Zein Increases the Cytoaffinity and Biodegradability of Scaffolds 3D-Printed with Zein and Poly(ε-caprolactone) Composite Ink

Jing, Linzhi; Wang, Xiang; Liu, Hang; Lu, Yuyun; Bian, Jin-Song; Sun, Junying; Huang, Dejian

doi:10.1021/acsami.8b04344

Cited by 66 publications

(58 citation statements)

References 39 publications

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“…Therefore, the functional groups of amino acid residues may enhance the cell affinity with their functional groups. Similarly, Jing et al found that zein incorporation into PCL scaffolds increased degradation of PCL matrix and enhanced the cell affinity on scaffold surface via active amino acid side chains of zein matrix …”

Section: Resultsmentioning

confidence: 94%

Novel zein‐based multilayer wound dressing membranes with controlled release of gentamicin

2018

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Recently, functional multilayer scaffolds with controlled drug release ability come into prominence for wound healing applications to mimic the layered structure of skin tissue and prevent the possible infections at the defect site. In this study, controlled antibiotic releasing zein bilayer membranes were fabricated for treatment of acute skin infections. Gentamicin loaded fibers were prepared by electrospinning on the membrane surface. Membranes were characterized with scanning electron microscope, atomic force microscopy, Fourier transform infrared spectroscopy, contact angle, mechanical analysis, swelling, degradation, and water vapor permeability studies. In vitro cytotoxicity, cell attachment, and proliferation were investigated. Cell attachment on fiber layer was observed with fluorescence imaging. Fabricated fibers showed structural similarity to the skin tissue layers with a fiber diameter range of 350-425 nm and film thickness in the range of 311-361 μm. Mechanical properties were found compatible with the skin tissue. In addition, membranes showed antimicrobial activity against Staphylococcus aureus and Escherichia coli. The sustained release was achieved with a cumulative release of 94%. Membranes did not show any cytotoxic effect. NIH/3T3 and HS2 cell lines were proliferated on each layer mimicking the multilayer skin tissue. Hence, zeinbased bilayer membrane showed promising properties to be used as a potential antimicrobial wound dressing for skin tissue regeneration.

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Section: Resultsmentioning

confidence: 94%

Novel zein‐based multilayer wound dressing membranes with controlled release of gentamicin

2018

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“…Environmental parameters such as temperature and humidity have distinct effects on the cone modes. The cone jet may become dry or continuous discharge when the relative humidity is <50%, since the lower humidity speedup the evaporation rate of PCL/zein composite solutions[ 20 ].…”

Section: Cnn In Ehdp Cone Modes’ Classificationmentioning

confidence: 99%

Electrohydrodynamic printing process monitoring by microscopic image identification

Sun¹,

Jing²,

Fan³

et al. 2018

IJB

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Electrohydrodynamic printing (EHDP) is able to precisely manipulate the position, size, and morphology of micro-/nano-fibers and fabricate high-resolution scaffolds using viscous biopolymer solutions. However, less attention has been paid to the influence of EHDP jet characteristics and key process parameters on deposited fiber patterns. To ensure the printing quality, it is very necessary to establish the relationship between the cone shapes and the stability of scaffold fabrication process. In this work, we used a digital microscopic imaging technique to monitor EHDP cones during printing, with subsequent image processing algorithms to extract related features, and a recognition algorithm to determine the suitability of Taylor cones for EHDP scaffold fabrication. Based on the experimental data, it has been concluded that the images of EHDP cone modes and the extracted features (centroid, jet diameter) are affected by their process parameters such as nozzle-substrate distance, the applied voltage, and stage moving speed. A convolutional neural network is then developed to classify these EHDP cone modes with the consideration of training time consumption and testing accuracy. A control algorithm will be developed to regulate the process parameters at the next stage for effective scaffold fabrication.

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“…Moreover, As a plant-derived protein, zein offers superior bio-stability and less possibility to transmit zoonotic disease compared with animal proteins, such as silk fibroin and collagen 23. Furthermore, zein enhances cell viability, attachment, and proliferation,28 because of the functional groups in amino acid side chains, which could act as cells recognition sites for attachment 29. However, its ability to stimulate cell proliferation needs to be improved 4,30.…”

Section: Discussionmentioning

confidence: 99%

<p>Electrospun Poly (Aspartic Acid)-Modified Zein Nanofibers for Promoting Bone Regeneration</p>

Liu¹,

Miao²,

Qin³

et al. 2019

IJN

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BackgroundCritical-sized bone defects raise great challenges. Zein is of interest for bone regeneration, but it has limited ability to stimulate cell proliferation. In this regard, a poly (aspartic acid) (PAsp)-zein hybrid is promising, as PAsp can promote rat bone marrow stromal cell (rBMSCs) proliferation and osteogenic differentiation. This research aimed to develop electrospun PAsp-modified zein nanofibers to realize critical-sized bone defects repair.MethodsThree groups of PAsp-modified zein nanofibers were prepared, they were PAsp grafting percentages of 0% (zein), 5.32% (ZPAA-1), and 7.63% (ZPAA-2). Using rBMSCs as in vitro cell model and SD rats as in vivo animal model, fluorescence staining, SEM, CCK-8, ALP, ARS staining, μCT and histological analysis were performed to verify the biological and osteogenic activities for PAsp-modified zein nanofibers.ResultsAs the Asp content increased from 0% to 7.63%, the water contact angle decreased from 129.8 ± 2.3° to 105.5 ± 2.5°. SEM, fluorescence staining and CCK-8 assay showed that ZPAA-2 nanofibers had a superior effect on rBMSCs spreading and proliferation than did zein and ZPAA-1 nanofibers, ALP activity and ARS staining showed that ZPAA-2 can improve rBMSCs osteogenic differentiation. In vivo osteogenic activities was evaluated by μCT analysis, HE, Masson and immunohistochemical staining, indicating accelerated bone formation in ZPAA-2 SD rats after 4 and 8 weeks treatment, with a rank order of ZPAA-2 > ZPAA-1 > zein group. Moreover, the semiquantitative results of the Masson staining revealed that the maturity of the new bone was higher in the ZPAA-2 group than in the other groups.ConclusionElectrospun PAsp-modified zein can provide a suitable microenvironment for osteogenic differentiation of rBMSCs, as well as for bone regeneration; the optimal membrane appears to have a PAsp grafting percentage of 7.63%.

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Zein Increases the Cytoaffinity and Biodegradability of Scaffolds 3D-Printed with Zein and Poly(ε-caprolactone) Composite Ink

Cited by 66 publications

References 39 publications

Novel zein‐based multilayer wound dressing membranes with controlled release of gentamicin

Novel zein‐based multilayer wound dressing membranes with controlled release of gentamicin

Electrohydrodynamic printing process monitoring by microscopic image identification

<p>Electrospun Poly (Aspartic Acid)-Modified Zein Nanofibers for Promoting Bone Regeneration</p>

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