Yogurt Containing the ProbacteriaLactobacillus acidophilusCombined with Natural Antioxidants Mitigates Doxorubicin-Induced Cardiomyopathy in Rats

Abstract

Probiotics and antioxidants have a definite improving effect in cardiovascular diseases. This study aims at mitigating doxorubicin toxicity on cardiac function through consuming a functional food. Five groups of adult male Sprague-Dawley rats were used along 22 weeks. Group I received 30 g/kg/day food enriched with yogurt, green tea extract, and carrots (80, 0.84, and 100 g/kg diet, respectively) from the first week, group II received carvedilol 30 mg/kg/day orally from week 17, group III received both carvedilol and tested food, and groups IV and V were +ve and -ve control groups, respectively. In week 17, cardiomyopathy was induced by i.p. injection of 2.5 mg/kg doxorubicin every 48 h for 2 weeks. Histopathological and electrophysiological examinations and biochemical analysis were done. Lipid peroxidation, antioxidant effect, heart failure compensatory mediators, and proinflammatory cytokines were assessed. Tested food normalized time between the start of Q wave and the end of T wave on electrocardiogram (QT interval) and heart rate compared to the doxorubicin group (P<.05). It also improved hypertrophy indicated by a significant (P<.05) decrease in heart/body weight ratio, angiotensin-II (Ang-II), and atrial natriuretic peptide (ANP) serum levels. Histopathological examination of cardiac sections from the tested food group revealed less marked vacuolization and low perivascular fibrosis percentage (0.7803 ± 0.04). A significant (P<.001) decrease in serum creatine kinase-membrane bound, lactate dehydrogenase, triglycerides, cholesterol, low-density lipoprotein cholesterol, and tissue malondialdehyde (MDA) levels was observed in addition to an increase in serum Na(+)/K(+) ATP1A1 and cardiac reduced glutathione (GSH) levels. Tested food also lowered the inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) serum levels significantly (P<.01). Probiotic food containing Lactobacillus acidophilus, green tea, and carrots can improve membrane integrity and cardiac contractility in doxorubicin-induced cardiomyopathy by decreasing TNF-α, IL-6, MDA, increasing GSH, and modulating compensatory mediators such as Ang-II and ANP.

Effects of Lactobacillus acidophilus on Pituitary-thyroid Axis in Growing Rat

“…Reducing lipid peroxidation and protecting the liver from oxidative effects of free radicals will be one of the main strategies to promote conversion of T4 to T3 by using herbs (Sage, 2013). Meanwhile L. acidophilus proved its effectiveness as antioxidant (Abu-Elsaad et al, 2015) this has resulted in appositive way to increase the conversion of T4 to T3 in the present experiment. Same results recorded by (Chenhui et al, 2014) who found that feeding Se-probiotics caused increase in T3 and not T4.…”

Curcumin Downregulates Phosphate Carrier and Protects against Doxorubicin Induced Cardiomyocyte Apoptosis

“…H9c2 cells were incubated with the same concentration of doxorubicin and subsequently cotreated with various concentrations of curcumin for 2 h. At the end of the treatment, the cells were harvested and sonicated with phosphate buffer (PBS, pH 6.8) containing 1 mmol phenylmethanesulfonyl fluoride (PMSF) to obtain cell homogenates. The thiobarbituric acid-reactive substances (TBARS) method was used to estimate the cellular malondialdehyde (MDA) levels by measuring the absorbance at 532 nm with a spectrophotometer [ 16 ].…”

Yogurt and green tea regimen in the preventions of heart disease and cancer in men

“…Probiotic food comprising yogurt, green tea extract and carrots improved membrane integrity and cardiac contractility in doxorubicin-induced cardiomyopathy. 1 Tea drinking was significantly associated with lower overall cancer and cardiovascular mortality in men, but demonstrated no association with mortality in women. 2 The combination of yogurt and green tea in the preventions of heart disease and cancer has not been well documented.…”