by animal and plant uricases (7). The same compound appears to be formed by the uricase of B.…”

Section: Discussionmentioning

confidence: 98%

Uric acid degradation by Bacillus fastidiosus strains

Bongaerts¹,

Vogels²

1976

J Bacteriol

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Seven Bacillus strains including one of the original Bacillus fastidiosus strains of Den Dooren de Jong could grow on urate, allantoin, and, except one, on allantoate. No growth could be detected on adenine, guanine, hypoxanthine, xanthine, and on degradation products of allantoate. Some strains grew very slowly in complex media. The metabolic pathway from urate to glyoxylate involved uricase, S(+)-allantoinase, allantoate amidohydrolase, S(-)-ureidoglycolase, and, in some strains, urease. In 1929 Den Dooren de Jong (4) described a fastidious Bacillus species that was able to grow only on urate and allantoin. The bacterium was called Bacillus fastidiosus. Similar bacteria were recently isolated by Leadbetter and Holt (9, 12), Claus (according to Kaltwasser [10]), and Mahler (13). The characteristics of some of these strains and four new isolates are compared in this study. Various routes of uric acid degradation by bacteria are known. Except for Veillonella alcalescens (30), Clostridium cylindrosporum, and Clostridium acidiurici (1), all pathways involve allantoin, allantoate, ureidoglycolate, glyoxylate, and urea as intermediates. The enzymes involved and the optical isomers of the various intermediates are different in the various bacteria studied (24). The present paper deals with the uric acid degradation by seven B. fastidiosus strains. MATERIALS AND METHODS Bacterial strains. B. fastidiosus SMG 83 was obtained from H. Kaltwasser (10),

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“…Moreover, a number of natural allantoinases are specific for this optical isomer. Therefore, it is possible that uricase forms S(+)-allantoin (173).…”

Section: Downloaded Frommentioning

confidence: 99%