Novel zein‐based multilayer wound dressing membranes with controlled release of gentamicin

Abstract

Recently, functional multilayer scaffolds with controlled drug release ability come into prominence for wound healing applications to mimic the layered structure of skin tissue and prevent the possible infections at the defect site. In this study, controlled antibiotic releasing zein bilayer membranes were fabricated for treatment of acute skin infections. Gentamicin loaded fibers were prepared by electrospinning on the membrane surface. Membranes were characterized with scanning electron microscope, atomic force microscopy, Fourier transform infrared spectroscopy, contact angle, mechanical analysis, swelling, degradation, and water vapor permeability studies. In vitro cytotoxicity, cell attachment, and proliferation were investigated. Cell attachment on fiber layer was observed with fluorescence imaging. Fabricated fibers showed structural similarity to the skin tissue layers with a fiber diameter range of 350-425 nm and film thickness in the range of 311-361 μm. Mechanical properties were found compatible with the skin tissue. In addition, membranes showed antimicrobial activity against Staphylococcus aureus and Escherichia coli. The sustained release was achieved with a cumulative release of 94%. Membranes did not show any cytotoxic effect. NIH/3T3 and HS2 cell lines were proliferated on each layer mimicking the multilayer skin tissue. Hence, zeinbased bilayer membrane showed promising properties to be used as a potential antimicrobial wound dressing for skin tissue regeneration.

Air-Jet Spun Corn Zein Nanofibers and Thin Films with Topical Drug for Medical Applications

“…Corn zein is a unique material for nanofibers due to its high tensile strength, flexibility, and toughness in films, [32][33][34][35][36], nanofibers [24], and composites [37]. Fabrication of the fibers is also made easy by zein's hydrophobicity and insolubility in water.…”

Advancements and future directions in the antibacterial wound dressings – A review

“…The healing performance of the antibacterial wound dressing that delivers antibiotics depends on the drug release profile, the physicochemical properties of the drug and the polymeric material. For the effective treatment of the wound infections, the initial burst release, which means the release of about 60% of the loaded drug molecules during the first 5 hr is required followed by the controlled release 69,70 . In the literature, the antibiotics that were generally utilized for antibacterial wound dressing were summarized as gentamicin, 71,72 ciprofloxacin, 25,73 tetracycline, 74,75 and ampicillin 76 …”

“…For the effective treatment of the wound infections, the initial burst release, which means the release of about 60% of the loaded drug molecules during the first 5 hr is required followed by the controlled release. 69,70 In the literature, the antibiotics that were generally utilized for antibacterial wound dressing were summarized as gentamicin, 71,72 ciprofloxacin, 25,73 tetracycline, 74,75 and ampicillin. 76 In the Figure 3a, the schematic preparation route of a novel wound dressing using silk incorporated with gold nanoparticles with subsequent loading of gentamicin sulfate was demonstrated.…”

Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications

“…Because of the merits of plant proteins, increasing attempts are being made to probe possibilities to develop such biomaterials (including micro and nanofibers, hydrogels, porous, and micro and nanoparticles) from numerous plant derived proteins for tissue engineering applications [13,14], drug delivery systems, [15,16] and wound dressings [17,18,19]. However, plant proteins (particularly soy proteins and gluten) possess confined organic solvents (albeit much less toxic than traditional polymeric tissue engineering solvents such as chloroform), and thus, it is hard to produce biomaterials from such proteins.…”