| Several studies have shown that gut microbiota can activate neural pathways and central nervous system signalling systems via gut-brain axis, accordingly the present study was aimed to investigate the interaction of Lactobacillis acidophillus on the activity of pituitary-thyroid axis in the case addressed after weaning of male rats. The study was carried out on growing male rats. At the day of weaning 12 male kids at age of 30 days were divided into two equal groups and treated as following: 1 st group considered as control receiving 1ml of water, 2 nd -group received 1ml of MRS (5x10 8 CFU) of L. acidophilus. All the experimental animals were administered orally and daily for the next 32 days. Body weights were recorded at the beginning and end of the experiment. Blood and thyroid gland samples were collected for hormonal assay at the end of the experiment. There was significant differences in body weight gain. Treated group showed less body weight gain than control group. TSH and T3 increased significantly while GH, T4, and TFI were increased slightly in treated group. The analysis of the photomicrograph of thyroid tissue in lactobacillus received group showed increase in the number and size of the thyroid follicles, in addition to a visible hyperchromatic of the follicles epithelia. In conclusion, feeding of Lactobacillus acidophilus to rat kids showed tangible changes in the pituitary-thyroid axis activity suggesting a positive interaction between Lactobacillus acidophilus and host gut-brain axis.
Background: Consumption of food contaminated with Aflatoxin-B1(AF-B1), caused deleterious effects on different body’s systems ,specially GIT in direct and indirect manners. To investigate the protective role of propolis against AF-B1 effects on rats gut, 24 male rats divided into equal 4 groups: group (C )was control, second group (AF) received Aflatoxin-B1 ( 0.025mg/kg ), third group( P) received Propolis in dose (50 mg /kg BW) and the fourth group( AFP) received Aflatoxin-B1 (0.025 mg/ 1kg BW) + Propolis (50 mg / 1kg BW).after 60 days of experiment , intestinal D-zylose adsorption and histomorphological changes were measured Results: Results revealed that intestinal absorption function of D-Xylose sugar was reduced in AFB-1 exposed rats , meanwhile Propolis improve D-xylose absorption in rats exposed to the AFB-1. Histomorphological measurements include mucosa thickness, Villus high, Crypts depth, Villus/crypt ratio, and goblet cells density in the three portions of small intestine decreased significantly in AF group and increased significantly in P group and kept semi-normal in AFP group in comparison with the control group. Analysis of light microscopic photograph revealed that administration of aflatoxin-B1 cause deleterious changes in stomach, and intestinal tissues, while Propolis was efficient in improving these changes to normal condition. Conclusions: Intestinal absorptive function and histomorphological measurements were altered by Aflatoxin-B1, and the duodenum was the most affected part from the intestine. Results of propolis showed a high activity to improve all the above measurements and counter the deleterious effect of Aflatoxin-B1 to normal situation.
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